Effects of Resistant Dextrin from Potato Starch on the Growth Dynamics of Selected Co-Cultured Strains of Gastrointestinal Bacteria and the Activity of Fecal Enzymes
Preparations of resistant dextrins have become an interesting topic of research due to their properties, which bear resemblance those of prebiotics, e.g., the improvement of metabolic parameters, increased efficiency of the immune system and induction of vitamin production. The aim of this study was...
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MDPI AG
2022-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/10/2158 |
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| author | Michał Włodarczyk Katarzyna Śliżewska Renata Barczyńska Janusz Kapuśniak |
| author_facet | Michał Włodarczyk Katarzyna Śliżewska Renata Barczyńska Janusz Kapuśniak |
| author_sort | Michał Włodarczyk |
| collection | DOAJ |
| description | Preparations of resistant dextrins have become an interesting topic of research due to their properties, which bear resemblance those of prebiotics, e.g., the improvement of metabolic parameters, increased efficiency of the immune system and induction of vitamin production. The aim of this study was to investigate the effects of the resistant dextrin produced from potato starch on the growth dynamics of typical gastrointestinal microbiota and the activity of fecal enzymes in order to assess a possible exhibition of prebiotic properties. In the study, in vitro cultivation of co-cultures of <i>Lactobacillus</i>, <i>Bifidobacterium</i>, <i>E. coli</i>, <i>Enterococcus</i>, <i>Clostridium</i> and <i>Bacteroides</i> spp. was conducted on media enriched with the resistant dextrin. The CFU/mL for each strain was measured in time periods of 24, 48, 72, 96 and 168 h. Furthermore, the activities of α-glucosidase, α-galactosidase, β-glucosidase, β-galactosidase and β-glucuronidase were determined using spectrophotometric methods at a wavelength of 400 nm. The results show that the resistant dextrin can be utilized as a source of carbon for the growth of intestinal bacteria. Moreover, the results revealed that, after 168 h of cultivation, it enhances the viability of probiotic strains of <i>Lactobacillus</i> and <i>Bifidobacterium</i> spp. and decreases the growth of other intestinal strains (<i>Clostridium</i>, <i>Escherichia coli</i>, <i>Enterococcus</i> and <i>Bacteroides</i>), which is demonstrated by a high Prebiotic Index (<i>p</i> < 0.05). Furthermore, there was no significant change in the pH of the cultures; however, the pace of the pH decrease during the cultivation was slower in the case of culture with resistant dextrin. Furthermore, it was revealed that usage of the resistant dextrin as a medium additive noticeably lowered the activities of β-glucosidase and β-glucuronidase compared to the control (<i>p</i> < 0.05), whereas the activities of the other fecal enzymes were affected to a lesser degree. The resistant dextrins derived from potato starch are a suitable prebiotic candidate as they promote the growth of beneficial strains of gut bacteria and improve health markers, such as the activity of fecal enzymes. Nevertheless, additional in vivo research is necessary to further assess the suspected health-promoting properties. |
| first_indexed | 2024-03-10T03:13:13Z |
| format | Article |
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| language | English |
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| spelling | doaj.art-3e35fc3974034ff3913614d7f30272482023-11-23T12:30:59ZengMDPI AGNutrients2072-66432022-05-011410215810.3390/nu14102158Effects of Resistant Dextrin from Potato Starch on the Growth Dynamics of Selected Co-Cultured Strains of Gastrointestinal Bacteria and the Activity of Fecal EnzymesMichał Włodarczyk0Katarzyna Śliżewska1Renata Barczyńska2Janusz Kapuśniak3Department of Biotechnology and Food Sciences, Institute of Fermentation Technology and Microbiology, Technical University of Lodz, Wolczanska 171/173, 90-924 Łódź, PolandDepartment of Biotechnology and Food Sciences, Institute of Fermentation Technology and Microbiology, Technical University of Lodz, Wolczanska 171/173, 90-924 Łódź, PolandDepartment of Dietetics and Food Studies, Faculty of Science and Technology, Jan Dlugosz University, Armii Krajowej 13/15, 42-200 Czestochowa, PolandDepartment of Dietetics and Food Studies, Faculty of Science and Technology, Jan Dlugosz University, Armii Krajowej 13/15, 42-200 Czestochowa, PolandPreparations of resistant dextrins have become an interesting topic of research due to their properties, which bear resemblance those of prebiotics, e.g., the improvement of metabolic parameters, increased efficiency of the immune system and induction of vitamin production. The aim of this study was to investigate the effects of the resistant dextrin produced from potato starch on the growth dynamics of typical gastrointestinal microbiota and the activity of fecal enzymes in order to assess a possible exhibition of prebiotic properties. In the study, in vitro cultivation of co-cultures of <i>Lactobacillus</i>, <i>Bifidobacterium</i>, <i>E. coli</i>, <i>Enterococcus</i>, <i>Clostridium</i> and <i>Bacteroides</i> spp. was conducted on media enriched with the resistant dextrin. The CFU/mL for each strain was measured in time periods of 24, 48, 72, 96 and 168 h. Furthermore, the activities of α-glucosidase, α-galactosidase, β-glucosidase, β-galactosidase and β-glucuronidase were determined using spectrophotometric methods at a wavelength of 400 nm. The results show that the resistant dextrin can be utilized as a source of carbon for the growth of intestinal bacteria. Moreover, the results revealed that, after 168 h of cultivation, it enhances the viability of probiotic strains of <i>Lactobacillus</i> and <i>Bifidobacterium</i> spp. and decreases the growth of other intestinal strains (<i>Clostridium</i>, <i>Escherichia coli</i>, <i>Enterococcus</i> and <i>Bacteroides</i>), which is demonstrated by a high Prebiotic Index (<i>p</i> < 0.05). Furthermore, there was no significant change in the pH of the cultures; however, the pace of the pH decrease during the cultivation was slower in the case of culture with resistant dextrin. Furthermore, it was revealed that usage of the resistant dextrin as a medium additive noticeably lowered the activities of β-glucosidase and β-glucuronidase compared to the control (<i>p</i> < 0.05), whereas the activities of the other fecal enzymes were affected to a lesser degree. The resistant dextrins derived from potato starch are a suitable prebiotic candidate as they promote the growth of beneficial strains of gut bacteria and improve health markers, such as the activity of fecal enzymes. Nevertheless, additional in vivo research is necessary to further assess the suspected health-promoting properties.https://www.mdpi.com/2072-6643/14/10/2158resistant dextrinprebioticgut microbiotabacterial enzymes |
| spellingShingle | Michał Włodarczyk Katarzyna Śliżewska Renata Barczyńska Janusz Kapuśniak Effects of Resistant Dextrin from Potato Starch on the Growth Dynamics of Selected Co-Cultured Strains of Gastrointestinal Bacteria and the Activity of Fecal Enzymes Nutrients resistant dextrin prebiotic gut microbiota bacterial enzymes |
| title | Effects of Resistant Dextrin from Potato Starch on the Growth Dynamics of Selected Co-Cultured Strains of Gastrointestinal Bacteria and the Activity of Fecal Enzymes |
| title_full | Effects of Resistant Dextrin from Potato Starch on the Growth Dynamics of Selected Co-Cultured Strains of Gastrointestinal Bacteria and the Activity of Fecal Enzymes |
| title_fullStr | Effects of Resistant Dextrin from Potato Starch on the Growth Dynamics of Selected Co-Cultured Strains of Gastrointestinal Bacteria and the Activity of Fecal Enzymes |
| title_full_unstemmed | Effects of Resistant Dextrin from Potato Starch on the Growth Dynamics of Selected Co-Cultured Strains of Gastrointestinal Bacteria and the Activity of Fecal Enzymes |
| title_short | Effects of Resistant Dextrin from Potato Starch on the Growth Dynamics of Selected Co-Cultured Strains of Gastrointestinal Bacteria and the Activity of Fecal Enzymes |
| title_sort | effects of resistant dextrin from potato starch on the growth dynamics of selected co cultured strains of gastrointestinal bacteria and the activity of fecal enzymes |
| topic | resistant dextrin prebiotic gut microbiota bacterial enzymes |
| url | https://www.mdpi.com/2072-6643/14/10/2158 |
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