Down-Regulation of <i>SlGRAS10</i> in Tomato Confers Abiotic Stress Tolerance
Adverse environmental factors like salt stress, drought, and extreme temperatures, cause damage to plant growth, development, and crop yield. GRAS transcription factors (TFs) have numerous functions in biological processes. Some studies have reported that the GRAS protein family plays significant fu...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/12/5/623 |
| _version_ | 1797536773507645440 |
|---|---|
| author | Sidra Habib Yee Yee Lwin Ning Li |
| author_facet | Sidra Habib Yee Yee Lwin Ning Li |
| author_sort | Sidra Habib |
| collection | DOAJ |
| description | Adverse environmental factors like salt stress, drought, and extreme temperatures, cause damage to plant growth, development, and crop yield. GRAS transcription factors (TFs) have numerous functions in biological processes. Some studies have reported that the GRAS protein family plays significant functions in plant growth and development under abiotic stresses. In this study, we demonstrated the functional characterization of a tomato <i>SlGRAS10</i> gene under abiotic stresses such as salt stress and drought. Down-regulation of <i>SlGRAS10</i> by RNA interference (RNAi) produced dwarf plants with smaller leaves, internode lengths, and enhanced flavonoid accumulation. We studied the effects of abiotic stresses on RNAi and wild-type (WT) plants. Moreover, <i>SlGRAS10</i>-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants. Down-regulation of <i>SlGRAS10</i> significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) to reduce the effects of reactive oxygen species (ROS) such as O<sup>2−</sup> and H<sub>2</sub>O<sub>2</sub>. Malondialdehyde (MDA) and proline contents were remarkably high in <i>SlGRAS10</i>-RNAi plants. Furthermore, the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions. Collectively, our conclusions emphasized the significant function of <i>SlGRAS10</i> as a stress tolerate transcription factor in a certain variety of abiotic stress tolerance by enhancing osmotic potential, flavonoid biosynthesis, and ROS scavenging system in the tomato plant. |
| first_indexed | 2024-03-10T12:04:36Z |
| format | Article |
| id | doaj.art-3e55fe59773d4ed39d7b1769e6f8ff13 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T12:04:36Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-3e55fe59773d4ed39d7b1769e6f8ff132023-11-21T16:38:43ZengMDPI AGGenes2073-44252021-04-0112562310.3390/genes12050623Down-Regulation of <i>SlGRAS10</i> in Tomato Confers Abiotic Stress ToleranceSidra Habib0Yee Yee Lwin1Ning Li2Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing 400044, ChinaCollege of Life Sciences, Henan Normal University, Xinxiang 453007, ChinaAdverse environmental factors like salt stress, drought, and extreme temperatures, cause damage to plant growth, development, and crop yield. GRAS transcription factors (TFs) have numerous functions in biological processes. Some studies have reported that the GRAS protein family plays significant functions in plant growth and development under abiotic stresses. In this study, we demonstrated the functional characterization of a tomato <i>SlGRAS10</i> gene under abiotic stresses such as salt stress and drought. Down-regulation of <i>SlGRAS10</i> by RNA interference (RNAi) produced dwarf plants with smaller leaves, internode lengths, and enhanced flavonoid accumulation. We studied the effects of abiotic stresses on RNAi and wild-type (WT) plants. Moreover, <i>SlGRAS10</i>-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants. Down-regulation of <i>SlGRAS10</i> significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) to reduce the effects of reactive oxygen species (ROS) such as O<sup>2−</sup> and H<sub>2</sub>O<sub>2</sub>. Malondialdehyde (MDA) and proline contents were remarkably high in <i>SlGRAS10</i>-RNAi plants. Furthermore, the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions. Collectively, our conclusions emphasized the significant function of <i>SlGRAS10</i> as a stress tolerate transcription factor in a certain variety of abiotic stress tolerance by enhancing osmotic potential, flavonoid biosynthesis, and ROS scavenging system in the tomato plant.https://www.mdpi.com/2073-4425/12/5/623<i>SlGRAS10</i>abiotic stresstolerancetomatoROS scavenging systemflavonoid |
| spellingShingle | Sidra Habib Yee Yee Lwin Ning Li Down-Regulation of <i>SlGRAS10</i> in Tomato Confers Abiotic Stress Tolerance Genes <i>SlGRAS10</i> abiotic stress tolerance tomato ROS scavenging system flavonoid |
| title | Down-Regulation of <i>SlGRAS10</i> in Tomato Confers Abiotic Stress Tolerance |
| title_full | Down-Regulation of <i>SlGRAS10</i> in Tomato Confers Abiotic Stress Tolerance |
| title_fullStr | Down-Regulation of <i>SlGRAS10</i> in Tomato Confers Abiotic Stress Tolerance |
| title_full_unstemmed | Down-Regulation of <i>SlGRAS10</i> in Tomato Confers Abiotic Stress Tolerance |
| title_short | Down-Regulation of <i>SlGRAS10</i> in Tomato Confers Abiotic Stress Tolerance |
| title_sort | down regulation of i slgras10 i in tomato confers abiotic stress tolerance |
| topic | <i>SlGRAS10</i> abiotic stress tolerance tomato ROS scavenging system flavonoid |
| url | https://www.mdpi.com/2073-4425/12/5/623 |
| work_keys_str_mv | AT sidrahabib downregulationofislgras10iintomatoconfersabioticstresstolerance AT yeeyeelwin downregulationofislgras10iintomatoconfersabioticstresstolerance AT ningli downregulationofislgras10iintomatoconfersabioticstresstolerance |