Identification and Structural Analysis of Spirostanol Saponin from <i>Yucca schidigera</i> by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis
<i>Yucca schidigera</i> Roezl (Mojave), a kind of ornamental plant belonging to the <i>Yucca</i> genus (Agavaceae), whose extract exhibits important roles in food, beverage, cosmetic and feed additives owing to its rich spirostanol saponins. To provide a comprehensive chemica...
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MDPI AG
2020-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/17/3848 |
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| author | Jingya Ruan Lu Qu Wei Zhao Chang Gao Peijian Huang Dandan Zheng Lifeng Han Haiyang Yu Zixin Zhang Yi Zhang Tao Wang |
| author_facet | Jingya Ruan Lu Qu Wei Zhao Chang Gao Peijian Huang Dandan Zheng Lifeng Han Haiyang Yu Zixin Zhang Yi Zhang Tao Wang |
| author_sort | Jingya Ruan |
| collection | DOAJ |
| description | <i>Yucca schidigera</i> Roezl (Mojave), a kind of ornamental plant belonging to the <i>Yucca</i> genus (Agavaceae), whose extract exhibits important roles in food, beverage, cosmetic and feed additives owing to its rich spirostanol saponins. To provide a comprehensive chemical profiling of the spirostanol saponins in it, this study was performed by using a multi-phase liquid chromatography method combining a reversed phase chromatography T<sub>3</sub> column with a normal phase chromatography silica column for the separation and an ESI-Q-Exactive-Orbitrap MS in positive ion mode as the detector. By comparing the retention time and ion fragments with standards, thirty-one spirostanol saponins were identified. In addition, according to the summary of the chromatographic retention behaviors and the MS/MS cleavage patterns and biosynthetic pathway, another seventy-nine spirostanol saponins were speculatively identified, forty ones of which were potentially new ones. Moreover, ten novel spirostanol saponins (three pairs of (25<i>R/S</i>)-spirostanol saponin isomer mixtures) were targeted for isolation to verify the speculation. Then, the comprehensive chemical profiling of spirostanol saponins from <i>Y. schidigera</i> was reported here firstly. |
| first_indexed | 2024-03-10T16:53:46Z |
| format | Article |
| id | doaj.art-3e45dc21b81643b3b320a17e9385407c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:53:46Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3e45dc21b81643b3b320a17e9385407c2023-11-20T11:12:50ZengMDPI AGMolecules1420-30492020-08-012517384810.3390/molecules25173848Identification and Structural Analysis of Spirostanol Saponin from <i>Yucca schidigera</i> by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry AnalysisJingya Ruan0Lu Qu1Wei Zhao2Chang Gao3Peijian Huang4Dandan Zheng5Lifeng Han6Haiyang Yu7Zixin Zhang8Yi Zhang9Tao Wang10Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, ChinaTianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, ChinaTianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, ChinaTianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, ChinaTianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, ChinaInstitute of TCM, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, ChinaInstitute of TCM, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, ChinaInstitute of TCM, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, ChinaInstitute of TCM, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, ChinaTianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, ChinaTianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin 301617, China<i>Yucca schidigera</i> Roezl (Mojave), a kind of ornamental plant belonging to the <i>Yucca</i> genus (Agavaceae), whose extract exhibits important roles in food, beverage, cosmetic and feed additives owing to its rich spirostanol saponins. To provide a comprehensive chemical profiling of the spirostanol saponins in it, this study was performed by using a multi-phase liquid chromatography method combining a reversed phase chromatography T<sub>3</sub> column with a normal phase chromatography silica column for the separation and an ESI-Q-Exactive-Orbitrap MS in positive ion mode as the detector. By comparing the retention time and ion fragments with standards, thirty-one spirostanol saponins were identified. In addition, according to the summary of the chromatographic retention behaviors and the MS/MS cleavage patterns and biosynthetic pathway, another seventy-nine spirostanol saponins were speculatively identified, forty ones of which were potentially new ones. Moreover, ten novel spirostanol saponins (three pairs of (25<i>R/S</i>)-spirostanol saponin isomer mixtures) were targeted for isolation to verify the speculation. Then, the comprehensive chemical profiling of spirostanol saponins from <i>Y. schidigera</i> was reported here firstly.https://www.mdpi.com/1420-3049/25/17/3848<i>Yucca schidigera Roezl</i> (Mojave)spirostanol saponinschemical constituents profilingmulti-phase liquid chromatographynormal phase chromatography silica column separationreversed phase liquid chromatography/mass spectrometry analysis |
| spellingShingle | Jingya Ruan Lu Qu Wei Zhao Chang Gao Peijian Huang Dandan Zheng Lifeng Han Haiyang Yu Zixin Zhang Yi Zhang Tao Wang Identification and Structural Analysis of Spirostanol Saponin from <i>Yucca schidigera</i> by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis Molecules <i>Yucca schidigera Roezl</i> (Mojave) spirostanol saponins chemical constituents profiling multi-phase liquid chromatography normal phase chromatography silica column separation reversed phase liquid chromatography/mass spectrometry analysis |
| title | Identification and Structural Analysis of Spirostanol Saponin from <i>Yucca schidigera</i> by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis |
| title_full | Identification and Structural Analysis of Spirostanol Saponin from <i>Yucca schidigera</i> by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis |
| title_fullStr | Identification and Structural Analysis of Spirostanol Saponin from <i>Yucca schidigera</i> by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis |
| title_full_unstemmed | Identification and Structural Analysis of Spirostanol Saponin from <i>Yucca schidigera</i> by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis |
| title_short | Identification and Structural Analysis of Spirostanol Saponin from <i>Yucca schidigera</i> by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis |
| title_sort | identification and structural analysis of spirostanol saponin from i yucca schidigera i by integrating silica gel column chromatography and liquid chromatography mass spectrometry analysis |
| topic | <i>Yucca schidigera Roezl</i> (Mojave) spirostanol saponins chemical constituents profiling multi-phase liquid chromatography normal phase chromatography silica column separation reversed phase liquid chromatography/mass spectrometry analysis |
| url | https://www.mdpi.com/1420-3049/25/17/3848 |
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