Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model
The administration of cardiosphere-derived cells (CDCs) after acute myocardial infarction (AMI) is very promising. CDC encapsulation in alginate-poly-<span style="font-variant: small-caps;">l</span>-lysine-alginate (APA) could increase cell survival and adherence. The intraperi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/11/1824 |
| _version_ | 1797508782528397312 |
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| author | Claudia Báez-Díaz Virginia Blanco-Blázquez Francisco Miguel Sánchez-Margallo Esther López Helena Martín Albert Espona-Noguera Javier G. Casado Jesús Ciriza José Luis Pedraz Verónica Crisóstomo |
| author_facet | Claudia Báez-Díaz Virginia Blanco-Blázquez Francisco Miguel Sánchez-Margallo Esther López Helena Martín Albert Espona-Noguera Javier G. Casado Jesús Ciriza José Luis Pedraz Verónica Crisóstomo |
| author_sort | Claudia Báez-Díaz |
| collection | DOAJ |
| description | The administration of cardiosphere-derived cells (CDCs) after acute myocardial infarction (AMI) is very promising. CDC encapsulation in alginate-poly-<span style="font-variant: small-caps;">l</span>-lysine-alginate (APA) could increase cell survival and adherence. The intrapericardial (IP) approach potentially achieves high concentrations of the therapeutic agent in the infarcted area. We aimed to evaluate IP therapy using a saline vehicle as a control (CON), a dose of 30 × 10<sup>6</sup> CDCs (CDCs) or APA microcapsules containing 30 × 10<sup>6</sup> CDCs (APA-CDCs) at 72 h in a porcine AMI model. Magnetic resonance imaging (MRI) was used to determine the left ventricular ejection fraction (LVEF), infarct size (IS), and indexed end diastolic and systolic volumes (EDVi; ESVi) pre- and 10 weeks post-injection. Programmed electrical stimulation (PES) was performed to test arrhythmia inducibility before euthanasia. Histopathological analysis was carried out afterwards. The IP infusion was successful in all animals. At 10 weeks, MRI revealed significantly higher LVEF in the APA-CDC group compared with CON. No significant differences were observed among groups in IS, EDVi, ESVi, PES and histopathological analyses. In conclusion, the IP injection of CDCs (microencapsulated or not) was feasible and safe 72 h post-AMI in the porcine model. Moreover, CDCs APA encapsulation could have a beneficial effect on cardiac function, reflected by a higher LVEF at 10 weeks. |
| first_indexed | 2024-03-10T05:08:47Z |
| format | Article |
| id | doaj.art-3e561752daf44aaa95fd92ae106f53c2 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T05:08:47Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-3e561752daf44aaa95fd92ae106f53c22023-11-23T00:58:07ZengMDPI AGPharmaceutics1999-49232021-11-011311182410.3390/pharmaceutics13111824Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction ModelClaudia Báez-Díaz0Virginia Blanco-Blázquez1Francisco Miguel Sánchez-Margallo2Esther López3Helena Martín4Albert Espona-Noguera5Javier G. Casado6Jesús Ciriza7José Luis Pedraz8Verónica Crisóstomo9CIBERCV, Instituto de Salud Carlos III, 28029 Madrid, SpainCIBERCV, Instituto de Salud Carlos III, 28029 Madrid, SpainCIBERCV, Instituto de Salud Carlos III, 28029 Madrid, SpainFundación Centro de Cirugía de Mínima Invasión Jesús Usón, 10071 Cáceres, SpainFundación Centro de Cirugía de Mínima Invasión Jesús Usón, 10071 Cáceres, SpainCentro de Investigaciones y Estudios Avanzados Lucio Lascaray (CIEA), Laboratorio de Desarrollo y Evaluación de Medicamentos, 01006 Vitoria Gasteiz, SpainImmunology Unit-Institute of Molecular Pathology Biomarkers, Veterinary Faculty, University of Extremadura, 10003 Cáceres, SpainCIBER bbn, Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigaciones y Estudios Avanzados Lucio Lascaray (CIEA), Laboratorio de Desarrollo y Evaluación de Medicamentos, 01006 Vitoria Gasteiz, SpainCIBERCV, Instituto de Salud Carlos III, 28029 Madrid, SpainThe administration of cardiosphere-derived cells (CDCs) after acute myocardial infarction (AMI) is very promising. CDC encapsulation in alginate-poly-<span style="font-variant: small-caps;">l</span>-lysine-alginate (APA) could increase cell survival and adherence. The intrapericardial (IP) approach potentially achieves high concentrations of the therapeutic agent in the infarcted area. We aimed to evaluate IP therapy using a saline vehicle as a control (CON), a dose of 30 × 10<sup>6</sup> CDCs (CDCs) or APA microcapsules containing 30 × 10<sup>6</sup> CDCs (APA-CDCs) at 72 h in a porcine AMI model. Magnetic resonance imaging (MRI) was used to determine the left ventricular ejection fraction (LVEF), infarct size (IS), and indexed end diastolic and systolic volumes (EDVi; ESVi) pre- and 10 weeks post-injection. Programmed electrical stimulation (PES) was performed to test arrhythmia inducibility before euthanasia. Histopathological analysis was carried out afterwards. The IP infusion was successful in all animals. At 10 weeks, MRI revealed significantly higher LVEF in the APA-CDC group compared with CON. No significant differences were observed among groups in IS, EDVi, ESVi, PES and histopathological analyses. In conclusion, the IP injection of CDCs (microencapsulated or not) was feasible and safe 72 h post-AMI in the porcine model. Moreover, CDCs APA encapsulation could have a beneficial effect on cardiac function, reflected by a higher LVEF at 10 weeks.https://www.mdpi.com/1999-4923/13/11/1824CDCsAMIintrapericardialmicrocapsulesAPAswine |
| spellingShingle | Claudia Báez-Díaz Virginia Blanco-Blázquez Francisco Miguel Sánchez-Margallo Esther López Helena Martín Albert Espona-Noguera Javier G. Casado Jesús Ciriza José Luis Pedraz Verónica Crisóstomo Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model Pharmaceutics CDCs AMI intrapericardial microcapsules APA swine |
| title | Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model |
| title_full | Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model |
| title_fullStr | Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model |
| title_full_unstemmed | Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model |
| title_short | Intrapericardial Delivery of APA-Microcapsules as Promising Stem Cell Therapy Carriers in an Experimental Acute Myocardial Infarction Model |
| title_sort | intrapericardial delivery of apa microcapsules as promising stem cell therapy carriers in an experimental acute myocardial infarction model |
| topic | CDCs AMI intrapericardial microcapsules APA swine |
| url | https://www.mdpi.com/1999-4923/13/11/1824 |
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