Primary characterization of the immune response in pigs infected with Trichinella spiralis
Abstract Trichinellosis, which is caused by Trichinella spiralis (T. spiralis), is a serious zoonosis. Pigs play an important role in the transmission of human trichinellosis. Characterizing the immune response to T. spiralis infection is key to elucidating host–parasite interactions. However, most...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-02-01
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| Series: | Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s13567-020-0741-0 |
| _version_ | 1818504967201226752 |
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| author | Nan Wang Xue Bai Bin Tang Yong Yang Xuelin Wang Hongfei Zhu Xuenong Luo Hongbin Yan Hong Jia Mingyuan Liu Xiaolei Liu |
| author_facet | Nan Wang Xue Bai Bin Tang Yong Yang Xuelin Wang Hongfei Zhu Xuenong Luo Hongbin Yan Hong Jia Mingyuan Liu Xiaolei Liu |
| author_sort | Nan Wang |
| collection | DOAJ |
| description | Abstract Trichinellosis, which is caused by Trichinella spiralis (T. spiralis), is a serious zoonosis. Pigs play an important role in the transmission of human trichinellosis. Characterizing the immune response to T. spiralis infection is key to elucidating host–parasite interactions. However, most studies on the immune response to T. spiralis infection have employed murine models. In this study, we investigated the immune response to T. spiralis infection in pigs. The results showed that the average numbers of larvae per gram (lpg) for the 100-muscle larvae (ML), 1000-ML, and 10 000-ML groups were 1.502, 35.947, and 398.811, respectively. The percentages of CD3+ T cells, B cells, CD4+ T cells, Treg cells, and Th17 cells were elevated in the infection groups compared to the control animals. In contrast, CD8+ T cell percentages were reduced after infection in the low-dose group. The number of neutrophils was increased at 3–17 days post-infection (dpi). Th1 cytokine IL-2 levels were significantly decreased at 7 dpi, and Th2 cytokine IL-4 levels were significantly elevated at 3 dpi. Treg cytokine IL-10 levels were significantly elevated between 7 dpi and 30 dpi. Th17 cytokine IL-17A levels were significantly increased beginning at 11 dpi. These results confirmed that pigs infected with T. spiralis predominantly induced Th2 and Treg immune responses, which suppress the Th1 immune responses. This study provides novel insights into the immune response of pigs infected with T. spiralis. |
| first_indexed | 2024-12-10T21:44:22Z |
| format | Article |
| id | doaj.art-3e354d6fec044e68853e76ec7b4b396b |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-10T21:44:22Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-3e354d6fec044e68853e76ec7b4b396b2022-12-22T01:32:24ZengBMCVeterinary Research1297-97162020-02-0151111410.1186/s13567-020-0741-0Primary characterization of the immune response in pigs infected with Trichinella spiralisNan Wang0Xue Bai1Bin Tang2Yong Yang3Xuelin Wang4Hongfei Zhu5Xuenong Luo6Hongbin Yan7Hong Jia8Mingyuan Liu9Xiaolei Liu10Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin UniversityInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin UniversityAbstract Trichinellosis, which is caused by Trichinella spiralis (T. spiralis), is a serious zoonosis. Pigs play an important role in the transmission of human trichinellosis. Characterizing the immune response to T. spiralis infection is key to elucidating host–parasite interactions. However, most studies on the immune response to T. spiralis infection have employed murine models. In this study, we investigated the immune response to T. spiralis infection in pigs. The results showed that the average numbers of larvae per gram (lpg) for the 100-muscle larvae (ML), 1000-ML, and 10 000-ML groups were 1.502, 35.947, and 398.811, respectively. The percentages of CD3+ T cells, B cells, CD4+ T cells, Treg cells, and Th17 cells were elevated in the infection groups compared to the control animals. In contrast, CD8+ T cell percentages were reduced after infection in the low-dose group. The number of neutrophils was increased at 3–17 days post-infection (dpi). Th1 cytokine IL-2 levels were significantly decreased at 7 dpi, and Th2 cytokine IL-4 levels were significantly elevated at 3 dpi. Treg cytokine IL-10 levels were significantly elevated between 7 dpi and 30 dpi. Th17 cytokine IL-17A levels were significantly increased beginning at 11 dpi. These results confirmed that pigs infected with T. spiralis predominantly induced Th2 and Treg immune responses, which suppress the Th1 immune responses. This study provides novel insights into the immune response of pigs infected with T. spiralis.http://link.springer.com/article/10.1186/s13567-020-0741-0 |
| spellingShingle | Nan Wang Xue Bai Bin Tang Yong Yang Xuelin Wang Hongfei Zhu Xuenong Luo Hongbin Yan Hong Jia Mingyuan Liu Xiaolei Liu Primary characterization of the immune response in pigs infected with Trichinella spiralis Veterinary Research |
| title | Primary characterization of the immune response in pigs infected with Trichinella spiralis |
| title_full | Primary characterization of the immune response in pigs infected with Trichinella spiralis |
| title_fullStr | Primary characterization of the immune response in pigs infected with Trichinella spiralis |
| title_full_unstemmed | Primary characterization of the immune response in pigs infected with Trichinella spiralis |
| title_short | Primary characterization of the immune response in pigs infected with Trichinella spiralis |
| title_sort | primary characterization of the immune response in pigs infected with trichinella spiralis |
| url | http://link.springer.com/article/10.1186/s13567-020-0741-0 |
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