In vitro study of the anticancer activity of various doxorubicin-containing dispersions
Introduction: The aim of this research was to study the impact of various doxorubicin (Dox)-containing nanofluids, e.g. singlewalled carbon nanotube (SWCNT)+Dox, graphene oxide (GO)+Dox and DextranPNIPAM (copolymer)+Dox mixtures on HeLa cells (human transformed cervix epithelial cells, as a model fo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2019-01-01
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| Series: | BioImpacts |
| Subjects: | |
| Online Access: | https://bi.tbzmed.ac.ir/PDF/bi-9-57.pdf |
| _version_ | 1818449676181962752 |
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| author | Tatiana Matvienko Viktoriya Sokolova Svitlana Prylutska Yuliia Harahuts Nataliya Kutsevol Viktor Kostjukov Maxim Evstigneev Yuriy Prylutskyy Matthias Epple Uwe Ritter |
| author_facet | Tatiana Matvienko Viktoriya Sokolova Svitlana Prylutska Yuliia Harahuts Nataliya Kutsevol Viktor Kostjukov Maxim Evstigneev Yuriy Prylutskyy Matthias Epple Uwe Ritter |
| author_sort | Tatiana Matvienko |
| collection | DOAJ |
| description | Introduction: The aim of this research was to study the impact of various doxorubicin (Dox)-containing nanofluids, e.g. singlewalled carbon nanotube (SWCNT)+Dox, graphene oxide (GO)+Dox and DextranPNIPAM (copolymer)+Dox mixtures on HeLa cells (human transformed cervix epithelial cells, as a model for cancer cells) depending on their concentration. Methods: Structural analysis of GO+Dox complex was accomplished using Hartree-Fock level of theory in 6-31G** basis set in Gaussian. Dynamic light scattering (DLS), zeta-potential, scanning electron microscopy and confocal laser scanning microscopy were used. The cell viability was analyzed by the MTT assay. Results: The viability of HeLa cells was studied with the MTT assay after the incubation with various Dox-containing dispersions depending on their concentration. The size of the particles was determined by DLS. The morphology of the nanoparticles (NPs) was studied by scanning electron microscopy and their uptake into cells was visualized by confocal laser scanning microscopy. It was found that the Dextran-PNIPAM+Dox nanofluid in contrast to Dox alone showed higher toxicity towards HeLa cells up to 80% after 24 hours of incubation, whereas the SWCNT+Dox and GO+Dox nanofluids at the same concentrations protected cells from Dox. Conclusion: The importance of Dextran-PNIPAM copolymer as a universal platform for drug delivery was established, and the huge potential of Dextran-PNIPAM+Dox NPs as novel anticancer agents was noted. Based on the in vitro study of the SWCNT+Dox and GO+Dox nanofluids, it was concluded that SWCNT and GO NPs can be effective cytoprotectors against the highly toxic drugs. |
| first_indexed | 2024-12-14T20:39:11Z |
| format | Article |
| id | doaj.art-3e6907600340413bb824c4301d79604a |
| institution | Directory Open Access Journal |
| issn | 2228-5660 2228-5652 |
| language | English |
| last_indexed | 2024-12-14T20:39:11Z |
| publishDate | 2019-01-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | BioImpacts |
| spelling | doaj.art-3e6907600340413bb824c4301d79604a2022-12-21T22:48:18ZengTabriz University of Medical SciencesBioImpacts2228-56602228-56522019-01-0191576310.15171/bi.2019.07bi-17634In vitro study of the anticancer activity of various doxorubicin-containing dispersionsTatiana Matvienko0Viktoriya Sokolova1Svitlana Prylutska2Yuliia Harahuts3Nataliya Kutsevol4Viktor Kostjukov5Maxim Evstigneev6Yuriy Prylutskyy7Matthias Epple8Uwe Ritter9Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineInstitute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, University Str., 5-7, 45117 Essen, GermanTaras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineTaras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineTaras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineDepartment of Physics, Sevastopol State University, Universitetskaya Str., 33, 299053 Sevastopol, CrimeaDepartment of Physics, Sevastopol State University, Universitetskaya Str., 33, 299053 Sevastopol, CrimeaTaras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, UkraineInstitute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, University Str., 5-7, 45117 Essen, GermanTechnical University Ilmenau, Institute of Chemistry and Biotechnology, Weimarer Str., 25, 98693 Ilmenau, GermanyIntroduction: The aim of this research was to study the impact of various doxorubicin (Dox)-containing nanofluids, e.g. singlewalled carbon nanotube (SWCNT)+Dox, graphene oxide (GO)+Dox and DextranPNIPAM (copolymer)+Dox mixtures on HeLa cells (human transformed cervix epithelial cells, as a model for cancer cells) depending on their concentration. Methods: Structural analysis of GO+Dox complex was accomplished using Hartree-Fock level of theory in 6-31G** basis set in Gaussian. Dynamic light scattering (DLS), zeta-potential, scanning electron microscopy and confocal laser scanning microscopy were used. The cell viability was analyzed by the MTT assay. Results: The viability of HeLa cells was studied with the MTT assay after the incubation with various Dox-containing dispersions depending on their concentration. The size of the particles was determined by DLS. The morphology of the nanoparticles (NPs) was studied by scanning electron microscopy and their uptake into cells was visualized by confocal laser scanning microscopy. It was found that the Dextran-PNIPAM+Dox nanofluid in contrast to Dox alone showed higher toxicity towards HeLa cells up to 80% after 24 hours of incubation, whereas the SWCNT+Dox and GO+Dox nanofluids at the same concentrations protected cells from Dox. Conclusion: The importance of Dextran-PNIPAM copolymer as a universal platform for drug delivery was established, and the huge potential of Dextran-PNIPAM+Dox NPs as novel anticancer agents was noted. Based on the in vitro study of the SWCNT+Dox and GO+Dox nanofluids, it was concluded that SWCNT and GO NPs can be effective cytoprotectors against the highly toxic drugs.https://bi.tbzmed.ac.ir/PDF/bi-9-57.pdfcytotoxicitydextran-pnipam copolymerdoxorubicingraphene oxidehela cellssingle-walled carbon nanotube |
| spellingShingle | Tatiana Matvienko Viktoriya Sokolova Svitlana Prylutska Yuliia Harahuts Nataliya Kutsevol Viktor Kostjukov Maxim Evstigneev Yuriy Prylutskyy Matthias Epple Uwe Ritter In vitro study of the anticancer activity of various doxorubicin-containing dispersions BioImpacts cytotoxicity dextran-pnipam copolymer doxorubicin graphene oxide hela cells single-walled carbon nanotube |
| title | In vitro study of the anticancer activity of various doxorubicin-containing dispersions |
| title_full | In vitro study of the anticancer activity of various doxorubicin-containing dispersions |
| title_fullStr | In vitro study of the anticancer activity of various doxorubicin-containing dispersions |
| title_full_unstemmed | In vitro study of the anticancer activity of various doxorubicin-containing dispersions |
| title_short | In vitro study of the anticancer activity of various doxorubicin-containing dispersions |
| title_sort | in vitro study of the anticancer activity of various doxorubicin containing dispersions |
| topic | cytotoxicity dextran-pnipam copolymer doxorubicin graphene oxide hela cells single-walled carbon nanotube |
| url | https://bi.tbzmed.ac.ir/PDF/bi-9-57.pdf |
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