Artificial Propagation and Embryonic Growth of Stinging Catfish, Heteropneustes fossilis (Bloch, 1794) Using S-GnRHa (Salmon Gonadotropin Releasing Hormone Analogue)
An experiment was performed to examine the potentiality of a synthetic hormone an-alogue in the artificial propagation i.e., the embryonic and larval development of sting-ing catfish (Heteropneustes fossilis). Broodfish were injected with S-GnRHa at the concentrations of 1, 2.5, and 5 ml.k...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2021-06-01
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| Series: | Journal of Tropical Life Science |
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| Online Access: | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1597/pdf |
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| author | Tasrick Araf Md. Alal Hossen Gourab Chowdhury Mohammad Amzad Hossain Md. Arifur Rahman Mohammed Mahbub Iqba |
| author_facet | Tasrick Araf Md. Alal Hossen Gourab Chowdhury Mohammad Amzad Hossain Md. Arifur Rahman Mohammed Mahbub Iqba |
| author_sort | Tasrick Araf |
| collection | DOAJ |
| description | An experiment was performed to examine the potentiality of a synthetic hormone an-alogue in the artificial propagation i.e., the embryonic and larval development of sting-ing catfish (Heteropneustes fossilis). Broodfish were injected with S-GnRHa at the concentrations of 1, 2.5, and 5 ml.kg-1of body weight (BW) in females and at 0.5, 1.125 and 2.5 ml.kg-1 BW in malestreatment groups T1, T2and T3, respectively, with three replicates in each group. The fishes were induced to spawn, and ovulation oc-curred at about 10-11 hours after the hormone injection. Results showed highest fer-tilization (83.11±1.36) and hatching rates (89.56±1.04) in T2, whereas ovulation rate was 100% in all treatment groups. First cleavage was observed at 30 minutes of post-fertilization. Embryonic developmental period sequentially for 2-cell, 4-cell, 8-cell, 16-cell, 32-cell, 64-cell, Morula (Improve), Blastula, Gastrula, somatic formation, yolk-plug, twisting movement and pre-hatching occurred at00:30, 00:45, 01:10, 01:30, 02:00, 02:30, 03:00, 04:00, 06:40, 09:00-18:00, 19:00, 20:00-21:00 and 22:00 hours, respectively. Hatching occurred after 23:00 hours of fertilization. Current re-sults suggest the feasibility of S-GnRHa as an effective synthetic hormone in the arti-ficial propagation of H. fossilis. |
| first_indexed | 2024-03-09T06:46:10Z |
| format | Article |
| id | doaj.art-3e5073dcad3f46378f8799787edbe1e4 |
| institution | Directory Open Access Journal |
| issn | 2087-5517 |
| language | English |
| last_indexed | 2024-03-09T06:46:10Z |
| publishDate | 2021-06-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-3e5073dcad3f46378f8799787edbe1e42023-12-03T10:35:15ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172021-06-01111141149http://dx.doi.org/10.11594/jtls.11.02.03Artificial Propagation and Embryonic Growth of Stinging Catfish, Heteropneustes fossilis (Bloch, 1794) Using S-GnRHa (Salmon Gonadotropin Releasing Hormone Analogue)Tasrick Araf0Md. Alal Hossen1Gourab Chowdhury2Mohammad Amzad Hossain3Md. Arifur Rahman4Mohammed Mahbub Iqba5Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, BangladeshDepartment of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, BangladeshDepartment of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, BangladeshDepartment of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, BangladeshDepartment of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Patuakhali-8602, BangladeshDepartment of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet-3100, BangladeshAn experiment was performed to examine the potentiality of a synthetic hormone an-alogue in the artificial propagation i.e., the embryonic and larval development of sting-ing catfish (Heteropneustes fossilis). Broodfish were injected with S-GnRHa at the concentrations of 1, 2.5, and 5 ml.kg-1of body weight (BW) in females and at 0.5, 1.125 and 2.5 ml.kg-1 BW in malestreatment groups T1, T2and T3, respectively, with three replicates in each group. The fishes were induced to spawn, and ovulation oc-curred at about 10-11 hours after the hormone injection. Results showed highest fer-tilization (83.11±1.36) and hatching rates (89.56±1.04) in T2, whereas ovulation rate was 100% in all treatment groups. First cleavage was observed at 30 minutes of post-fertilization. Embryonic developmental period sequentially for 2-cell, 4-cell, 8-cell, 16-cell, 32-cell, 64-cell, Morula (Improve), Blastula, Gastrula, somatic formation, yolk-plug, twisting movement and pre-hatching occurred at00:30, 00:45, 01:10, 01:30, 02:00, 02:30, 03:00, 04:00, 06:40, 09:00-18:00, 19:00, 20:00-21:00 and 22:00 hours, respectively. Hatching occurred after 23:00 hours of fertilization. Current re-sults suggest the feasibility of S-GnRHa as an effective synthetic hormone in the arti-ficial propagation of H. fossilis.https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1597/pdfartificial propagationcleavageembryonic growths-gnrhasomatic formation |
| spellingShingle | Tasrick Araf Md. Alal Hossen Gourab Chowdhury Mohammad Amzad Hossain Md. Arifur Rahman Mohammed Mahbub Iqba Artificial Propagation and Embryonic Growth of Stinging Catfish, Heteropneustes fossilis (Bloch, 1794) Using S-GnRHa (Salmon Gonadotropin Releasing Hormone Analogue) Journal of Tropical Life Science artificial propagation cleavage embryonic growth s-gnrha somatic formation |
| title | Artificial Propagation and Embryonic Growth of Stinging Catfish, Heteropneustes fossilis (Bloch, 1794) Using S-GnRHa (Salmon Gonadotropin Releasing Hormone Analogue) |
| title_full | Artificial Propagation and Embryonic Growth of Stinging Catfish, Heteropneustes fossilis (Bloch, 1794) Using S-GnRHa (Salmon Gonadotropin Releasing Hormone Analogue) |
| title_fullStr | Artificial Propagation and Embryonic Growth of Stinging Catfish, Heteropneustes fossilis (Bloch, 1794) Using S-GnRHa (Salmon Gonadotropin Releasing Hormone Analogue) |
| title_full_unstemmed | Artificial Propagation and Embryonic Growth of Stinging Catfish, Heteropneustes fossilis (Bloch, 1794) Using S-GnRHa (Salmon Gonadotropin Releasing Hormone Analogue) |
| title_short | Artificial Propagation and Embryonic Growth of Stinging Catfish, Heteropneustes fossilis (Bloch, 1794) Using S-GnRHa (Salmon Gonadotropin Releasing Hormone Analogue) |
| title_sort | artificial propagation and embryonic growth of stinging catfish heteropneustes fossilis bloch 1794 using s gnrha salmon gonadotropin releasing hormone analogue |
| topic | artificial propagation cleavage embryonic growth s-gnrha somatic formation |
| url | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/1597/pdf |
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