Different Antimicrobial Susceptibility Testing Methods to Determine Vancomycin Susceptibility and MIC for <i>Staphylococcus aureus</i> with Reduced Vancomycin Susceptibility
The methods and results obtained using commercialized automation systems used for antimicrobial susceptibility testing are not entirely consistent. Therefore, we evaluated different antimicrobial susceptibility testing methods to determine vancomycin susceptibility and minimum inhibitory concentrati...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/4/845 |
| _version_ | 1797436123368128512 |
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| author | Hae-Sun Chung Miae Lee |
| author_facet | Hae-Sun Chung Miae Lee |
| author_sort | Hae-Sun Chung |
| collection | DOAJ |
| description | The methods and results obtained using commercialized automation systems used for antimicrobial susceptibility testing are not entirely consistent. Therefore, we evaluated different antimicrobial susceptibility testing methods to determine vancomycin susceptibility and minimum inhibitory concentration (MIC) for <i>Staphylococcus aureus</i> with reduced vancomycin susceptibility (SA-RVS). A total of 128 clinical isolates of <i>S. aureus</i> were tested, including 99 isolates showing an MIC of ≥2 µg/mL using the VITEK2 system (VITEK2). Antimicrobial susceptibility tests were performed using the Sensititre system (Sensititre), Phoenix M50 system (Phoenix), and MicroScan WalkAway 96 Plus system (MicroScan). Vancomycin MICs were determined using the broth microdilution method (BMD) and Etest. Essential agreement and category agreement for each method were compared with BMD results as the reference method. The BMD and Etest showed complete essential agreement (100%). VITEK2, Sensititre, and Phoenix showed high essential agreement (>99%), while MicroScan showed the lowest essential agreement (92.2%). The MIC MICs determined via Etest, VITEK2, and MicroScan tended to be higher than that determined via BMD. When comparing BMD with Etest, the category agreement was 93.8% and minor errors were observed for eight isolates. VITEK2, Sensititre, and Phoenix showed category agreements of 96.1%, 96.1%, and 99.2%, respectively, while MicroScan showed the lowest category agreement of 85.2%. The determination of vancomycin susceptibility and MIC for <i>S. aureus</i> varied among the methods. Caution should be taken when interpreting RVS and intermediate results for <i>S. aureus</i>. For confirmation of SA-RVS results, it would be appropriate to test with BMD or a more reliable testing method. |
| first_indexed | 2024-03-09T10:57:07Z |
| format | Article |
| id | doaj.art-3e4eb0459ae1484dae05b376128eb35f |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T10:57:07Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-3e4eb0459ae1484dae05b376128eb35f2023-12-01T01:31:28ZengMDPI AGDiagnostics2075-44182022-03-0112484510.3390/diagnostics12040845Different Antimicrobial Susceptibility Testing Methods to Determine Vancomycin Susceptibility and MIC for <i>Staphylococcus aureus</i> with Reduced Vancomycin SusceptibilityHae-Sun Chung0Miae Lee1Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul 07804, KoreaDepartment of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul 07804, KoreaThe methods and results obtained using commercialized automation systems used for antimicrobial susceptibility testing are not entirely consistent. Therefore, we evaluated different antimicrobial susceptibility testing methods to determine vancomycin susceptibility and minimum inhibitory concentration (MIC) for <i>Staphylococcus aureus</i> with reduced vancomycin susceptibility (SA-RVS). A total of 128 clinical isolates of <i>S. aureus</i> were tested, including 99 isolates showing an MIC of ≥2 µg/mL using the VITEK2 system (VITEK2). Antimicrobial susceptibility tests were performed using the Sensititre system (Sensititre), Phoenix M50 system (Phoenix), and MicroScan WalkAway 96 Plus system (MicroScan). Vancomycin MICs were determined using the broth microdilution method (BMD) and Etest. Essential agreement and category agreement for each method were compared with BMD results as the reference method. The BMD and Etest showed complete essential agreement (100%). VITEK2, Sensititre, and Phoenix showed high essential agreement (>99%), while MicroScan showed the lowest essential agreement (92.2%). The MIC MICs determined via Etest, VITEK2, and MicroScan tended to be higher than that determined via BMD. When comparing BMD with Etest, the category agreement was 93.8% and minor errors were observed for eight isolates. VITEK2, Sensititre, and Phoenix showed category agreements of 96.1%, 96.1%, and 99.2%, respectively, while MicroScan showed the lowest category agreement of 85.2%. The determination of vancomycin susceptibility and MIC for <i>S. aureus</i> varied among the methods. Caution should be taken when interpreting RVS and intermediate results for <i>S. aureus</i>. For confirmation of SA-RVS results, it would be appropriate to test with BMD or a more reliable testing method.https://www.mdpi.com/2075-4418/12/4/845<i>Staphylococcus aureus</i>vancomycinantimicrobial susceptibility test |
| spellingShingle | Hae-Sun Chung Miae Lee Different Antimicrobial Susceptibility Testing Methods to Determine Vancomycin Susceptibility and MIC for <i>Staphylococcus aureus</i> with Reduced Vancomycin Susceptibility Diagnostics <i>Staphylococcus aureus</i> vancomycin antimicrobial susceptibility test |
| title | Different Antimicrobial Susceptibility Testing Methods to Determine Vancomycin Susceptibility and MIC for <i>Staphylococcus aureus</i> with Reduced Vancomycin Susceptibility |
| title_full | Different Antimicrobial Susceptibility Testing Methods to Determine Vancomycin Susceptibility and MIC for <i>Staphylococcus aureus</i> with Reduced Vancomycin Susceptibility |
| title_fullStr | Different Antimicrobial Susceptibility Testing Methods to Determine Vancomycin Susceptibility and MIC for <i>Staphylococcus aureus</i> with Reduced Vancomycin Susceptibility |
| title_full_unstemmed | Different Antimicrobial Susceptibility Testing Methods to Determine Vancomycin Susceptibility and MIC for <i>Staphylococcus aureus</i> with Reduced Vancomycin Susceptibility |
| title_short | Different Antimicrobial Susceptibility Testing Methods to Determine Vancomycin Susceptibility and MIC for <i>Staphylococcus aureus</i> with Reduced Vancomycin Susceptibility |
| title_sort | different antimicrobial susceptibility testing methods to determine vancomycin susceptibility and mic for i staphylococcus aureus i with reduced vancomycin susceptibility |
| topic | <i>Staphylococcus aureus</i> vancomycin antimicrobial susceptibility test |
| url | https://www.mdpi.com/2075-4418/12/4/845 |
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