High-Resolution Ultrasound Imaging for Non-Invasive Characterization of Acute Wound Healing in Radiation Injury on Guinea Pig Skin Tissue
Purpose: High-resolution ultrasound imaging is a non-invasive and objective appraisal. Ultrasound imaging accomplishes the target assessment and follow-up of radiation-induced skin injury. The study aimed to investigate the complete anatomical and structural alternations of acute wound healing i...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-12-01
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| Series: | Frontiers in Biomedical Technologies |
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| Online Access: | https://fbt.tums.ac.ir/index.php/fbt/article/view/642 |
| _version_ | 1797359888222912512 |
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| author | Zeinab Hormozi-Moghaddam Manijhe Mokhtari-Dizaji Mohammad Ali Nilforoshzade Mohsen Bakhshande Sona Zare |
| author_facet | Zeinab Hormozi-Moghaddam Manijhe Mokhtari-Dizaji Mohammad Ali Nilforoshzade Mohsen Bakhshande Sona Zare |
| author_sort | Zeinab Hormozi-Moghaddam |
| collection | DOAJ |
| description |
Purpose: High-resolution ultrasound imaging is a non-invasive and objective appraisal. Ultrasound imaging accomplishes the target assessment and follow-up of radiation-induced skin injury.
The study aimed to investigate the complete anatomical and structural alternations of acute wound healing in skin tissue radiation injury after cell therapy with high-frequency ultrasound imaging techniques.
Materials and Methods: Female guinea pigs (250 g) were divided into 3 groups: (a) controls, consisting of non-treated guinea pigs; (b) radiation-treated; (c) radiation-treated receiving adipose-derived mesenchymal stem cells. Acute radiation-induced skin injury was induced by a single fraction of X-ray irradiation of 60Gy to a 3.0×3.0-cm area with a 1.3-cm bolus on 100-cm SSD in the abdominal skin tissue. Ultrasonic imaging of the depth and quality of healing in the skin tissue was performed by processing ultrasound images at 40-MHz and 75-MHz frequencies.
Results: Skin thickness indicated a significant difference between the treatment and control groups on Day 10 after 60 Gy irradiation (P<0.05). The highest skin thickness was observed in the irradiated group, and the lowest skin thickness was found in the stem cell treatment group.
Conclusion: Evaluation of skin thickness, wound depth, and scar formation is important for the proper assessment and management of wound healing in stem cell therapy of radiation-induced skin damage. High-resolution ultrasound at 40- and 75-MHz frequencies is a major non-invasive method providing unprecedented insight into determining the characterization of the skin, particularly in the context of wound healing.
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| first_indexed | 2024-03-08T15:30:17Z |
| format | Article |
| id | doaj.art-3e521ddc49ac41769884ca92339cf88f |
| institution | Directory Open Access Journal |
| issn | 2345-5837 |
| language | English |
| last_indexed | 2024-03-08T15:30:17Z |
| publishDate | 2023-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Frontiers in Biomedical Technologies |
| spelling | doaj.art-3e521ddc49ac41769884ca92339cf88f2024-01-10T05:59:30ZengTehran University of Medical SciencesFrontiers in Biomedical Technologies2345-58372023-12-0111110.18502/fbt.v11i1.14517High-Resolution Ultrasound Imaging for Non-Invasive Characterization of Acute Wound Healing in Radiation Injury on Guinea Pig Skin TissueZeinab Hormozi-Moghaddam0Manijhe Mokhtari-Dizaji1Mohammad Ali Nilforoshzade2Mohsen Bakhshande3Sona Zare4Radiation Biology Research Center, Iran University of Medical Sciences (IUMS), Tehran, IranDepartment of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranSkin and Stem Cells Research Center, Tehran University of Medical Sciences, Tehran, IranDepartment of Radiology Technology, Allied Medical Faculty, Shahid Beheshti University of Medical Sciences, Tehran, IranSkin and Stem Cells Research Center, Tehran University of Medical Sciences, Tehran, Iran Purpose: High-resolution ultrasound imaging is a non-invasive and objective appraisal. Ultrasound imaging accomplishes the target assessment and follow-up of radiation-induced skin injury. The study aimed to investigate the complete anatomical and structural alternations of acute wound healing in skin tissue radiation injury after cell therapy with high-frequency ultrasound imaging techniques. Materials and Methods: Female guinea pigs (250 g) were divided into 3 groups: (a) controls, consisting of non-treated guinea pigs; (b) radiation-treated; (c) radiation-treated receiving adipose-derived mesenchymal stem cells. Acute radiation-induced skin injury was induced by a single fraction of X-ray irradiation of 60Gy to a 3.0×3.0-cm area with a 1.3-cm bolus on 100-cm SSD in the abdominal skin tissue. Ultrasonic imaging of the depth and quality of healing in the skin tissue was performed by processing ultrasound images at 40-MHz and 75-MHz frequencies. Results: Skin thickness indicated a significant difference between the treatment and control groups on Day 10 after 60 Gy irradiation (P<0.05). The highest skin thickness was observed in the irradiated group, and the lowest skin thickness was found in the stem cell treatment group. Conclusion: Evaluation of skin thickness, wound depth, and scar formation is important for the proper assessment and management of wound healing in stem cell therapy of radiation-induced skin damage. High-resolution ultrasound at 40- and 75-MHz frequencies is a major non-invasive method providing unprecedented insight into determining the characterization of the skin, particularly in the context of wound healing. https://fbt.tums.ac.ir/index.php/fbt/article/view/642High-Resolution Ultrasound ImagingRadiationSkin; Stem Cell TherapyWound Healing |
| spellingShingle | Zeinab Hormozi-Moghaddam Manijhe Mokhtari-Dizaji Mohammad Ali Nilforoshzade Mohsen Bakhshande Sona Zare High-Resolution Ultrasound Imaging for Non-Invasive Characterization of Acute Wound Healing in Radiation Injury on Guinea Pig Skin Tissue Frontiers in Biomedical Technologies High-Resolution Ultrasound Imaging Radiation Skin ; Stem Cell Therapy Wound Healing |
| title | High-Resolution Ultrasound Imaging for Non-Invasive Characterization of Acute Wound Healing in Radiation Injury on Guinea Pig Skin Tissue |
| title_full | High-Resolution Ultrasound Imaging for Non-Invasive Characterization of Acute Wound Healing in Radiation Injury on Guinea Pig Skin Tissue |
| title_fullStr | High-Resolution Ultrasound Imaging for Non-Invasive Characterization of Acute Wound Healing in Radiation Injury on Guinea Pig Skin Tissue |
| title_full_unstemmed | High-Resolution Ultrasound Imaging for Non-Invasive Characterization of Acute Wound Healing in Radiation Injury on Guinea Pig Skin Tissue |
| title_short | High-Resolution Ultrasound Imaging for Non-Invasive Characterization of Acute Wound Healing in Radiation Injury on Guinea Pig Skin Tissue |
| title_sort | high resolution ultrasound imaging for non invasive characterization of acute wound healing in radiation injury on guinea pig skin tissue |
| topic | High-Resolution Ultrasound Imaging Radiation Skin ; Stem Cell Therapy Wound Healing |
| url | https://fbt.tums.ac.ir/index.php/fbt/article/view/642 |
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