Determination and quantification of the <i>in vitro</i> activity of <i>Aloe marlothii</i> (A. Berger) subsp. <i>marlothii</i> and <i>Elephantorrhiza elephantina</i> (Burch.) skeels acetone extracts against <i>Ehrlichia ruminantium</i>
An Ehrlichia ruminantium culture system was utilized for the anti-rickettsial evaluation of two ethnoveterinary plants, Elephantorrhiza elephantina and Aloe marlothii. Well-established E. ruminantium cultures were incubated with the plant leaf acetone extracts and compared to oxytetracycline and...
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| Format: | Article |
| Language: | English |
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AOSIS
2006-09-01
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| Series: | Onderstepoort Journal of Veterinary Research |
| Online Access: | https://ojvr.org/index.php/ojvr/article/view/143 |
| _version_ | 1828808713156165632 |
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| author | V. Naidoo E. Zweygarth G.E. Swan |
| author_facet | V. Naidoo E. Zweygarth G.E. Swan |
| author_sort | V. Naidoo |
| collection | DOAJ |
| description | An Ehrlichia ruminantium culture system was utilized for the anti-rickettsial evaluation of two ethnoveterinary plants, Elephantorrhiza elephantina and Aloe marlothii. Well-established E. ruminantium cultures were incubated with the plant leaf acetone extracts and compared to oxytetracycline and untreated controls. Effectivity was established by comparing the percentage parasitised cells and the calculation of both EC50 and extrapolated EC90 in µg/ml. The plant extracts were also screened for antibacterial activity using bioautography. Elephantorrhiza elephantina and A. marlothii demonstrated anti-ehrlichial activity with an EC50 of 111.4 and 64.5 µg/ml and EC 90 of 228.9 and 129.9 µg/ml, respectively. The corresponding EC50 and EC90 for oxytetracycline was 0.29 and 0.08 µg/ml. Both plants appeared to produce their inhibitory activity by a similar mechanism, unrelated to that of the tetracyclines. Both the plant acetone extracts demonstrated antibacterial activity against Escherichia coli and Staphylococcus aureus (ATCC strains). |
| first_indexed | 2024-12-12T08:44:53Z |
| format | Article |
| id | doaj.art-3e3f88f01fe14000a74b0d78c3b15771 |
| institution | Directory Open Access Journal |
| issn | 0030-2465 2219-0635 |
| language | English |
| last_indexed | 2024-12-12T08:44:53Z |
| publishDate | 2006-09-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Onderstepoort Journal of Veterinary Research |
| spelling | doaj.art-3e3f88f01fe14000a74b0d78c3b157712022-12-22T00:30:33ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352006-09-0173317517810.4102/ojvr.v73i3.143135Determination and quantification of the <i>in vitro</i> activity of <i>Aloe marlothii</i> (A. Berger) subsp. <i>marlothii</i> and <i>Elephantorrhiza elephantina</i> (Burch.) skeels acetone extracts against <i>Ehrlichia ruminantium</i>V. NaidooE. ZweygarthG.E. SwanAn Ehrlichia ruminantium culture system was utilized for the anti-rickettsial evaluation of two ethnoveterinary plants, Elephantorrhiza elephantina and Aloe marlothii. Well-established E. ruminantium cultures were incubated with the plant leaf acetone extracts and compared to oxytetracycline and untreated controls. Effectivity was established by comparing the percentage parasitised cells and the calculation of both EC50 and extrapolated EC90 in µg/ml. The plant extracts were also screened for antibacterial activity using bioautography. Elephantorrhiza elephantina and A. marlothii demonstrated anti-ehrlichial activity with an EC50 of 111.4 and 64.5 µg/ml and EC 90 of 228.9 and 129.9 µg/ml, respectively. The corresponding EC50 and EC90 for oxytetracycline was 0.29 and 0.08 µg/ml. Both plants appeared to produce their inhibitory activity by a similar mechanism, unrelated to that of the tetracyclines. Both the plant acetone extracts demonstrated antibacterial activity against Escherichia coli and Staphylococcus aureus (ATCC strains).https://ojvr.org/index.php/ojvr/article/view/143 |
| spellingShingle | V. Naidoo E. Zweygarth G.E. Swan Determination and quantification of the <i>in vitro</i> activity of <i>Aloe marlothii</i> (A. Berger) subsp. <i>marlothii</i> and <i>Elephantorrhiza elephantina</i> (Burch.) skeels acetone extracts against <i>Ehrlichia ruminantium</i> Onderstepoort Journal of Veterinary Research |
| title | Determination and quantification of the <i>in vitro</i> activity of <i>Aloe marlothii</i> (A. Berger) subsp. <i>marlothii</i> and <i>Elephantorrhiza elephantina</i> (Burch.) skeels acetone extracts against <i>Ehrlichia ruminantium</i> |
| title_full | Determination and quantification of the <i>in vitro</i> activity of <i>Aloe marlothii</i> (A. Berger) subsp. <i>marlothii</i> and <i>Elephantorrhiza elephantina</i> (Burch.) skeels acetone extracts against <i>Ehrlichia ruminantium</i> |
| title_fullStr | Determination and quantification of the <i>in vitro</i> activity of <i>Aloe marlothii</i> (A. Berger) subsp. <i>marlothii</i> and <i>Elephantorrhiza elephantina</i> (Burch.) skeels acetone extracts against <i>Ehrlichia ruminantium</i> |
| title_full_unstemmed | Determination and quantification of the <i>in vitro</i> activity of <i>Aloe marlothii</i> (A. Berger) subsp. <i>marlothii</i> and <i>Elephantorrhiza elephantina</i> (Burch.) skeels acetone extracts against <i>Ehrlichia ruminantium</i> |
| title_short | Determination and quantification of the <i>in vitro</i> activity of <i>Aloe marlothii</i> (A. Berger) subsp. <i>marlothii</i> and <i>Elephantorrhiza elephantina</i> (Burch.) skeels acetone extracts against <i>Ehrlichia ruminantium</i> |
| title_sort | determination and quantification of the i in vitro i activity of i aloe marlothii i a berger subsp i marlothii i and i elephantorrhiza elephantina i burch skeels acetone extracts against i ehrlichia ruminantium i |
| url | https://ojvr.org/index.php/ojvr/article/view/143 |
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