Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxum
Dendrobium chrysotoxum is considered as an important ornamental dendrobium because of its strong and long-lasting floral scent. Nevertheless, few information is known about the dynamic changes and related formation mechanism of dendrobium floral scent at different flowering stages. In this study, th...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1030492/full |
| _version_ | 1811186871253860352 |
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| author | Zhihui Du Yuxuan Jin Weize Wang Kuaifei Xia Zhilin Chen |
| author_facet | Zhihui Du Yuxuan Jin Weize Wang Kuaifei Xia Zhilin Chen |
| author_sort | Zhihui Du |
| collection | DOAJ |
| description | Dendrobium chrysotoxum is considered as an important ornamental dendrobium because of its strong and long-lasting floral scent. Nevertheless, few information is known about the dynamic changes and related formation mechanism of dendrobium floral scent at different flowering stages. In this study, the characteristics and biosynthetic mechanism of floral scent in D. chrysotoxum during flowering was revealed by using widely-targeted volatilomics (WTV) combined with transcriptome analysis. Over 500 kinds of volatile organic compounds (VOCs) were detected in the floral scents of D. chrysotoxum, which improved the knowledge about floral scent components of dendrobium. A total of 153 differential VOCs and 4,487 differentially expressed genes (DEGs) were identified between flowers of different flowering stages, respectively. The results for both volatilomics and transcriptomics data indicated that terpenes and related genes played an important role in the formation of floral characteristics of D. chrysotoxum. But in general, the expression of genes showed an opposite trend to the accumulation of metabolites during flowering, suggesting that the regulation of floral scent biosynthesis might have started at the budding stage in D. chrysotoxum. Additionally, a transcriptional metabolic regulatory network consisting of terpenes, terpene synthases and candidate transcription factors was established. This research is the first systematic and comprehensive exploration of floral characteristics and related mechanisms during flowering in D. chrysotoxum. It provides basis for exploration of mechanisms on the floral scents and the breeding of aromatic dendrobium. |
| first_indexed | 2024-04-11T13:52:38Z |
| format | Article |
| id | doaj.art-3e4f3fd567794dafb10baaf88b40f25f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T13:52:38Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3e4f3fd567794dafb10baaf88b40f25f2022-12-22T04:20:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10304921030492Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxumZhihui Du0Yuxuan Jin1Weize Wang2Kuaifei Xia3Zhilin Chen4Guizhou Horticulture Institute, Guizhou Academy of Agricultural Sciences, Guiyang, ChinaGuizhou Horticulture Institute, Guizhou Academy of Agricultural Sciences, Guiyang, ChinaGuizhou Horticulture Institute, Guizhou Academy of Agricultural Sciences, Guiyang, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, ChinaGuizhou Horticulture Institute, Guizhou Academy of Agricultural Sciences, Guiyang, ChinaDendrobium chrysotoxum is considered as an important ornamental dendrobium because of its strong and long-lasting floral scent. Nevertheless, few information is known about the dynamic changes and related formation mechanism of dendrobium floral scent at different flowering stages. In this study, the characteristics and biosynthetic mechanism of floral scent in D. chrysotoxum during flowering was revealed by using widely-targeted volatilomics (WTV) combined with transcriptome analysis. Over 500 kinds of volatile organic compounds (VOCs) were detected in the floral scents of D. chrysotoxum, which improved the knowledge about floral scent components of dendrobium. A total of 153 differential VOCs and 4,487 differentially expressed genes (DEGs) were identified between flowers of different flowering stages, respectively. The results for both volatilomics and transcriptomics data indicated that terpenes and related genes played an important role in the formation of floral characteristics of D. chrysotoxum. But in general, the expression of genes showed an opposite trend to the accumulation of metabolites during flowering, suggesting that the regulation of floral scent biosynthesis might have started at the budding stage in D. chrysotoxum. Additionally, a transcriptional metabolic regulatory network consisting of terpenes, terpene synthases and candidate transcription factors was established. This research is the first systematic and comprehensive exploration of floral characteristics and related mechanisms during flowering in D. chrysotoxum. It provides basis for exploration of mechanisms on the floral scents and the breeding of aromatic dendrobium.https://www.frontiersin.org/articles/10.3389/fpls.2022.1030492/fullDendrobium chrysotoxumfloral scentwidely-targeted volatilomicsterpenesRNA-Seq |
| spellingShingle | Zhihui Du Yuxuan Jin Weize Wang Kuaifei Xia Zhilin Chen Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxum Frontiers in Plant Science Dendrobium chrysotoxum floral scent widely-targeted volatilomics terpenes RNA-Seq |
| title | Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxum |
| title_full | Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxum |
| title_fullStr | Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxum |
| title_full_unstemmed | Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxum |
| title_short | Molecular and metabolic insights into floral scent biosynthesis during flowering in Dendrobium chrysotoxum |
| title_sort | molecular and metabolic insights into floral scent biosynthesis during flowering in dendrobium chrysotoxum |
| topic | Dendrobium chrysotoxum floral scent widely-targeted volatilomics terpenes RNA-Seq |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1030492/full |
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