Exogenous melatonin mediates radish (Raphanus sativus) and Alternaria brassicae interaction in a dose-dependent manner
Radish (Raphanus sativus L.) is an economically important vegetable worldwide, but its sustainable production and breeding are highly threatened by blight disease caused by Alternaria brassicae. Melatonin is an important growth regulator that can influence physiological activities in both plants and...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1126669/full |
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| author | Jingwei Li Jingwei Li Tingmin Huang Tingmin Huang Ming Xia Ming Xia Ming Xia Jinbiao Lu Jinbiao Lu Xiuhong Xu Xiuhong Xu Haiyi Liu Haiyi Liu Wanping Zhang Wanping Zhang |
| author_facet | Jingwei Li Jingwei Li Tingmin Huang Tingmin Huang Ming Xia Ming Xia Ming Xia Jinbiao Lu Jinbiao Lu Xiuhong Xu Xiuhong Xu Haiyi Liu Haiyi Liu Wanping Zhang Wanping Zhang |
| author_sort | Jingwei Li |
| collection | DOAJ |
| description | Radish (Raphanus sativus L.) is an economically important vegetable worldwide, but its sustainable production and breeding are highly threatened by blight disease caused by Alternaria brassicae. Melatonin is an important growth regulator that can influence physiological activities in both plants and microbes and stimulate biotic stress resistance in plants. In this study, 0-1500 μM melatonin was exogenously applied to healthy radish seedlings, in vitro incubated A. brassicae, and diseased radish seedlings to determine the effects of melatonin on host, pathogen, and host-pathogen interaction. At sufficient concentrations (0-500 μM), melatonin enhanced growth and immunity of healthy radish seedlings by improving the function of organelles and promoting the biosynthesis of antioxidant enzymes, chitin, organic acid, and defense proteins. Interestingly, melatonin also improved colony growth, development, and virulence of A. brassicae. A strong dosage-dependent effect of melatonin was observed: 50-500 μM promoted host and pathogen vitality and resistance (500 μM was optimal) and 1500 μM inhibited these processes. Significantly less blight was observed on diseased seedlings treated with 500 μM melatonin, indicating that melatonin more strongly enhanced the growth and immunity of radish than it promoted the development and virulence of A. brassicae at this treatment concentration. These effects of MT were mediated by transcriptional changes of key genes as identified by RNA-seq, Dual RNA-seq, and qRT-PCR. The results from this work provide a theoretical basis for the application of melatonin to protect vegetable crops against pathogens. |
| first_indexed | 2024-04-10T07:05:59Z |
| format | Article |
| id | doaj.art-3e344add85d34f9dbe85239820a4f619 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T07:05:59Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3e344add85d34f9dbe85239820a4f6192023-02-27T07:46:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.11266691126669Exogenous melatonin mediates radish (Raphanus sativus) and Alternaria brassicae interaction in a dose-dependent mannerJingwei Li0Jingwei Li1Tingmin Huang2Tingmin Huang3Ming Xia4Ming Xia5Ming Xia6Jinbiao Lu7Jinbiao Lu8Xiuhong Xu9Xiuhong Xu10Haiyi Liu11Haiyi Liu12Wanping Zhang13Wanping Zhang14Institute of Vegetable Industry Technology Research, Guizhou University, Guiyang, ChinaCollege of Agriculture, Guizhou University, Guiyang, ChinaInstitute of Vegetable Industry Technology Research, Guizhou University, Guiyang, ChinaCollege of Agriculture, Guizhou University, Guiyang, ChinaInstitute of Vegetable Industry Technology Research, Guizhou University, Guiyang, ChinaCollege of Agriculture, Guizhou University, Guiyang, ChinaSchool of Computing, Chongqing College of Humanities, Science and Technology, Hechuan, ChinaInstitute of Vegetable Industry Technology Research, Guizhou University, Guiyang, ChinaCollege of Agriculture, Guizhou University, Guiyang, ChinaInstitute of Vegetable Industry Technology Research, Guizhou University, Guiyang, ChinaCollege of Agriculture, Guizhou University, Guiyang, ChinaInstitute of Vegetable Industry Technology Research, Guizhou University, Guiyang, ChinaCollege of Agriculture, Guizhou University, Guiyang, ChinaInstitute of Vegetable Industry Technology Research, Guizhou University, Guiyang, ChinaCollege of Agriculture, Guizhou University, Guiyang, ChinaRadish (Raphanus sativus L.) is an economically important vegetable worldwide, but its sustainable production and breeding are highly threatened by blight disease caused by Alternaria brassicae. Melatonin is an important growth regulator that can influence physiological activities in both plants and microbes and stimulate biotic stress resistance in plants. In this study, 0-1500 μM melatonin was exogenously applied to healthy radish seedlings, in vitro incubated A. brassicae, and diseased radish seedlings to determine the effects of melatonin on host, pathogen, and host-pathogen interaction. At sufficient concentrations (0-500 μM), melatonin enhanced growth and immunity of healthy radish seedlings by improving the function of organelles and promoting the biosynthesis of antioxidant enzymes, chitin, organic acid, and defense proteins. Interestingly, melatonin also improved colony growth, development, and virulence of A. brassicae. A strong dosage-dependent effect of melatonin was observed: 50-500 μM promoted host and pathogen vitality and resistance (500 μM was optimal) and 1500 μM inhibited these processes. Significantly less blight was observed on diseased seedlings treated with 500 μM melatonin, indicating that melatonin more strongly enhanced the growth and immunity of radish than it promoted the development and virulence of A. brassicae at this treatment concentration. These effects of MT were mediated by transcriptional changes of key genes as identified by RNA-seq, Dual RNA-seq, and qRT-PCR. The results from this work provide a theoretical basis for the application of melatonin to protect vegetable crops against pathogens.https://www.frontiersin.org/articles/10.3389/fpls.2023.1126669/fullradishAlternaria brassicaemelatonininteractiondose-dependent manner |
| spellingShingle | Jingwei Li Jingwei Li Tingmin Huang Tingmin Huang Ming Xia Ming Xia Ming Xia Jinbiao Lu Jinbiao Lu Xiuhong Xu Xiuhong Xu Haiyi Liu Haiyi Liu Wanping Zhang Wanping Zhang Exogenous melatonin mediates radish (Raphanus sativus) and Alternaria brassicae interaction in a dose-dependent manner Frontiers in Plant Science radish Alternaria brassicae melatonin interaction dose-dependent manner |
| title | Exogenous melatonin mediates radish (Raphanus sativus) and Alternaria brassicae interaction in a dose-dependent manner |
| title_full | Exogenous melatonin mediates radish (Raphanus sativus) and Alternaria brassicae interaction in a dose-dependent manner |
| title_fullStr | Exogenous melatonin mediates radish (Raphanus sativus) and Alternaria brassicae interaction in a dose-dependent manner |
| title_full_unstemmed | Exogenous melatonin mediates radish (Raphanus sativus) and Alternaria brassicae interaction in a dose-dependent manner |
| title_short | Exogenous melatonin mediates radish (Raphanus sativus) and Alternaria brassicae interaction in a dose-dependent manner |
| title_sort | exogenous melatonin mediates radish raphanus sativus and alternaria brassicae interaction in a dose dependent manner |
| topic | radish Alternaria brassicae melatonin interaction dose-dependent manner |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1126669/full |
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