Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran

and are among the most important Gram-negative bacteria that can cause nosocomial infections, especially in burn patients. It is important to determine genetic relationships in different clinical specimens as well as between clinical and environmental specimens, which can aid in detecting the sourc...

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Main Authors: Azimi, Leila, Alaghehbandan, Reza, Asadian, Mahla, Alinejad, Faranak, Lari, Abdolaziz Rastegar
Format: Article
Language:deu
Published: German Medical Science GMS Publishing House 2019-01-01
Series:GMS Hygiene and Infection Control
Subjects:
Online Access:http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000317.shtml
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author Azimi, Leila
Alaghehbandan, Reza
Asadian, Mahla
Alinejad, Faranak
Lari, Abdolaziz Rastegar
author_facet Azimi, Leila
Alaghehbandan, Reza
Asadian, Mahla
Alinejad, Faranak
Lari, Abdolaziz Rastegar
author_sort Azimi, Leila
collection DOAJ
description and are among the most important Gram-negative bacteria that can cause nosocomial infections, especially in burn patients. It is important to determine genetic relationships in different clinical specimens as well as between clinical and environmental specimens, which can aid in detecting the source of infection. The aim of this study was to investigate multi-drug resistant and spread in a burn hospital, Tehran, Iran. After identification, antibiotic susceptibility testing of all isolates was conducted according to the CLSI guidelines. Further, pulsed-field gel electrophoresis (PFGE) was performed for molecular typing. 97 clinical and 33 environmental specimens were collected. 40 (55%) clinical strains of and were highly drug resistant. PFGE findings showed similar genetic features to those seen in multi-drug resistant and/or extensively drug resistant and in clinical and environmental isolates. Inhibition of bacterial spread in the hospital can help to control health care-associated infection and subsequently decrease the morbidity and mortality in hospitalized patients, particularly immunocompromised populations such as burn patients.
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spelling doaj.art-3e53aa9966f94265ac362b27479be4252022-12-21T19:01:39ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262019-01-0114Doc0110.3205/dgkh000317Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, IranAzimi, Leila0Alaghehbandan, Reza1Asadian, Mahla2Alinejad, Faranak3Lari, Abdolaziz Rastegar4Pediatric Infectious Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Pathology, University of British Columbia, Royal Columbian Hospital, Vancouver, BC, CanadaDivision of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranBurn Research Center, Iran University of Medical Sciences, Tehran, IranDepartment of Microbiology, Iran University of Medical Sciences, Tehran, Iranand are among the most important Gram-negative bacteria that can cause nosocomial infections, especially in burn patients. It is important to determine genetic relationships in different clinical specimens as well as between clinical and environmental specimens, which can aid in detecting the source of infection. The aim of this study was to investigate multi-drug resistant and spread in a burn hospital, Tehran, Iran. After identification, antibiotic susceptibility testing of all isolates was conducted according to the CLSI guidelines. Further, pulsed-field gel electrophoresis (PFGE) was performed for molecular typing. 97 clinical and 33 environmental specimens were collected. 40 (55%) clinical strains of and were highly drug resistant. PFGE findings showed similar genetic features to those seen in multi-drug resistant and/or extensively drug resistant and in clinical and environmental isolates. Inhibition of bacterial spread in the hospital can help to control health care-associated infection and subsequently decrease the morbidity and mortality in hospitalized patients, particularly immunocompromised populations such as burn patients.http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000317.shtmlhealth care-associated infectionP. aeruginosaK. pneumoniaeantibiotic resistancegenetic relationshippulsed-field gel electrophoresis
spellingShingle Azimi, Leila
Alaghehbandan, Reza
Asadian, Mahla
Alinejad, Faranak
Lari, Abdolaziz Rastegar
Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran
GMS Hygiene and Infection Control
health care-associated infection
P. aeruginosa
K. pneumoniae
antibiotic resistance
genetic relationship
pulsed-field gel electrophoresis
title Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran
title_full Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran
title_fullStr Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran
title_full_unstemmed Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran
title_short Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran
title_sort multi drug resistant pseudomonas aeruginosa and klebsiella pneumoniae circulation in a burn hospital tehran iran
topic health care-associated infection
P. aeruginosa
K. pneumoniae
antibiotic resistance
genetic relationship
pulsed-field gel electrophoresis
url http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000317.shtml
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AT alaghehbandanreza multidrugresistantpseudomonasaeruginosaandklebsiellapneumoniaecirculationinaburnhospitaltehraniran
AT asadianmahla multidrugresistantpseudomonasaeruginosaandklebsiellapneumoniaecirculationinaburnhospitaltehraniran
AT alinejadfaranak multidrugresistantpseudomonasaeruginosaandklebsiellapneumoniaecirculationinaburnhospitaltehraniran
AT lariabdolazizrastegar multidrugresistantpseudomonasaeruginosaandklebsiellapneumoniaecirculationinaburnhospitaltehraniran