Characterization and Genetic Diversity of <i>Pseudomonas syringae</i> pv. <i>syringae</i> Isolates Associated with Rice Bacterial Leaf Spot in Heilongjiang, China
In China, rice is one of the most important cereal crops. Rice bacterial brown leaf spot caused by <i>P. s.</i> pv. <i>syringae</i> is among the most damaging rice diseases in the Heilongjiang Province of China and results in substantial yield losses. In this study, a compreh...
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MDPI AG
2022-05-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/5/720 |
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| author | Lili Peng Songrun Yang Yao Zhang Younis Haseeb Shuang Song Xiaofeng Xu Mingxiu Yang Junhua Zhang |
| author_facet | Lili Peng Songrun Yang Yao Zhang Younis Haseeb Shuang Song Xiaofeng Xu Mingxiu Yang Junhua Zhang |
| author_sort | Lili Peng |
| collection | DOAJ |
| description | In China, rice is one of the most important cereal crops. Rice bacterial brown leaf spot caused by <i>P. s.</i> pv. <i>syringae</i> is among the most damaging rice diseases in the Heilongjiang Province of China and results in substantial yield losses. In this study, a comprehensive analysis of the pathogen, population structure, and genetic diversity within the species was performed. For this purpose, 176 bacterial isolates of <i>P. s.</i> pv. <i>syringae</i> collected from 15 locations were characterized by using biochemical tests such as the LOPAT test, and genetic characterizations such as multilocus sequence analysis (MLSA) and repetitive PCR, using BOX, REP and ERIC primers. Biochemical testing and detection of <i>syrB</i> genes confirm the presence of <i>P. s.</i> pv. <i>syringae,</i> genetic characterization by MLSA and genetic fingerprinting by repetitive PCR confirmed that high genetic heterogeneity exists in the <i>P. s.</i> pv. <i>syringae</i> isolates, and clustering of the tested isolates and reference strains are related with the same genomospecies 1. This work contributes to the physiological classification of the <i>P. s.</i> pv. <i>syringae</i> isolated from Heilongjiang Province, China, and the results present new data concerning the phylogeny and genetic diversity. This type of study about <i>P. s.</i> pv. <i>syringae</i> has been not reported from this region until now. |
| first_indexed | 2024-03-10T03:18:16Z |
| format | Article |
| id | doaj.art-3e47d1ecab5349f5bbfbd222849236de |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T03:18:16Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | Biology |
| spelling | doaj.art-3e47d1ecab5349f5bbfbd222849236de2023-11-23T10:07:30ZengMDPI AGBiology2079-77372022-05-0111572010.3390/biology11050720Characterization and Genetic Diversity of <i>Pseudomonas syringae</i> pv. <i>syringae</i> Isolates Associated with Rice Bacterial Leaf Spot in Heilongjiang, ChinaLili Peng0Songrun Yang1Yao Zhang2Younis Haseeb3Shuang Song4Xiaofeng Xu5Mingxiu Yang6Junhua Zhang7College of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, ChinaIn China, rice is one of the most important cereal crops. Rice bacterial brown leaf spot caused by <i>P. s.</i> pv. <i>syringae</i> is among the most damaging rice diseases in the Heilongjiang Province of China and results in substantial yield losses. In this study, a comprehensive analysis of the pathogen, population structure, and genetic diversity within the species was performed. For this purpose, 176 bacterial isolates of <i>P. s.</i> pv. <i>syringae</i> collected from 15 locations were characterized by using biochemical tests such as the LOPAT test, and genetic characterizations such as multilocus sequence analysis (MLSA) and repetitive PCR, using BOX, REP and ERIC primers. Biochemical testing and detection of <i>syrB</i> genes confirm the presence of <i>P. s.</i> pv. <i>syringae,</i> genetic characterization by MLSA and genetic fingerprinting by repetitive PCR confirmed that high genetic heterogeneity exists in the <i>P. s.</i> pv. <i>syringae</i> isolates, and clustering of the tested isolates and reference strains are related with the same genomospecies 1. This work contributes to the physiological classification of the <i>P. s.</i> pv. <i>syringae</i> isolated from Heilongjiang Province, China, and the results present new data concerning the phylogeny and genetic diversity. This type of study about <i>P. s.</i> pv. <i>syringae</i> has been not reported from this region until now.https://www.mdpi.com/2079-7737/11/5/720rice<i>Pseudomonas syringae</i> pv. <i>syringae</i>bacteriamultilocus sequence analysis (MLSA)repetitive extragenic palindrome (rep) PCR |
| spellingShingle | Lili Peng Songrun Yang Yao Zhang Younis Haseeb Shuang Song Xiaofeng Xu Mingxiu Yang Junhua Zhang Characterization and Genetic Diversity of <i>Pseudomonas syringae</i> pv. <i>syringae</i> Isolates Associated with Rice Bacterial Leaf Spot in Heilongjiang, China Biology rice <i>Pseudomonas syringae</i> pv. <i>syringae</i> bacteria multilocus sequence analysis (MLSA) repetitive extragenic palindrome (rep) PCR |
| title | Characterization and Genetic Diversity of <i>Pseudomonas syringae</i> pv. <i>syringae</i> Isolates Associated with Rice Bacterial Leaf Spot in Heilongjiang, China |
| title_full | Characterization and Genetic Diversity of <i>Pseudomonas syringae</i> pv. <i>syringae</i> Isolates Associated with Rice Bacterial Leaf Spot in Heilongjiang, China |
| title_fullStr | Characterization and Genetic Diversity of <i>Pseudomonas syringae</i> pv. <i>syringae</i> Isolates Associated with Rice Bacterial Leaf Spot in Heilongjiang, China |
| title_full_unstemmed | Characterization and Genetic Diversity of <i>Pseudomonas syringae</i> pv. <i>syringae</i> Isolates Associated with Rice Bacterial Leaf Spot in Heilongjiang, China |
| title_short | Characterization and Genetic Diversity of <i>Pseudomonas syringae</i> pv. <i>syringae</i> Isolates Associated with Rice Bacterial Leaf Spot in Heilongjiang, China |
| title_sort | characterization and genetic diversity of i pseudomonas syringae i pv i syringae i isolates associated with rice bacterial leaf spot in heilongjiang china |
| topic | rice <i>Pseudomonas syringae</i> pv. <i>syringae</i> bacteria multilocus sequence analysis (MLSA) repetitive extragenic palindrome (rep) PCR |
| url | https://www.mdpi.com/2079-7737/11/5/720 |
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