Identification of Genes Involved in Antifungal Activity of <i>Burkholderia seminalis</i> Against <i>Rhizoctonia solani</i> Using Tn5 Transposon Mutation Method
<i>Rhizoctonia solani</i> is the causative agent of rice sheath blight disease. In a previous study, we found that the growth of <i>R. solani</i> was inhibited by <i>Burkholderia seminalis</i> strain R456. Therefore, the present study was conducted to identify the...
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MDPI AG
2020-09-01
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| author | Muchen Zhang Xiaoxuan Wang Temoor Ahmed Mengju Liu Zhifeng Wu Jinyan Luo Ye Tian Hubiao Jiang Yanli Wang Guochang Sun Bin Li |
| author_facet | Muchen Zhang Xiaoxuan Wang Temoor Ahmed Mengju Liu Zhifeng Wu Jinyan Luo Ye Tian Hubiao Jiang Yanli Wang Guochang Sun Bin Li |
| author_sort | Muchen Zhang |
| collection | DOAJ |
| description | <i>Rhizoctonia solani</i> is the causative agent of rice sheath blight disease. In a previous study, we found that the growth of <i>R. solani</i> was inhibited by <i>Burkholderia seminalis</i> strain R456. Therefore, the present study was conducted to identify the genes involved in the antifungal activity of <i>B. seminalis</i> strain R456 by using a Tn5 transposon mutation method. Firstly, we constructed a random insertion transposon library of 997 mutants, out of which 11 mutants showed the defective antifungal activity against <i>R. solani</i>. Furthermore, the 10 antagonism-related genes were successfully identified based on analysis of the Tn5 transposon insertion site. Indeed, this result indicated that three mutants were inserted on an indigenous plasmid in which the same insertion site was observed in two mutants. In addition, the remaining eight mutants were inserted on different genes encoding glycosyl transferase, histone H1, nonribosomal peptide synthetase, methyltransferase, MnmG, sulfate export transporter, catalase/peroxidase HPI and CysD, respectively. Compared to the wild type, the 11 mutants showed a differential effect in bacteriological characteristics such as cell growth, biofilm formation and response to H<sub>2</sub>O<sub>2</sub> stress, revealing the complexity of action mode of these antagonism-related genes. However, a significant reduction of cell motility was observed in the 11 mutants compared to the wild type. Therefore, it can be inferred that the antifungal mechanism of the 10 above-mentioned genes may be, at least partially, due to the weakness of cell motility. Overall, the result of this study will be helpful for us to understand the biocontrol mechanism of this bacterium. |
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| spelling | doaj.art-3e3042b52baf4dbc962fabe73ca5496f2023-11-20T15:20:19ZengMDPI AGPathogens2076-08172020-09-0191079710.3390/pathogens9100797Identification of Genes Involved in Antifungal Activity of <i>Burkholderia seminalis</i> Against <i>Rhizoctonia solani</i> Using Tn5 Transposon Mutation MethodMuchen Zhang0Xiaoxuan Wang1Temoor Ahmed2Mengju Liu3Zhifeng Wu4Jinyan Luo5Ye Tian6Hubiao Jiang7Yanli Wang8Guochang Sun9Bin Li10State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaDepartment of Plant Quarantine, Shanghai Extension and Service Center of Agriculture Technology, Shanghai 201103, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China<i>Rhizoctonia solani</i> is the causative agent of rice sheath blight disease. In a previous study, we found that the growth of <i>R. solani</i> was inhibited by <i>Burkholderia seminalis</i> strain R456. Therefore, the present study was conducted to identify the genes involved in the antifungal activity of <i>B. seminalis</i> strain R456 by using a Tn5 transposon mutation method. Firstly, we constructed a random insertion transposon library of 997 mutants, out of which 11 mutants showed the defective antifungal activity against <i>R. solani</i>. Furthermore, the 10 antagonism-related genes were successfully identified based on analysis of the Tn5 transposon insertion site. Indeed, this result indicated that three mutants were inserted on an indigenous plasmid in which the same insertion site was observed in two mutants. In addition, the remaining eight mutants were inserted on different genes encoding glycosyl transferase, histone H1, nonribosomal peptide synthetase, methyltransferase, MnmG, sulfate export transporter, catalase/peroxidase HPI and CysD, respectively. Compared to the wild type, the 11 mutants showed a differential effect in bacteriological characteristics such as cell growth, biofilm formation and response to H<sub>2</sub>O<sub>2</sub> stress, revealing the complexity of action mode of these antagonism-related genes. However, a significant reduction of cell motility was observed in the 11 mutants compared to the wild type. Therefore, it can be inferred that the antifungal mechanism of the 10 above-mentioned genes may be, at least partially, due to the weakness of cell motility. Overall, the result of this study will be helpful for us to understand the biocontrol mechanism of this bacterium.https://www.mdpi.com/2076-0817/9/10/797antagonismTn5<i>Burkholderia seminalis</i><i>Rhizoctonia solani</i>rice sheath blight |
| spellingShingle | Muchen Zhang Xiaoxuan Wang Temoor Ahmed Mengju Liu Zhifeng Wu Jinyan Luo Ye Tian Hubiao Jiang Yanli Wang Guochang Sun Bin Li Identification of Genes Involved in Antifungal Activity of <i>Burkholderia seminalis</i> Against <i>Rhizoctonia solani</i> Using Tn5 Transposon Mutation Method Pathogens antagonism Tn5 <i>Burkholderia seminalis</i> <i>Rhizoctonia solani</i> rice sheath blight |
| title | Identification of Genes Involved in Antifungal Activity of <i>Burkholderia seminalis</i> Against <i>Rhizoctonia solani</i> Using Tn5 Transposon Mutation Method |
| title_full | Identification of Genes Involved in Antifungal Activity of <i>Burkholderia seminalis</i> Against <i>Rhizoctonia solani</i> Using Tn5 Transposon Mutation Method |
| title_fullStr | Identification of Genes Involved in Antifungal Activity of <i>Burkholderia seminalis</i> Against <i>Rhizoctonia solani</i> Using Tn5 Transposon Mutation Method |
| title_full_unstemmed | Identification of Genes Involved in Antifungal Activity of <i>Burkholderia seminalis</i> Against <i>Rhizoctonia solani</i> Using Tn5 Transposon Mutation Method |
| title_short | Identification of Genes Involved in Antifungal Activity of <i>Burkholderia seminalis</i> Against <i>Rhizoctonia solani</i> Using Tn5 Transposon Mutation Method |
| title_sort | identification of genes involved in antifungal activity of i burkholderia seminalis i against i rhizoctonia solani i using tn5 transposon mutation method |
| topic | antagonism Tn5 <i>Burkholderia seminalis</i> <i>Rhizoctonia solani</i> rice sheath blight |
| url | https://www.mdpi.com/2076-0817/9/10/797 |
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