RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish Nutrition
Nowadays, aquaculture provides more than 50% of fish consumed worldwide but faces new issues that challenge its sustainability. One of them relies on the replacement of fish meal (FM) in aquaculture feeds by other protein sources without deeply affecting the whole organism’s homeostasis. Multiple st...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/9/8/1754 |
| _version_ | 1827712579815866368 |
|---|---|
| author | Guillaume Morin Karine Pinel Karine Dias Iban Seiliez Florian Beaumatin |
| author_facet | Guillaume Morin Karine Pinel Karine Dias Iban Seiliez Florian Beaumatin |
| author_sort | Guillaume Morin |
| collection | DOAJ |
| description | Nowadays, aquaculture provides more than 50% of fish consumed worldwide but faces new issues that challenge its sustainability. One of them relies on the replacement of fish meal (FM) in aquaculture feeds by other protein sources without deeply affecting the whole organism’s homeostasis. Multiple strategies have already been tested using in vivo approaches, but they hardly managed to cope with the multifactorial problems related to the complexities of fish biology together with new feed formulations. In this context, rainbow trout (RT) is particularly concerned by these problems, since, as a carnivorous fish, dietary proteins provide the amino acids required to supply most of its energetic metabolism. Surprisingly, we noticed that in vitro approaches considering RT cell lines as models to study RT amino acid metabolism were never previously used. Therefore, we decided to investigate if, and how, three major pathways described, in other species, to be regulated by amino acid and to control cellular homeostasis were functional in a RT cell line called RTH-149—namely, the mechanistic Target Of Rapamycin (mTOR), autophagy and the general control nonderepressible 2 (GCN2) pathways. Our results not only demonstrated that these three pathways were functional in RTH-149 cells, but they also highlighted some RT specificities with respect to the time response, amino acid dependencies and the activation levels of their downstream targets. Altogether, this article demonstrated, for the first time, that RT cell lines could represent an interesting alternative of in vivo experimentations for the study of fish nutrition-related questions. |
| first_indexed | 2024-03-10T18:17:45Z |
| format | Article |
| id | doaj.art-3e6354518047458d8cd4355b3fcb130c |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T18:17:45Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3e6354518047458d8cd4355b3fcb130c2023-11-20T07:33:51ZengMDPI AGCells2073-44092020-07-0198175410.3390/cells9081754RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish NutritionGuillaume Morin0Karine Pinel1Karine Dias2Iban Seiliez3Florian Beaumatin4Université de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, INRAE, NUMEA, 64310 Saint-Pée-sur-Nivelle, FranceNowadays, aquaculture provides more than 50% of fish consumed worldwide but faces new issues that challenge its sustainability. One of them relies on the replacement of fish meal (FM) in aquaculture feeds by other protein sources without deeply affecting the whole organism’s homeostasis. Multiple strategies have already been tested using in vivo approaches, but they hardly managed to cope with the multifactorial problems related to the complexities of fish biology together with new feed formulations. In this context, rainbow trout (RT) is particularly concerned by these problems, since, as a carnivorous fish, dietary proteins provide the amino acids required to supply most of its energetic metabolism. Surprisingly, we noticed that in vitro approaches considering RT cell lines as models to study RT amino acid metabolism were never previously used. Therefore, we decided to investigate if, and how, three major pathways described, in other species, to be regulated by amino acid and to control cellular homeostasis were functional in a RT cell line called RTH-149—namely, the mechanistic Target Of Rapamycin (mTOR), autophagy and the general control nonderepressible 2 (GCN2) pathways. Our results not only demonstrated that these three pathways were functional in RTH-149 cells, but they also highlighted some RT specificities with respect to the time response, amino acid dependencies and the activation levels of their downstream targets. Altogether, this article demonstrated, for the first time, that RT cell lines could represent an interesting alternative of in vivo experimentations for the study of fish nutrition-related questions.https://www.mdpi.com/2073-4409/9/8/1754aquaculturerainbow troutRTH-149 cell linenutritionamino acidsautophagy |
| spellingShingle | Guillaume Morin Karine Pinel Karine Dias Iban Seiliez Florian Beaumatin RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish Nutrition Cells aquaculture rainbow trout RTH-149 cell line nutrition amino acids autophagy |
| title | RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish Nutrition |
| title_full | RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish Nutrition |
| title_fullStr | RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish Nutrition |
| title_full_unstemmed | RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish Nutrition |
| title_short | RTH-149 Cell Line, a Useful Tool to Decipher Molecular Mechanisms Related to Fish Nutrition |
| title_sort | rth 149 cell line a useful tool to decipher molecular mechanisms related to fish nutrition |
| topic | aquaculture rainbow trout RTH-149 cell line nutrition amino acids autophagy |
| url | https://www.mdpi.com/2073-4409/9/8/1754 |
| work_keys_str_mv | AT guillaumemorin rth149celllineausefultooltodeciphermolecularmechanismsrelatedtofishnutrition AT karinepinel rth149celllineausefultooltodeciphermolecularmechanismsrelatedtofishnutrition AT karinedias rth149celllineausefultooltodeciphermolecularmechanismsrelatedtofishnutrition AT ibanseiliez rth149celllineausefultooltodeciphermolecularmechanismsrelatedtofishnutrition AT florianbeaumatin rth149celllineausefultooltodeciphermolecularmechanismsrelatedtofishnutrition |