Tissue distribution and changes in dopamine during development and stress responses in Malus germplasm
Dopamine is a catecholamine and an anti-oxidant which functions in responses to stress and it interacts with plant hormones to mediate plant development. At present, there are few studies on the functions of dopamine in apple. This study developed a method for dopamine determination which was used t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311920635900 |
| _version_ | 1818083592143634432 |
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| author | Zhi-jun ZHANG Jing ZHANG Zhong-wen TANG Yan-peng WANG Teng-teng GAO Xiao-min LIU Feng-wang MA Chao LI |
| author_facet | Zhi-jun ZHANG Jing ZHANG Zhong-wen TANG Yan-peng WANG Teng-teng GAO Xiao-min LIU Feng-wang MA Chao LI |
| author_sort | Zhi-jun ZHANG |
| collection | DOAJ |
| description | Dopamine is a catecholamine and an anti-oxidant which functions in responses to stress and it interacts with plant hormones to mediate plant development. At present, there are few studies on the functions of dopamine in apple. This study developed a method for dopamine determination which was used to analyze dopamine in Malus germplasm, in order to clarify the tissue distribution, developmental changes, diurnal variations, and stress responses in apple trees. First, the proposed method was verified. The linear range of quantification was robust from 0.1 to 20 ng mL−1. The instrumental, inter-day precision, and sample repeatability relative standard deviations were 1.024, 5.607, and 7.237%, respectively. The spiked recovery was greater than 100%, indicating the feasibility of the method and its suitability for the rapid analysis of dopamine in Malus. Next, the dopamine content was measured in 322 Malus tissues. The results showed that the dopamine level in Malus was low and the average dopamine content in leaf was higher than in peel and flesh. The dopamine had a skewed distribution that deviated to the right in cultivars and wild accessions. Finally, the tissue specificity, developmental changes, diurnal changes, and responses to stress were analyzed. In cultivar ‘Pinova’ (Malus domestica), the dopamine concentration was the highest in leaf buds and lowest in flesh. The dopamine contents in leaf and flesh decreased with the growth and development of cultivar ‘Liangxiang’ (Malus domestica). The dopamine content of apple leaves was higher after either drought or salinity stress as compared to the control. In this study, a dopamine detection method for apple was established based on HPLC-MS and shown to be a robust approach. This study provides a framework for future research on elucidating the tissue distribution, developmental changes, diurnal variation, and stress responses of dopamine in apple trees. |
| first_indexed | 2024-12-10T19:40:26Z |
| format | Article |
| id | doaj.art-3e538db30f0b47deaeb1e8c6d5e844fe |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-10T19:40:26Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-3e538db30f0b47deaeb1e8c6d5e844fe2022-12-22T01:36:01ZengElsevierJournal of Integrative Agriculture2095-31192022-03-01213710724Tissue distribution and changes in dopamine during development and stress responses in Malus germplasmZhi-jun ZHANG0Jing ZHANG1Zhong-wen TANG2Yan-peng WANG3Teng-teng GAO4Xiao-min LIU5Feng-wang MA6Chao LI7State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaCorrespondence LI Chao, Tel/Fax: +86-29-87082648; State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R.ChinaDopamine is a catecholamine and an anti-oxidant which functions in responses to stress and it interacts with plant hormones to mediate plant development. At present, there are few studies on the functions of dopamine in apple. This study developed a method for dopamine determination which was used to analyze dopamine in Malus germplasm, in order to clarify the tissue distribution, developmental changes, diurnal variations, and stress responses in apple trees. First, the proposed method was verified. The linear range of quantification was robust from 0.1 to 20 ng mL−1. The instrumental, inter-day precision, and sample repeatability relative standard deviations were 1.024, 5.607, and 7.237%, respectively. The spiked recovery was greater than 100%, indicating the feasibility of the method and its suitability for the rapid analysis of dopamine in Malus. Next, the dopamine content was measured in 322 Malus tissues. The results showed that the dopamine level in Malus was low and the average dopamine content in leaf was higher than in peel and flesh. The dopamine had a skewed distribution that deviated to the right in cultivars and wild accessions. Finally, the tissue specificity, developmental changes, diurnal changes, and responses to stress were analyzed. In cultivar ‘Pinova’ (Malus domestica), the dopamine concentration was the highest in leaf buds and lowest in flesh. The dopamine contents in leaf and flesh decreased with the growth and development of cultivar ‘Liangxiang’ (Malus domestica). The dopamine content of apple leaves was higher after either drought or salinity stress as compared to the control. In this study, a dopamine detection method for apple was established based on HPLC-MS and shown to be a robust approach. This study provides a framework for future research on elucidating the tissue distribution, developmental changes, diurnal variation, and stress responses of dopamine in apple trees.http://www.sciencedirect.com/science/article/pii/S2095311920635900dopamineMalusHPLC-MScultivarwild accession |
| spellingShingle | Zhi-jun ZHANG Jing ZHANG Zhong-wen TANG Yan-peng WANG Teng-teng GAO Xiao-min LIU Feng-wang MA Chao LI Tissue distribution and changes in dopamine during development and stress responses in Malus germplasm Journal of Integrative Agriculture dopamine Malus HPLC-MS cultivar wild accession |
| title | Tissue distribution and changes in dopamine during development and stress responses in Malus germplasm |
| title_full | Tissue distribution and changes in dopamine during development and stress responses in Malus germplasm |
| title_fullStr | Tissue distribution and changes in dopamine during development and stress responses in Malus germplasm |
| title_full_unstemmed | Tissue distribution and changes in dopamine during development and stress responses in Malus germplasm |
| title_short | Tissue distribution and changes in dopamine during development and stress responses in Malus germplasm |
| title_sort | tissue distribution and changes in dopamine during development and stress responses in malus germplasm |
| topic | dopamine Malus HPLC-MS cultivar wild accession |
| url | http://www.sciencedirect.com/science/article/pii/S2095311920635900 |
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