Genome-wide identification and analysis of Catharanthus roseus RLK1-like kinases in Nicotiana benthamiana
Abstract Background The Catharanthus roseus RLK1-like kinase (CrRLK1L) is a subfamily of the RLK gene family, and members are sensors of cell wall integrity and regulators of cell polarity growth. Recent studies have also shown that members of this subfamily are involved in plant immunity. Nicotiana...
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-03208-x |
| _version_ | 1818718436272898048 |
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| author | Shaofei Rao Xinyang Wu Hongying Zheng Yuwen Lu Jiejun Peng Guanwei Wu Jianping Chen Fei Yan |
| author_facet | Shaofei Rao Xinyang Wu Hongying Zheng Yuwen Lu Jiejun Peng Guanwei Wu Jianping Chen Fei Yan |
| author_sort | Shaofei Rao |
| collection | DOAJ |
| description | Abstract Background The Catharanthus roseus RLK1-like kinase (CrRLK1L) is a subfamily of the RLK gene family, and members are sensors of cell wall integrity and regulators of cell polarity growth. Recent studies have also shown that members of this subfamily are involved in plant immunity. Nicotiana benthamiana is a model plant widely used in the study of plant-pathogen interactions. However, the members of the NbCrRLK1L subfamily and their response to pathogens have not been reported. Results In this study, a total of 31 CrRLK1L members were identified in the N. benthamiana genome, and these can be divided into 6 phylogenetic groups (I-VI). The members in each group have similar exon-intron structures and conserved motifs. NbCrRLK1Ls were predicted to be regulated by cis-acting elements such as STRE, TCA, ABRE, etc., and to be the target of transcription factors such as Dof and MYB. The expression profiles of the 16 selected NbCrRLK1Ls were determined by quantitative PCR. Most NbCrRLK1Ls were highly expressed in leaves but there were different and diverse expression patterns in other tissues. Inoculation with the bacterium Pseudomonas syringae or with Turnip mosaic virus significantly altered the transcript levels of the tested genes, suggesting that NbCrRLK1Ls may be involved in the response to pathogens. Conclusions This study systematically identified the CrRLK1L members in N. benthamiana, and analyzed their tissue-specific expression and gene expression profiles in response to different pathogens and two pathogens associated molecular patterns (PAMPs). This research lays the foundation for exploring the function of NbCrRLK1Ls in plant-microbe interactions. |
| first_indexed | 2024-12-17T19:51:01Z |
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| id | doaj.art-3e545e1fb6884367beab8f80997147f2 |
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| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-17T19:51:01Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj.art-3e545e1fb6884367beab8f80997147f22022-12-21T21:34:43ZengBMCBMC Plant Biology1471-22292021-09-0121111210.1186/s12870-021-03208-xGenome-wide identification and analysis of Catharanthus roseus RLK1-like kinases in Nicotiana benthamianaShaofei Rao0Xinyang Wu1Hongying Zheng2Yuwen Lu3Jiejun Peng4Guanwei Wu5Jianping Chen6Fei Yan7State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo UniversityState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo UniversityAbstract Background The Catharanthus roseus RLK1-like kinase (CrRLK1L) is a subfamily of the RLK gene family, and members are sensors of cell wall integrity and regulators of cell polarity growth. Recent studies have also shown that members of this subfamily are involved in plant immunity. Nicotiana benthamiana is a model plant widely used in the study of plant-pathogen interactions. However, the members of the NbCrRLK1L subfamily and their response to pathogens have not been reported. Results In this study, a total of 31 CrRLK1L members were identified in the N. benthamiana genome, and these can be divided into 6 phylogenetic groups (I-VI). The members in each group have similar exon-intron structures and conserved motifs. NbCrRLK1Ls were predicted to be regulated by cis-acting elements such as STRE, TCA, ABRE, etc., and to be the target of transcription factors such as Dof and MYB. The expression profiles of the 16 selected NbCrRLK1Ls were determined by quantitative PCR. Most NbCrRLK1Ls were highly expressed in leaves but there were different and diverse expression patterns in other tissues. Inoculation with the bacterium Pseudomonas syringae or with Turnip mosaic virus significantly altered the transcript levels of the tested genes, suggesting that NbCrRLK1Ls may be involved in the response to pathogens. Conclusions This study systematically identified the CrRLK1L members in N. benthamiana, and analyzed their tissue-specific expression and gene expression profiles in response to different pathogens and two pathogens associated molecular patterns (PAMPs). This research lays the foundation for exploring the function of NbCrRLK1Ls in plant-microbe interactions.https://doi.org/10.1186/s12870-021-03208-xCatharanthus roseus RLK1-like kinaseNicotiana benthamianaGenome-wideExpression analysis |
| spellingShingle | Shaofei Rao Xinyang Wu Hongying Zheng Yuwen Lu Jiejun Peng Guanwei Wu Jianping Chen Fei Yan Genome-wide identification and analysis of Catharanthus roseus RLK1-like kinases in Nicotiana benthamiana BMC Plant Biology Catharanthus roseus RLK1-like kinase Nicotiana benthamiana Genome-wide Expression analysis |
| title | Genome-wide identification and analysis of Catharanthus roseus RLK1-like kinases in Nicotiana benthamiana |
| title_full | Genome-wide identification and analysis of Catharanthus roseus RLK1-like kinases in Nicotiana benthamiana |
| title_fullStr | Genome-wide identification and analysis of Catharanthus roseus RLK1-like kinases in Nicotiana benthamiana |
| title_full_unstemmed | Genome-wide identification and analysis of Catharanthus roseus RLK1-like kinases in Nicotiana benthamiana |
| title_short | Genome-wide identification and analysis of Catharanthus roseus RLK1-like kinases in Nicotiana benthamiana |
| title_sort | genome wide identification and analysis of catharanthus roseus rlk1 like kinases in nicotiana benthamiana |
| topic | Catharanthus roseus RLK1-like kinase Nicotiana benthamiana Genome-wide Expression analysis |
| url | https://doi.org/10.1186/s12870-021-03208-x |
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