Effect of Sodium Benzoate on Apoptosis and Mitochondrial Membrane Potential After Aluminum Toxicity in PC-12 Cell Line
Background: Sodium benzoate, a food preservative, prevents the growth of fungi and bacteria. Numerous studies have reported the protective effects of sodium benzoate on the nervous system. This study investigated the effect of sodium benzoate on cell apoptosis and mitochondrial function in an alumin...
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2020-10-01
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| Series: | Iranian Journal of Toxicology |
| Subjects: | |
| Online Access: | http://ijt.arakmu.ac.ir/article-1-839-en.html |
| _version_ | 1818508919512760320 |
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| author | Marzieh Rashedinia Jamileh Saberzadeh Forouzan Khodaei Najmeh Mashayekhi Sardoei Mahshid Alimohammadi Rita Arabsolghar |
| author_facet | Marzieh Rashedinia Jamileh Saberzadeh Forouzan Khodaei Najmeh Mashayekhi Sardoei Mahshid Alimohammadi Rita Arabsolghar |
| author_sort | Marzieh Rashedinia |
| collection | DOAJ |
| description | Background: Sodium benzoate, a food preservative, prevents the growth of fungi and bacteria. Numerous studies have reported the protective effects of sodium benzoate on the nervous system. This study investigated the effect of sodium benzoate on cell apoptosis and mitochondrial function in an aluminum cell toxicity model.
Methods: After 48 hr of treating PC-12 cells with varying concentrations of sodium benzoate (0.125, 0.25 or 0.5 mg/ml) in the presence of aluminum maltolate (500 μM), the cell viability was assessed by MTT assay. The type of cell death (necrosis or apoptosis) was analyzed by flow cytometry (7-ADD/An V-PE staining). Also, rhodamine 123 was used to measure the Mitochondrial Membrane Potential (MMP). The acetylcholinesterase activity (AChE) was assessed by Ellman’s method.
Results: It was observed that sodium benzoate inhibited aluminum induced cell death within 48hr. Sodium benzoate at a concentration of 0.5 mg/ml significantly reduced the apoptotic cells that had been exposed to aluminum. Exposure of PC-12 cells with sodium benzoate and aluminum, increased the AChE activity, although, no significant changes in mitochondrial membrane potential were observed.
Conclusion: Sodium benzoate may provide its protective effects through increased AChE activity and inhibiting apoptosis induced by aluminum toxicity. It is likely that the disruption of MMP is neither involved in the induction of apoptosis by aluminum nor in the protective effect of sodium benzoate. |
| first_indexed | 2024-12-10T22:38:30Z |
| format | Article |
| id | doaj.art-3e302bfc3dae4d3082fb07e8358abd3a |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-12-10T22:38:30Z |
| publishDate | 2020-10-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-3e302bfc3dae4d3082fb07e8358abd3a2022-12-22T01:30:47ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592020-10-01144237244Effect of Sodium Benzoate on Apoptosis and Mitochondrial Membrane Potential After Aluminum Toxicity in PC-12 Cell LineMarzieh Rashedinia0Jamileh Saberzadeh1Forouzan Khodaei2Najmeh Mashayekhi Sardoei3Mahshid Alimohammadi4Rita Arabsolghar5 Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. Background: Sodium benzoate, a food preservative, prevents the growth of fungi and bacteria. Numerous studies have reported the protective effects of sodium benzoate on the nervous system. This study investigated the effect of sodium benzoate on cell apoptosis and mitochondrial function in an aluminum cell toxicity model. Methods: After 48 hr of treating PC-12 cells with varying concentrations of sodium benzoate (0.125, 0.25 or 0.5 mg/ml) in the presence of aluminum maltolate (500 μM), the cell viability was assessed by MTT assay. The type of cell death (necrosis or apoptosis) was analyzed by flow cytometry (7-ADD/An V-PE staining). Also, rhodamine 123 was used to measure the Mitochondrial Membrane Potential (MMP). The acetylcholinesterase activity (AChE) was assessed by Ellman’s method. Results: It was observed that sodium benzoate inhibited aluminum induced cell death within 48hr. Sodium benzoate at a concentration of 0.5 mg/ml significantly reduced the apoptotic cells that had been exposed to aluminum. Exposure of PC-12 cells with sodium benzoate and aluminum, increased the AChE activity, although, no significant changes in mitochondrial membrane potential were observed. Conclusion: Sodium benzoate may provide its protective effects through increased AChE activity and inhibiting apoptosis induced by aluminum toxicity. It is likely that the disruption of MMP is neither involved in the induction of apoptosis by aluminum nor in the protective effect of sodium benzoate.http://ijt.arakmu.ac.ir/article-1-839-en.htmlsodium benzoatealuminumneurotoxicitypc-12 cell linenecrosis and apoptosisacetylcholinesterase |
| spellingShingle | Marzieh Rashedinia Jamileh Saberzadeh Forouzan Khodaei Najmeh Mashayekhi Sardoei Mahshid Alimohammadi Rita Arabsolghar Effect of Sodium Benzoate on Apoptosis and Mitochondrial Membrane Potential After Aluminum Toxicity in PC-12 Cell Line Iranian Journal of Toxicology sodium benzoate aluminum neurotoxicity pc-12 cell line necrosis and apoptosis acetylcholinesterase |
| title | Effect of Sodium Benzoate on Apoptosis and Mitochondrial Membrane Potential After Aluminum Toxicity in PC-12 Cell Line |
| title_full | Effect of Sodium Benzoate on Apoptosis and Mitochondrial Membrane Potential After Aluminum Toxicity in PC-12 Cell Line |
| title_fullStr | Effect of Sodium Benzoate on Apoptosis and Mitochondrial Membrane Potential After Aluminum Toxicity in PC-12 Cell Line |
| title_full_unstemmed | Effect of Sodium Benzoate on Apoptosis and Mitochondrial Membrane Potential After Aluminum Toxicity in PC-12 Cell Line |
| title_short | Effect of Sodium Benzoate on Apoptosis and Mitochondrial Membrane Potential After Aluminum Toxicity in PC-12 Cell Line |
| title_sort | effect of sodium benzoate on apoptosis and mitochondrial membrane potential after aluminum toxicity in pc 12 cell line |
| topic | sodium benzoate aluminum neurotoxicity pc-12 cell line necrosis and apoptosis acetylcholinesterase |
| url | http://ijt.arakmu.ac.ir/article-1-839-en.html |
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