Electromagnetic fields at 2.45 GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland
Summary Non-ionizing radiation at 2.45 GHz may modify the expression of genes that codify heat shock proteins (HSP) in the thyroid gland. Using the enzyme-linked immunosorbent assay (ELISA) technique, we studied levels of HSP-90 and HSP-70. We also used hematoxilin eosin to look for evidence of lesi...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2012-07-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/1/9/831 |
| _version_ | 1818901001712697344 |
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| author | María José Misa Agustiño José Manuel Leiro María Teresa Jorge Mora Juan Antonio Rodríguez-González Francisco Javier Jorge Barreiro Francisco José Ares-Pena Elena López-Martín |
| author_facet | María José Misa Agustiño José Manuel Leiro María Teresa Jorge Mora Juan Antonio Rodríguez-González Francisco Javier Jorge Barreiro Francisco José Ares-Pena Elena López-Martín |
| author_sort | María José Misa Agustiño |
| collection | DOAJ |
| description | Summary
Non-ionizing radiation at 2.45 GHz may modify the expression of genes that codify heat shock proteins (HSP) in the thyroid gland. Using the enzyme-linked immunosorbent assay (ELISA) technique, we studied levels of HSP-90 and HSP-70. We also used hematoxilin eosin to look for evidence of lesions in the gland and applied the DAPI technique of fluorescence to search for evidence of chromatin condensation and nuclear fragmentation in the thyroid cells of adult female Sprague-Dawley rats. Fifty-four rats were individually exposed for 30 min to 2.45 GHz radiation in a Gigahertz transverse electromagnetic (GTEM) cell at different levels of non-thermal specific absorption rate (SAR), which was calculated using the finite difference time domain (FDTD) technique. Ninety minutes after radiation, HSP-90 and HSP-70 had decreased significantly (P<0.01) after applying a SAR of 0.046±1.10 W/Kg or 0.104±5.10−3 W/Kg. Twenty-four hours after radiation, HSP-90 had partially recovered and HSP-70 had recovered completely. There were few indications of lesions in the glandular structure and signs of apoptosis were negative in all radiated animals. The results suggest that acute sub-thermal radiation at 2.45 GHz may alter levels of cellular stress in rat thyroid gland without initially altering their anti-apoptotic capacity. |
| first_indexed | 2024-12-19T20:12:49Z |
| format | Article |
| id | doaj.art-3e35c33415fc4380a13b000804395c2a |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-19T20:12:49Z |
| publishDate | 2012-07-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-3e35c33415fc4380a13b000804395c2a2022-12-21T20:07:16ZengThe Company of BiologistsBiology Open2046-63902012-07-011983183810.1242/bio.2012129720121297Electromagnetic fields at 2.45 GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid glandMaría José Misa Agustiño0José Manuel Leiro1María Teresa Jorge Mora2Juan Antonio Rodríguez-González3Francisco Javier Jorge Barreiro4Francisco José Ares-Pena5Elena López-Martín6 Morphological Sciences Department Institute of Food Research and Analysis Morphological Sciences Department Applied Physics Department, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain Morphological Sciences Department Applied Physics Department, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain Morphological Sciences Department Summary Non-ionizing radiation at 2.45 GHz may modify the expression of genes that codify heat shock proteins (HSP) in the thyroid gland. Using the enzyme-linked immunosorbent assay (ELISA) technique, we studied levels of HSP-90 and HSP-70. We also used hematoxilin eosin to look for evidence of lesions in the gland and applied the DAPI technique of fluorescence to search for evidence of chromatin condensation and nuclear fragmentation in the thyroid cells of adult female Sprague-Dawley rats. Fifty-four rats were individually exposed for 30 min to 2.45 GHz radiation in a Gigahertz transverse electromagnetic (GTEM) cell at different levels of non-thermal specific absorption rate (SAR), which was calculated using the finite difference time domain (FDTD) technique. Ninety minutes after radiation, HSP-90 and HSP-70 had decreased significantly (P<0.01) after applying a SAR of 0.046±1.10 W/Kg or 0.104±5.10−3 W/Kg. Twenty-four hours after radiation, HSP-90 had partially recovered and HSP-70 had recovered completely. There were few indications of lesions in the glandular structure and signs of apoptosis were negative in all radiated animals. The results suggest that acute sub-thermal radiation at 2.45 GHz may alter levels of cellular stress in rat thyroid gland without initially altering their anti-apoptotic capacity.http://bio.biologists.org/content/1/9/831Apoptotic activityElectromagnetic fieldsShock proteinsThyroid gland |
| spellingShingle | María José Misa Agustiño José Manuel Leiro María Teresa Jorge Mora Juan Antonio Rodríguez-González Francisco Javier Jorge Barreiro Francisco José Ares-Pena Elena López-Martín Electromagnetic fields at 2.45 GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland Biology Open Apoptotic activity Electromagnetic fields Shock proteins Thyroid gland |
| title | Electromagnetic fields at 2.45 GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland |
| title_full | Electromagnetic fields at 2.45 GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland |
| title_fullStr | Electromagnetic fields at 2.45 GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland |
| title_full_unstemmed | Electromagnetic fields at 2.45 GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland |
| title_short | Electromagnetic fields at 2.45 GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland |
| title_sort | electromagnetic fields at 2 45 ghz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland |
| topic | Apoptotic activity Electromagnetic fields Shock proteins Thyroid gland |
| url | http://bio.biologists.org/content/1/9/831 |
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