Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic study
A simple and rapid liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay method has been developed and fully validated for simultaneous quantification of pioglitazone and candesartan in human plasma. Irbesartan was used as an internal standard. The analytes were extracted from human plasm...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-06-01
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| Series: | Journal of Pharmaceutical Analysis |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177912000123 |
| _version_ | 1818448099909042176 |
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| author | Vijaya Kumari Karra Nageswara Rao Pilli Jaswanth Kumar Inamadugu J.V.L.N. Seshagiri Rao |
| author_facet | Vijaya Kumari Karra Nageswara Rao Pilli Jaswanth Kumar Inamadugu J.V.L.N. Seshagiri Rao |
| author_sort | Vijaya Kumari Karra |
| collection | DOAJ |
| description | A simple and rapid liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay method has been developed and fully validated for simultaneous quantification of pioglitazone and candesartan in human plasma. Irbesartan was used as an internal standard. The analytes were extracted from human plasma samples by solid-phase extraction technique using a Strata-X 33 μm polymeric sorbent. The reconstituted samples were chromatographed on a C18 column by using a 80:20 (v/v) mixture of acetonitrile and 0.1% formic acid as the mobile phase at a flow rate of 0.8 mL/min. The calibration curves obtained were linear (râ¥0.99) over the concentration range of 15â3000 ng/mL for pioglitazone and 5â608 ng/mL for candesartan. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits. A run time of 2.7 min for each sample made it possible to analyze more than 300 plasma samples per day. The proposed method was found to be applicable to clinical studies. Keywords: Pioglitazone, Candesartan, Human plasma, Solid-phase extraction, LC-MS/MS, Pharmacokinetics |
| first_indexed | 2024-12-14T20:14:08Z |
| format | Article |
| id | doaj.art-3e4f12d3ac094f2ea7a48d9486f608f6 |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-12-14T20:14:08Z |
| publishDate | 2012-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-3e4f12d3ac094f2ea7a48d9486f608f62022-12-21T22:48:53ZengElsevierJournal of Pharmaceutical Analysis2095-17792012-06-0123167173Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic studyVijaya Kumari Karra0Nageswara Rao Pilli1Jaswanth Kumar Inamadugu2J.V.L.N. Seshagiri Rao3University College of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad-500085, IndiaUniversity College of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad-500085, IndiaAnalytical and Environmental Chemistry Division, Department of Chemistry, Sri Venkateswara University, Tirupati-517502, IndiaCollege of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, India; Corresponding author. +91 9491766577.A simple and rapid liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay method has been developed and fully validated for simultaneous quantification of pioglitazone and candesartan in human plasma. Irbesartan was used as an internal standard. The analytes were extracted from human plasma samples by solid-phase extraction technique using a Strata-X 33 μm polymeric sorbent. The reconstituted samples were chromatographed on a C18 column by using a 80:20 (v/v) mixture of acetonitrile and 0.1% formic acid as the mobile phase at a flow rate of 0.8 mL/min. The calibration curves obtained were linear (râ¥0.99) over the concentration range of 15â3000 ng/mL for pioglitazone and 5â608 ng/mL for candesartan. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits. A run time of 2.7 min for each sample made it possible to analyze more than 300 plasma samples per day. The proposed method was found to be applicable to clinical studies. Keywords: Pioglitazone, Candesartan, Human plasma, Solid-phase extraction, LC-MS/MS, Pharmacokineticshttp://www.sciencedirect.com/science/article/pii/S2095177912000123 |
| spellingShingle | Vijaya Kumari Karra Nageswara Rao Pilli Jaswanth Kumar Inamadugu J.V.L.N. Seshagiri Rao Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic study Journal of Pharmaceutical Analysis |
| title | Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic study |
| title_full | Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic study |
| title_fullStr | Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic study |
| title_full_unstemmed | Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic study |
| title_short | Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic study |
| title_sort | simultaneous determination of pioglitazone and candesartan in human plasma by lc ms ms and its application to a human pharmacokinetic study |
| url | http://www.sciencedirect.com/science/article/pii/S2095177912000123 |
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