Antioxidant effects of adipose derived stem cells conditioned media against high glucose-induced injuries in cultured of C28I2 chondrocytes
Background: Osteoarthritis (OA) is the most common form of arthritis characterized by progressive loss of articular cartilage, causing pain and loss of articular function. High glucose is a crucial inflammatory factor playing a pivotal role in the pathogenesis of OA that induces ROS production. Sinc...
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2023-04-01
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| Series: | Tehran University Medical Journal |
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| Online Access: | http://tumj.tums.ac.ir/article-1-12328-en.pdf |
| _version_ | 1797742443115839488 |
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| author | Sedigheh Safari Akram Eidi Mehrnaz Mehrabani Mohammad Javad Fatemi Ali Mohammad Sharifi |
| author_facet | Sedigheh Safari Akram Eidi Mehrnaz Mehrabani Mohammad Javad Fatemi Ali Mohammad Sharifi |
| author_sort | Sedigheh Safari |
| collection | DOAJ |
| description | Background: Osteoarthritis (OA) is the most common form of arthritis characterized by progressive loss of articular cartilage, causing pain and loss of articular function. High glucose is a crucial inflammatory factor playing a pivotal role in the pathogenesis of OA that induces ROS production. Since most of the current therapies for OA are short-term benefits, hence, there is high demand for finding novel therapeutic agents for OA treatment. Recent studies have demonstrated that mesenchymal stem cells secrete important therapeutic factors that protect chondrocytes. In the current study, we investigated the protective potential of Adipose-derived stem cell conditioned medium (CM-ADSC) as an alternative to cell therapy in high glucose-mediated oxidative stress in C28I2 human chondrocytes.
Methods: This experimental study was performed in the Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran from May 2018 to August 2020. Adipose-derived stem cells were cultured until they reached 90% confluence then washed with PBS and cultured in a FBS-free medium for 48 hours. The conditioned medium was collected and centrifuged. The protective effect of the concentration of conditioned medium on high glucose (75mM)-induced oxidative stress in C28I2 cell viability was evaluated by WST-1 assay. Total RNA was isolated from the treated and untreated cells with TRIzol reagent. The mRNA expression of antioxidant enzymes including, glutathione S-transferase-P1 (GSTP1), catalase (CAT), and superoxide dismutase1 (SOD1) was evaluated by reverse transcription-polymerase chain reaction in treatment and non-treatment groups.
Results: Adipose-derived stem cell conditioned medium pretreatment remarkably protected C28I2 cells against high glucose. The expression of mRNA of CAT, GSTP1, and SOD1 significantly increased following treatment with the conditioned medium (50%) for 24 hours in high glucose-exposed cells as compared to the control.
Conclusion: Present study indicates that the Adipose-derived stem cell conditioned medium can reduce oxidative stress. It seems that the conditioned medium may protect cartilage in the progression of osteoarthritis. |
| first_indexed | 2024-03-12T14:40:54Z |
| format | Article |
| id | doaj.art-3e3973958c8f41c7b10335428e9c4c80 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-03-12T14:40:54Z |
| publishDate | 2023-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-3e3973958c8f41c7b10335428e9c4c802023-08-16T06:56:49ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222023-04-018112128Antioxidant effects of adipose derived stem cells conditioned media against high glucose-induced injuries in cultured of C28I2 chondrocytesSedigheh Safari0Akram Eidi1Mehrnaz Mehrabani2Mohammad Javad Fatemi3Ali Mohammad Sharifi4 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran. Burn Research Center, Iran University of Medical Sciences, Tehran, Iran. Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran | Tissue Engineering Group, (NOCERAL), Department of Orthopedics Surgery, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia., Tehran, Iran. Background: Osteoarthritis (OA) is the most common form of arthritis characterized by progressive loss of articular cartilage, causing pain and loss of articular function. High glucose is a crucial inflammatory factor playing a pivotal role in the pathogenesis of OA that induces ROS production. Since most of the current therapies for OA are short-term benefits, hence, there is high demand for finding novel therapeutic agents for OA treatment. Recent studies have demonstrated that mesenchymal stem cells secrete important therapeutic factors that protect chondrocytes. In the current study, we investigated the protective potential of Adipose-derived stem cell conditioned medium (CM-ADSC) as an alternative to cell therapy in high glucose-mediated oxidative stress in C28I2 human chondrocytes. Methods: This experimental study was performed in the Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran from May 2018 to August 2020. Adipose-derived stem cells were cultured until they reached 90% confluence then washed with PBS and cultured in a FBS-free medium for 48 hours. The conditioned medium was collected and centrifuged. The protective effect of the concentration of conditioned medium on high glucose (75mM)-induced oxidative stress in C28I2 cell viability was evaluated by WST-1 assay. Total RNA was isolated from the treated and untreated cells with TRIzol reagent. The mRNA expression of antioxidant enzymes including, glutathione S-transferase-P1 (GSTP1), catalase (CAT), and superoxide dismutase1 (SOD1) was evaluated by reverse transcription-polymerase chain reaction in treatment and non-treatment groups. Results: Adipose-derived stem cell conditioned medium pretreatment remarkably protected C28I2 cells against high glucose. The expression of mRNA of CAT, GSTP1, and SOD1 significantly increased following treatment with the conditioned medium (50%) for 24 hours in high glucose-exposed cells as compared to the control. Conclusion: Present study indicates that the Adipose-derived stem cell conditioned medium can reduce oxidative stress. It seems that the conditioned medium may protect cartilage in the progression of osteoarthritis.http://tumj.tums.ac.ir/article-1-12328-en.pdfconditioned mediummesenchymal stem cellosteoarthritisoxidative stress. |
| spellingShingle | Sedigheh Safari Akram Eidi Mehrnaz Mehrabani Mohammad Javad Fatemi Ali Mohammad Sharifi Antioxidant effects of adipose derived stem cells conditioned media against high glucose-induced injuries in cultured of C28I2 chondrocytes Tehran University Medical Journal conditioned medium mesenchymal stem cell osteoarthritis oxidative stress. |
| title | Antioxidant effects of adipose derived stem cells conditioned media against high glucose-induced injuries in cultured of C28I2 chondrocytes |
| title_full | Antioxidant effects of adipose derived stem cells conditioned media against high glucose-induced injuries in cultured of C28I2 chondrocytes |
| title_fullStr | Antioxidant effects of adipose derived stem cells conditioned media against high glucose-induced injuries in cultured of C28I2 chondrocytes |
| title_full_unstemmed | Antioxidant effects of adipose derived stem cells conditioned media against high glucose-induced injuries in cultured of C28I2 chondrocytes |
| title_short | Antioxidant effects of adipose derived stem cells conditioned media against high glucose-induced injuries in cultured of C28I2 chondrocytes |
| title_sort | antioxidant effects of adipose derived stem cells conditioned media against high glucose induced injuries in cultured of c28i2 chondrocytes |
| topic | conditioned medium mesenchymal stem cell osteoarthritis oxidative stress. |
| url | http://tumj.tums.ac.ir/article-1-12328-en.pdf |
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