A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution

A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution

Summary: Innate lymphoid cells (ILCs) and adaptive T cells remain a challenge to study because of a significant overlap in their transcriptomic profiles. Here, we describe the adoptive transfer of ILC progenitors into mice genetically deficient in innate and adaptive immune cells to allow detailed s...

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Bibliographic Details
Main Authors: Nicolas Jacquelot, Qiutong Huang, Gabrielle T. Belz, Cyril Seillet
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Flow Cytometry/Mass Cytometry
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004142

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