LncRNA CASC9 promotes cell proliferation and invasion in osteosarcoma through targeting miR-874-3p/SOX12 axis
Abstract Background Osteosarcoma (OS) is a common primary malignant bone tumor. This study aimed to explore the biological role of long on-coding RNA (lncRNA) CASC9 and its regulatory mechanism in OC. Methods The CASC9 expressions in OS cells and tissues were measured using qRT-PCR. The functional r...
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-022-03340-w |
| _version_ | 1797985956962238464 |
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| author | Haiyan Qiu Di Yang Xiaolin Li Fabo Feng |
| author_facet | Haiyan Qiu Di Yang Xiaolin Li Fabo Feng |
| author_sort | Haiyan Qiu |
| collection | DOAJ |
| description | Abstract Background Osteosarcoma (OS) is a common primary malignant bone tumor. This study aimed to explore the biological role of long on-coding RNA (lncRNA) CASC9 and its regulatory mechanism in OC. Methods The CASC9 expressions in OS cells and tissues were measured using qRT-PCR. The functional role of CASC9 in OC was studied using MTT assay, colony formation assay, transwell invasion assay, and xenograft tumor assay. In addition, the mechanism of CASC9 function was determined using luciferase reporter assay. Western blot was used to analyze protein expressions in our paper. Results LncRNA CASC9 was found to be up-regulated in OS. Knockdown of CASC9 inhibited the proliferation and invasion of OS cells. Besides, miR-874-3p was identified as the target of CASC9, and SOX12 acted as a potential target of miR-874-3p. The down-regulation of miR-874-3p recovered the reduction in cell invasion and proliferation in vitro which were induced by CASC9 knockdown and delayed the tumor progression in vivo. Conclusion LncRNA CASC9 promotes cell proliferation and invasion in OS via miR-874-3p/SOX12 axis. Our study might provide novel biomarkers and potential therapeutic targets for OS treatment. |
| first_indexed | 2024-04-11T07:26:16Z |
| format | Article |
| id | doaj.art-3e68214dccbe49629f9565d6b8d266d2 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T07:26:16Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-3e68214dccbe49629f9565d6b8d266d22022-12-22T04:37:04ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2022-10-0117111110.1186/s13018-022-03340-wLncRNA CASC9 promotes cell proliferation and invasion in osteosarcoma through targeting miR-874-3p/SOX12 axisHaiyan Qiu0Di Yang1Xiaolin Li2Fabo Feng3Department of Endocrinology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of MedicineCenter for Plastic and Reconstructive Surgery, Department of Orthopedics, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Center for Plastic and Reconstructive Surgery, Department of Orthopedics, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Center for Plastic and Reconstructive Surgery, Department of Orthopedics, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College)Abstract Background Osteosarcoma (OS) is a common primary malignant bone tumor. This study aimed to explore the biological role of long on-coding RNA (lncRNA) CASC9 and its regulatory mechanism in OC. Methods The CASC9 expressions in OS cells and tissues were measured using qRT-PCR. The functional role of CASC9 in OC was studied using MTT assay, colony formation assay, transwell invasion assay, and xenograft tumor assay. In addition, the mechanism of CASC9 function was determined using luciferase reporter assay. Western blot was used to analyze protein expressions in our paper. Results LncRNA CASC9 was found to be up-regulated in OS. Knockdown of CASC9 inhibited the proliferation and invasion of OS cells. Besides, miR-874-3p was identified as the target of CASC9, and SOX12 acted as a potential target of miR-874-3p. The down-regulation of miR-874-3p recovered the reduction in cell invasion and proliferation in vitro which were induced by CASC9 knockdown and delayed the tumor progression in vivo. Conclusion LncRNA CASC9 promotes cell proliferation and invasion in OS via miR-874-3p/SOX12 axis. Our study might provide novel biomarkers and potential therapeutic targets for OS treatment.https://doi.org/10.1186/s13018-022-03340-wOsteosarcomalncRNACASC9miR-874-3pSOX12 |
| spellingShingle | Haiyan Qiu Di Yang Xiaolin Li Fabo Feng LncRNA CASC9 promotes cell proliferation and invasion in osteosarcoma through targeting miR-874-3p/SOX12 axis Journal of Orthopaedic Surgery and Research Osteosarcoma lncRNA CASC9 miR-874-3p SOX12 |
| title | LncRNA CASC9 promotes cell proliferation and invasion in osteosarcoma through targeting miR-874-3p/SOX12 axis |
| title_full | LncRNA CASC9 promotes cell proliferation and invasion in osteosarcoma through targeting miR-874-3p/SOX12 axis |
| title_fullStr | LncRNA CASC9 promotes cell proliferation and invasion in osteosarcoma through targeting miR-874-3p/SOX12 axis |
| title_full_unstemmed | LncRNA CASC9 promotes cell proliferation and invasion in osteosarcoma through targeting miR-874-3p/SOX12 axis |
| title_short | LncRNA CASC9 promotes cell proliferation and invasion in osteosarcoma through targeting miR-874-3p/SOX12 axis |
| title_sort | lncrna casc9 promotes cell proliferation and invasion in osteosarcoma through targeting mir 874 3p sox12 axis |
| topic | Osteosarcoma lncRNA CASC9 miR-874-3p SOX12 |
| url | https://doi.org/10.1186/s13018-022-03340-w |
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