Engineering <i>Yarrowia lipolytica</i> for the Synthesis of Glutathione from Organic By-Products
Tripeptide glutathione, which plays important roles in many cellular mechanisms, is also a biotechnology-oriented molecule with applications in medicine, food and cosmetic. Here, the engineering of the yeast <i>Yarrowia lipolytica</i> for the production of this metabolite at high titer v...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/4/611 |
| _version_ | 1797569766930513920 |
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| author | Diem T. H. Do Patrick Fickers |
| author_facet | Diem T. H. Do Patrick Fickers |
| author_sort | Diem T. H. Do |
| collection | DOAJ |
| description | Tripeptide glutathione, which plays important roles in many cellular mechanisms, is also a biotechnology-oriented molecule with applications in medicine, food and cosmetic. Here, the engineering of the yeast <i>Yarrowia lipolytica</i> for the production of this metabolite at high titer values from various agro-industrial by-products is reported. The constitutive overexpression of the glutathione biosynthetic genes <i>GSH1</i> and <i>GSH2</i> encoding respectively γ-glutamylcysteine synthetase and glutathione synthetase, together with the <i>INU1</i> gene from <i>Kluyveromyces marxianus</i> encoding inulinase yielded a glutathione titer value and a productivity of 644 nmol/mg protein and 510 µmol/g<sub>DCW</sub>, respectively. These values were obtained during bioreactor batch cultures in a medium exclusively comprising an extract of Jerusalem artichoke tuber, used as a source of inulin, and ammonium sulfate, used as a nitrogen source. |
| first_indexed | 2024-03-10T20:16:13Z |
| format | Article |
| id | doaj.art-3e582f66545042418492d3c490f48acd |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T20:16:13Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3e582f66545042418492d3c490f48acd2023-11-19T22:31:41ZengMDPI AGMicroorganisms2076-26072020-04-018461110.3390/microorganisms8040611Engineering <i>Yarrowia lipolytica</i> for the Synthesis of Glutathione from Organic By-ProductsDiem T. H. Do0Patrick Fickers1Microbial Processes and Interactions, TERRA Teaching and Research Center, University of Liège - Gembloux Agro-Bio Tech, Av. de la Faculté, 2B, 5030 Gembloux, BelgiumMicrobial Processes and Interactions, TERRA Teaching and Research Center, University of Liège - Gembloux Agro-Bio Tech, Av. de la Faculté, 2B, 5030 Gembloux, BelgiumTripeptide glutathione, which plays important roles in many cellular mechanisms, is also a biotechnology-oriented molecule with applications in medicine, food and cosmetic. Here, the engineering of the yeast <i>Yarrowia lipolytica</i> for the production of this metabolite at high titer values from various agro-industrial by-products is reported. The constitutive overexpression of the glutathione biosynthetic genes <i>GSH1</i> and <i>GSH2</i> encoding respectively γ-glutamylcysteine synthetase and glutathione synthetase, together with the <i>INU1</i> gene from <i>Kluyveromyces marxianus</i> encoding inulinase yielded a glutathione titer value and a productivity of 644 nmol/mg protein and 510 µmol/g<sub>DCW</sub>, respectively. These values were obtained during bioreactor batch cultures in a medium exclusively comprising an extract of Jerusalem artichoke tuber, used as a source of inulin, and ammonium sulfate, used as a nitrogen source.https://www.mdpi.com/2076-2607/8/4/611glutathioneinulin<i>Yarrowia lipolytica</i><i>GSH1</i><i>GSH</i>2<i>INU1</i> |
| spellingShingle | Diem T. H. Do Patrick Fickers Engineering <i>Yarrowia lipolytica</i> for the Synthesis of Glutathione from Organic By-Products Microorganisms glutathione inulin <i>Yarrowia lipolytica</i> <i>GSH1</i> <i>GSH</i>2 <i>INU1</i> |
| title | Engineering <i>Yarrowia lipolytica</i> for the Synthesis of Glutathione from Organic By-Products |
| title_full | Engineering <i>Yarrowia lipolytica</i> for the Synthesis of Glutathione from Organic By-Products |
| title_fullStr | Engineering <i>Yarrowia lipolytica</i> for the Synthesis of Glutathione from Organic By-Products |
| title_full_unstemmed | Engineering <i>Yarrowia lipolytica</i> for the Synthesis of Glutathione from Organic By-Products |
| title_short | Engineering <i>Yarrowia lipolytica</i> for the Synthesis of Glutathione from Organic By-Products |
| title_sort | engineering i yarrowia lipolytica i for the synthesis of glutathione from organic by products |
| topic | glutathione inulin <i>Yarrowia lipolytica</i> <i>GSH1</i> <i>GSH</i>2 <i>INU1</i> |
| url | https://www.mdpi.com/2076-2607/8/4/611 |
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