Promoter Methylation of Two HOXA9 and NISCH Genes in Opium Users
Background: Opiate abuse has been critically increased in the world, especially in Iran. Owing to the association of opiate use with multiple human cancers and neurological disorders, seeking for genetic and epigenetic effects of opium can pave the way for early diagnosis of major health defects in...
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| Format: | Article |
| Language: | English |
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Kerman University of Medical Sciences
2023-04-01
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| Series: | Addiction and Health |
| Subjects: | |
| Online Access: | https://ahj.kmu.ac.ir/article_92216_4f3a5d2cc8fd72f41d5142ac91eb0527.pdf |
| _version_ | 1797693678845689856 |
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| author | Majid Mahmoodi Fatemeh Karami Hamidreza Abdollahi Navidreza Giahi Kouros Divsalar Amin Honarmand Mohammad Hossein Modarressi |
| author_facet | Majid Mahmoodi Fatemeh Karami Hamidreza Abdollahi Navidreza Giahi Kouros Divsalar Amin Honarmand Mohammad Hossein Modarressi |
| author_sort | Majid Mahmoodi |
| collection | DOAJ |
| description | Background: Opiate abuse has been critically increased in the world, especially in Iran. Owing to the association of opiate use with multiple human cancers and neurological disorders, seeking for genetic and epigenetic effects of opium can pave the way for early diagnosis of major health defects in addicted users. Accordingly, the present study aimed to determine the methylation status of the promoter of two genes, which are actively involved in neurodevelopment and cancer evolution.Methods: DNA was isolated from peripheral blood of 28 opium abusers and 19 healthy controls and then subjected to sonication. Sonicated DNAs undergone methylated DNA immunoprecipitation-real time polymerase chain reaction (MeDIP-Real Time PCR) using specific primer pairs designed for HOXA9 and NISCH genes. Obtained data were analyzed using SPSS software.Findings: HOXA9 and NISCH genes were found to be significantly methylated in addicted users compared to controls (P<0.001) which was significantly associated with the mean of the age regarding HOXA9 gene (P=0.002). Neither opium amount nor duration or route of using was associated with the methylation status of HOXA9 or NISCH genes.Conclusion: Hypermethylation of HOXA9 and NISCH genes as tumor suppressor in opium-addicted individuals can be considered as confirmatory evidence for carcinogenesis of opium. Further studies are required to figure out the role of epigenetic alterations in cancer evolution among opium users. |
| first_indexed | 2024-03-12T02:47:05Z |
| format | Article |
| id | doaj.art-3e3046e537d140659e89f0c184bf1920 |
| institution | Directory Open Access Journal |
| issn | 2008-4633 2008-8469 |
| language | English |
| last_indexed | 2024-03-12T02:47:05Z |
| publishDate | 2023-04-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Addiction and Health |
| spelling | doaj.art-3e3046e537d140659e89f0c184bf19202023-09-04T06:42:15ZengKerman University of Medical SciencesAddiction and Health2008-46332008-84692023-04-01152879210.34172/ahj.2023.135692216Promoter Methylation of Two HOXA9 and NISCH Genes in Opium UsersMajid Mahmoodi0Fatemeh Karami1Hamidreza Abdollahi2Navidreza Giahi3Kouros Divsalar4Amin Honarmand5Mohammad Hossein Modarressi61. Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran 2. Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran1.Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran 2.Department of Medical Genetics, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Medical Genetics, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, IranAkhtar Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IranNeuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IranDepartment of Emergency Medicine, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, IranDepartment of Medical Genetics, Tehran University of Medical Sciences, Tehran, IranBackground: Opiate abuse has been critically increased in the world, especially in Iran. Owing to the association of opiate use with multiple human cancers and neurological disorders, seeking for genetic and epigenetic effects of opium can pave the way for early diagnosis of major health defects in addicted users. Accordingly, the present study aimed to determine the methylation status of the promoter of two genes, which are actively involved in neurodevelopment and cancer evolution.Methods: DNA was isolated from peripheral blood of 28 opium abusers and 19 healthy controls and then subjected to sonication. Sonicated DNAs undergone methylated DNA immunoprecipitation-real time polymerase chain reaction (MeDIP-Real Time PCR) using specific primer pairs designed for HOXA9 and NISCH genes. Obtained data were analyzed using SPSS software.Findings: HOXA9 and NISCH genes were found to be significantly methylated in addicted users compared to controls (P<0.001) which was significantly associated with the mean of the age regarding HOXA9 gene (P=0.002). Neither opium amount nor duration or route of using was associated with the methylation status of HOXA9 or NISCH genes.Conclusion: Hypermethylation of HOXA9 and NISCH genes as tumor suppressor in opium-addicted individuals can be considered as confirmatory evidence for carcinogenesis of opium. Further studies are required to figure out the role of epigenetic alterations in cancer evolution among opium users.https://ahj.kmu.ac.ir/article_92216_4f3a5d2cc8fd72f41d5142ac91eb0527.pdfhoxa9nischpromoter methylationopiummedip-real time pcr |
| spellingShingle | Majid Mahmoodi Fatemeh Karami Hamidreza Abdollahi Navidreza Giahi Kouros Divsalar Amin Honarmand Mohammad Hossein Modarressi Promoter Methylation of Two HOXA9 and NISCH Genes in Opium Users Addiction and Health hoxa9 nisch promoter methylation opium medip-real time pcr |
| title | Promoter Methylation of Two HOXA9 and NISCH Genes in Opium Users |
| title_full | Promoter Methylation of Two HOXA9 and NISCH Genes in Opium Users |
| title_fullStr | Promoter Methylation of Two HOXA9 and NISCH Genes in Opium Users |
| title_full_unstemmed | Promoter Methylation of Two HOXA9 and NISCH Genes in Opium Users |
| title_short | Promoter Methylation of Two HOXA9 and NISCH Genes in Opium Users |
| title_sort | promoter methylation of two hoxa9 and nisch genes in opium users |
| topic | hoxa9 nisch promoter methylation opium medip-real time pcr |
| url | https://ahj.kmu.ac.ir/article_92216_4f3a5d2cc8fd72f41d5142ac91eb0527.pdf |
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