Diversity of Pol IV function is defined by mutations at the maize rmr7 locus.
Mutations affecting the heritable maintenance of epigenetic states in maize identify multiple small RNA biogenesis factors including NRPD1, the largest subunit of the presumed maize Pol IV holoenzyme. Here we show that mutations defining the required to maintain repression7 locus identify a second R...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-11-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC2775721?pdf=render |
| _version_ | 1817997473459732480 |
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| author | Jennifer L Stonaker Jana P Lim Karl F Erhard Jay B Hollick |
| author_facet | Jennifer L Stonaker Jana P Lim Karl F Erhard Jay B Hollick |
| author_sort | Jennifer L Stonaker |
| collection | DOAJ |
| description | Mutations affecting the heritable maintenance of epigenetic states in maize identify multiple small RNA biogenesis factors including NRPD1, the largest subunit of the presumed maize Pol IV holoenzyme. Here we show that mutations defining the required to maintain repression7 locus identify a second RNA polymerase subunit related to Arabidopsis NRPD2a, the sole second largest subunit shared between Arabidopsis Pol IV and Pol V. A phylogenetic analysis shows that, in contrast to representative eudicots, grasses have retained duplicate loci capable of producing functional NRPD2-like proteins, which is indicative of increased RNA polymerase diversity in grasses relative to eudicots. Together with comparisons of rmr7 mutant plant phenotypes and their effects on the maintenance of epigenetic states with parallel analyses of NRPD1 defects, our results imply that maize utilizes multiple functional NRPD2-like proteins. Despite the observation that RMR7/NRPD2, like NRPD1, is required for the accumulation of most siRNAs, our data indicate that different Pol IV isoforms play distinct roles in the maintenance of meiotically-heritable epigenetic information in the grasses. |
| first_indexed | 2024-04-14T02:38:49Z |
| format | Article |
| id | doaj.art-3e3e9753ac5c4b6bb676c8a5367a5015 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-14T02:38:49Z |
| publishDate | 2009-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-3e3e9753ac5c4b6bb676c8a5367a50152022-12-22T02:17:16ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042009-11-01511e100070610.1371/journal.pgen.1000706Diversity of Pol IV function is defined by mutations at the maize rmr7 locus.Jennifer L StonakerJana P LimKarl F ErhardJay B HollickMutations affecting the heritable maintenance of epigenetic states in maize identify multiple small RNA biogenesis factors including NRPD1, the largest subunit of the presumed maize Pol IV holoenzyme. Here we show that mutations defining the required to maintain repression7 locus identify a second RNA polymerase subunit related to Arabidopsis NRPD2a, the sole second largest subunit shared between Arabidopsis Pol IV and Pol V. A phylogenetic analysis shows that, in contrast to representative eudicots, grasses have retained duplicate loci capable of producing functional NRPD2-like proteins, which is indicative of increased RNA polymerase diversity in grasses relative to eudicots. Together with comparisons of rmr7 mutant plant phenotypes and their effects on the maintenance of epigenetic states with parallel analyses of NRPD1 defects, our results imply that maize utilizes multiple functional NRPD2-like proteins. Despite the observation that RMR7/NRPD2, like NRPD1, is required for the accumulation of most siRNAs, our data indicate that different Pol IV isoforms play distinct roles in the maintenance of meiotically-heritable epigenetic information in the grasses.http://europepmc.org/articles/PMC2775721?pdf=render |
| spellingShingle | Jennifer L Stonaker Jana P Lim Karl F Erhard Jay B Hollick Diversity of Pol IV function is defined by mutations at the maize rmr7 locus. PLoS Genetics |
| title | Diversity of Pol IV function is defined by mutations at the maize rmr7 locus. |
| title_full | Diversity of Pol IV function is defined by mutations at the maize rmr7 locus. |
| title_fullStr | Diversity of Pol IV function is defined by mutations at the maize rmr7 locus. |
| title_full_unstemmed | Diversity of Pol IV function is defined by mutations at the maize rmr7 locus. |
| title_short | Diversity of Pol IV function is defined by mutations at the maize rmr7 locus. |
| title_sort | diversity of pol iv function is defined by mutations at the maize rmr7 locus |
| url | http://europepmc.org/articles/PMC2775721?pdf=render |
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