Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran
and are among the most important Gram-negative bacteria that can cause nosocomial infections, especially in burn patients. It is important to determine genetic relationships in different clinical specimens as well as between clinical and environmental specimens, which can aid in detecting the sourc...
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Format: | Article |
Language: | deu |
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German Medical Science GMS Publishing House
2019-01-01
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Series: | GMS Hygiene and Infection Control |
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Online Access: | http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000317.shtml |
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author | Azimi, Leila Alaghehbandan, Reza Asadian, Mahla Alinejad, Faranak Lari, Abdolaziz Rastegar |
author_facet | Azimi, Leila Alaghehbandan, Reza Asadian, Mahla Alinejad, Faranak Lari, Abdolaziz Rastegar |
author_sort | Azimi, Leila |
collection | DOAJ |
description | and are among the most important Gram-negative bacteria that can cause nosocomial infections, especially in burn patients. It is important to determine genetic relationships in different clinical specimens as well as between clinical and environmental specimens, which can aid in detecting the source of infection. The aim of this study was to investigate multi-drug resistant and spread in a burn hospital, Tehran, Iran. After identification, antibiotic susceptibility testing of all isolates was conducted according to the CLSI guidelines. Further, pulsed-field gel electrophoresis (PFGE) was performed for molecular typing. 97 clinical and 33 environmental specimens were collected. 40 (55%) clinical strains of and were highly drug resistant. PFGE findings showed similar genetic features to those seen in multi-drug resistant and/or extensively drug resistant and in clinical and environmental isolates. Inhibition of bacterial spread in the hospital can help to control health care-associated infection and subsequently decrease the morbidity and mortality in hospitalized patients, particularly immunocompromised populations such as burn patients. |
first_indexed | 2024-12-21T13:52:24Z |
format | Article |
id | doaj.art-3e53aa9966f94265ac362b27479be425 |
institution | Directory Open Access Journal |
issn | 2196-5226 |
language | deu |
last_indexed | 2024-12-21T13:52:24Z |
publishDate | 2019-01-01 |
publisher | German Medical Science GMS Publishing House |
record_format | Article |
series | GMS Hygiene and Infection Control |
spelling | doaj.art-3e53aa9966f94265ac362b27479be4252022-12-21T19:01:39ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262019-01-0114Doc0110.3205/dgkh000317Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, IranAzimi, Leila0Alaghehbandan, Reza1Asadian, Mahla2Alinejad, Faranak3Lari, Abdolaziz Rastegar4Pediatric Infectious Research Center, Research Institute for Children's Health, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Pathology, University of British Columbia, Royal Columbian Hospital, Vancouver, BC, CanadaDivision of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranBurn Research Center, Iran University of Medical Sciences, Tehran, IranDepartment of Microbiology, Iran University of Medical Sciences, Tehran, Iranand are among the most important Gram-negative bacteria that can cause nosocomial infections, especially in burn patients. It is important to determine genetic relationships in different clinical specimens as well as between clinical and environmental specimens, which can aid in detecting the source of infection. The aim of this study was to investigate multi-drug resistant and spread in a burn hospital, Tehran, Iran. After identification, antibiotic susceptibility testing of all isolates was conducted according to the CLSI guidelines. Further, pulsed-field gel electrophoresis (PFGE) was performed for molecular typing. 97 clinical and 33 environmental specimens were collected. 40 (55%) clinical strains of and were highly drug resistant. PFGE findings showed similar genetic features to those seen in multi-drug resistant and/or extensively drug resistant and in clinical and environmental isolates. Inhibition of bacterial spread in the hospital can help to control health care-associated infection and subsequently decrease the morbidity and mortality in hospitalized patients, particularly immunocompromised populations such as burn patients.http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000317.shtmlhealth care-associated infectionP. aeruginosaK. pneumoniaeantibiotic resistancegenetic relationshippulsed-field gel electrophoresis |
spellingShingle | Azimi, Leila Alaghehbandan, Reza Asadian, Mahla Alinejad, Faranak Lari, Abdolaziz Rastegar Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran GMS Hygiene and Infection Control health care-associated infection P. aeruginosa K. pneumoniae antibiotic resistance genetic relationship pulsed-field gel electrophoresis |
title | Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran |
title_full | Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran |
title_fullStr | Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran |
title_full_unstemmed | Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran |
title_short | Multi-drug resistant Pseudomonas aeruginosa and Klebsiella pneumoniae circulation in a burn hospital, Tehran, Iran |
title_sort | multi drug resistant pseudomonas aeruginosa and klebsiella pneumoniae circulation in a burn hospital tehran iran |
topic | health care-associated infection P. aeruginosa K. pneumoniae antibiotic resistance genetic relationship pulsed-field gel electrophoresis |
url | http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000317.shtml |
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