Phytochemical Characterization and Efficacy of <em>Artemisia judaica</em> Extract Loaded Chitosan Nanoparticles as Inhibitors of Cancer Proliferation and Microbial Growth
Despite the advanced development in the field of drug discovery and design, fighting infectious and non-infectious diseases remains a major worldwide heath challenge due to the limited activity of currently used drugs. Nevertheless, in recent years, the approach of designing nanoparticles for therap...
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MDPI AG
2023-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/2/391 |
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| author | Husam Qanash Abdulrahman S. Bazaid Abdu Aldarhami Bandar Alharbi Majed N. Almashjary Mohannad S. Hazzazi Hashim R. Felemban Tarek M. Abdelghany |
| author_facet | Husam Qanash Abdulrahman S. Bazaid Abdu Aldarhami Bandar Alharbi Majed N. Almashjary Mohannad S. Hazzazi Hashim R. Felemban Tarek M. Abdelghany |
| author_sort | Husam Qanash |
| collection | DOAJ |
| description | Despite the advanced development in the field of drug discovery and design, fighting infectious and non-infectious diseases remains a major worldwide heath challenge due to the limited activity of currently used drugs. Nevertheless, in recent years, the approach of designing nanoparticles for therapeutic applications has gained more interest and promise for future use. Thus, the current study is focused on the evaluation of <i>A. judaica</i> extract and chitosan nanoparticles loaded extract (CNPsLE) for potential antimicrobial and anticancer activities. The HPLC analysis of the extract has shown the presence of various phenolic and flavonoid compounds, including kaempferol (3916.34 µg/mL), apigenin (3794.32 µg/mL), chlorogenic acid (1089.58 µg/mL), quercetin (714.97 µg/mL), vanillin (691.55 µg/mL), naringenin (202.14 µg/mL), and rutin (55.64 µg/mL). The extract alone showed higher MIC values against <i>B. subtilis</i>, <i>E. coli</i>, <i>S. aureus</i>, <i>K. pneumonia</i>, and <i>C. albicans</i> (62.5, 15.65, 15.62, 31.25, and 31.25 µg/mL, respectively), whereas lower MIC values were observed when the extract was combined with CNPsLE (0.97, 1.95, 3.9, 4.1, and 15.62 µg/mL, respectively). The extract exhibited low cytotoxicity against normal Vero cells with IC<sub>50</sub> 173.74 µg/mL in comparison with the cytotoxicity of the CNPsLE (IC<sub>50</sub>, 73.89 µg/mL). However, CNPsLE showed more selective toxicity against the human prostate cancer cell line (PC3) with IC<sub>50</sub> of 20.8 µg/mL than the extract alone with 76.09 µg/mL. In the docking experiments, kaempferol and apigenin were revealed to be suitable inhibitors for prostate cancer (2Q7L). Overall, the obtained data highlighted the promising potential therapeutic use of CNPsLE as an anticancer and antimicrobial agent. |
| first_indexed | 2024-03-09T11:23:13Z |
| format | Article |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-09T11:23:13Z |
| publishDate | 2023-01-01 |
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| series | Polymers |
| spelling | doaj.art-3e6949576d6441f790b2a1933c47f37b2023-12-01T00:09:17ZengMDPI AGPolymers2073-43602023-01-0115239110.3390/polym15020391Phytochemical Characterization and Efficacy of <em>Artemisia judaica</em> Extract Loaded Chitosan Nanoparticles as Inhibitors of Cancer Proliferation and Microbial GrowthHusam Qanash0Abdulrahman S. Bazaid1Abdu Aldarhami2Bandar Alharbi3Majed N. Almashjary4Mohannad S. Hazzazi5Hashim R. Felemban6Tarek M. Abdelghany7Department of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha’il, Hail 55476, Saudi ArabiaDepartment of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha’il, Hail 55476, Saudi ArabiaDepartment of Medical Microbiology, Qunfudah Faculty of Medicine, Umm Al-Qura University, Al-Qunfudah 21961, Saudi ArabiaDepartment of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha’il, Hail 55476, Saudi ArabiaDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 22254, Saudi ArabiaDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 22254, Saudi ArabiaDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 22254, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 71524, EgyptDespite the advanced development in the field of drug discovery and design, fighting infectious and non-infectious diseases remains a major worldwide heath challenge due to the limited activity of currently used drugs. Nevertheless, in recent years, the approach of designing nanoparticles for therapeutic applications has gained more interest and promise for future use. Thus, the current study is focused on the evaluation of <i>A. judaica</i> extract and chitosan nanoparticles loaded extract (CNPsLE) for potential antimicrobial and anticancer activities. The HPLC analysis of the extract has shown the presence of various phenolic and flavonoid compounds, including kaempferol (3916.34 µg/mL), apigenin (3794.32 µg/mL), chlorogenic acid (1089.58 µg/mL), quercetin (714.97 µg/mL), vanillin (691.55 µg/mL), naringenin (202.14 µg/mL), and rutin (55.64 µg/mL). The extract alone showed higher MIC values against <i>B. subtilis</i>, <i>E. coli</i>, <i>S. aureus</i>, <i>K. pneumonia</i>, and <i>C. albicans</i> (62.5, 15.65, 15.62, 31.25, and 31.25 µg/mL, respectively), whereas lower MIC values were observed when the extract was combined with CNPsLE (0.97, 1.95, 3.9, 4.1, and 15.62 µg/mL, respectively). The extract exhibited low cytotoxicity against normal Vero cells with IC<sub>50</sub> 173.74 µg/mL in comparison with the cytotoxicity of the CNPsLE (IC<sub>50</sub>, 73.89 µg/mL). However, CNPsLE showed more selective toxicity against the human prostate cancer cell line (PC3) with IC<sub>50</sub> of 20.8 µg/mL than the extract alone with 76.09 µg/mL. In the docking experiments, kaempferol and apigenin were revealed to be suitable inhibitors for prostate cancer (2Q7L). Overall, the obtained data highlighted the promising potential therapeutic use of CNPsLE as an anticancer and antimicrobial agent.https://www.mdpi.com/2073-4360/15/2/391Artemisiachitosan nanoparticlesanticancerantimicrobialbio-efficacy |
| spellingShingle | Husam Qanash Abdulrahman S. Bazaid Abdu Aldarhami Bandar Alharbi Majed N. Almashjary Mohannad S. Hazzazi Hashim R. Felemban Tarek M. Abdelghany Phytochemical Characterization and Efficacy of <em>Artemisia judaica</em> Extract Loaded Chitosan Nanoparticles as Inhibitors of Cancer Proliferation and Microbial Growth Polymers Artemisia chitosan nanoparticles anticancer antimicrobial bio-efficacy |
| title | Phytochemical Characterization and Efficacy of <em>Artemisia judaica</em> Extract Loaded Chitosan Nanoparticles as Inhibitors of Cancer Proliferation and Microbial Growth |
| title_full | Phytochemical Characterization and Efficacy of <em>Artemisia judaica</em> Extract Loaded Chitosan Nanoparticles as Inhibitors of Cancer Proliferation and Microbial Growth |
| title_fullStr | Phytochemical Characterization and Efficacy of <em>Artemisia judaica</em> Extract Loaded Chitosan Nanoparticles as Inhibitors of Cancer Proliferation and Microbial Growth |
| title_full_unstemmed | Phytochemical Characterization and Efficacy of <em>Artemisia judaica</em> Extract Loaded Chitosan Nanoparticles as Inhibitors of Cancer Proliferation and Microbial Growth |
| title_short | Phytochemical Characterization and Efficacy of <em>Artemisia judaica</em> Extract Loaded Chitosan Nanoparticles as Inhibitors of Cancer Proliferation and Microbial Growth |
| title_sort | phytochemical characterization and efficacy of em artemisia judaica em extract loaded chitosan nanoparticles as inhibitors of cancer proliferation and microbial growth |
| topic | Artemisia chitosan nanoparticles anticancer antimicrobial bio-efficacy |
| url | https://www.mdpi.com/2073-4360/15/2/391 |
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