Oxaliplatin Promotes Apoptosis of Gastric Cancer Cell Line SGC-7901 by Regulating miRNA-7-5p/RAF-1
Objective To investigate how oxaliplatin regulates the MAPK pathway and inhibits the proliferation of gastric cancer cells. Methods From NCBI, TargetScan, StarBase and miRBase databases were used to perform GO analysis and KEGG pathway enrichment in DAVID analysis, to find related miRNAs and predict...
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| Format: | Article |
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Magazine House of Cancer Research on Prevention and Treatment
2020-03-01
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| Series: | Zhongliu Fangzhi Yanjiu |
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| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.0695.htm |
| _version_ | 1811196627734495232 |
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| author | GU Zehui REN Liqun LI Qi WANG Yadi TAN Qi LOU Xiaoqian CHEN Suxian |
| author_facet | GU Zehui REN Liqun LI Qi WANG Yadi TAN Qi LOU Xiaoqian CHEN Suxian |
| author_sort | GU Zehui |
| collection | DOAJ |
| description | Objective To investigate how oxaliplatin regulates the MAPK pathway and inhibits the proliferation of gastric cancer cells. Methods From NCBI, TargetScan, StarBase and miRBase databases were used to perform GO analysis and KEGG pathway enrichment in DAVID analysis, to find related miRNAs and predict their target genes. The proliferation, cell cycle, invasion and protein expression of SGC-7901 cells were analyzed by Real-time PCR, MTT, Hoechst33258, flow cytometry, scratch assay and Western blot, respectively. Results miR-7-5p expression was significantly downregulated in gastric cancer cells. RAF1 and miR-7-5p were co-targeted. The apoptosis of SGC-7901 cells was promoted, the percentage of G1 phase cells and the expression of caspase3 and caspase9 were increased, while the percentage of G2 phase cells and the ratio of Bcl-2/Bax protein were decreased in miR-7-5p mimics and oxaliplatin (OXA) groups(P < 0.05). Conclusion Both overexpression of miR-7-5p and oxaliplatin could promote the apoptosis of gastric cancer SGC-7901 cells, suggesting that oxaliplatin may promote the apoptosis of SGC-7901 cells and reduce the rates of invasion and migration by up-regulating miRNA-7-5p. |
| first_indexed | 2024-04-12T01:02:03Z |
| format | Article |
| id | doaj.art-3e6ac82945b24029b9ca53270057dbac |
| institution | Directory Open Access Journal |
| issn | 1000-8578 1000-8578 |
| language | zho |
| last_indexed | 2024-04-12T01:02:03Z |
| publishDate | 2020-03-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-3e6ac82945b24029b9ca53270057dbac2022-12-22T03:54:24ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782020-03-0147317518010.3971/j.issn.1000-8578.2020.19.06958578.2020.19.0695Oxaliplatin Promotes Apoptosis of Gastric Cancer Cell Line SGC-7901 by Regulating miRNA-7-5p/RAF-1GU Zehui0REN Liqun1LI Qi2WANG Yadi3TAN Qi4LOU Xiaoqian5CHEN Suxian6Department of Pathology and Pathophysiology, Jinzhou Medical University, Jinzhou 121000, ChinaDepartment of Experimental Pharmacology and Toxicology, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaDepartment of Pathology, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, ChinaDepartment of Pathology, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, ChinaDepartment of Pathology and Pathophysiology, Jinzhou Medical University, Jinzhou 121000, ChinaDepartment of Experimental Pharmacology and Toxicology, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, ChinaDepartment of Pathology, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou 121000, ChinaObjective To investigate how oxaliplatin regulates the MAPK pathway and inhibits the proliferation of gastric cancer cells. Methods From NCBI, TargetScan, StarBase and miRBase databases were used to perform GO analysis and KEGG pathway enrichment in DAVID analysis, to find related miRNAs and predict their target genes. The proliferation, cell cycle, invasion and protein expression of SGC-7901 cells were analyzed by Real-time PCR, MTT, Hoechst33258, flow cytometry, scratch assay and Western blot, respectively. Results miR-7-5p expression was significantly downregulated in gastric cancer cells. RAF1 and miR-7-5p were co-targeted. The apoptosis of SGC-7901 cells was promoted, the percentage of G1 phase cells and the expression of caspase3 and caspase9 were increased, while the percentage of G2 phase cells and the ratio of Bcl-2/Bax protein were decreased in miR-7-5p mimics and oxaliplatin (OXA) groups(P < 0.05). Conclusion Both overexpression of miR-7-5p and oxaliplatin could promote the apoptosis of gastric cancer SGC-7901 cells, suggesting that oxaliplatin may promote the apoptosis of SGC-7901 cells and reduce the rates of invasion and migration by up-regulating miRNA-7-5p.http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.0695.htmmir-7-5poxaliplatingastric cancerapoptosisinvasion |
| spellingShingle | GU Zehui REN Liqun LI Qi WANG Yadi TAN Qi LOU Xiaoqian CHEN Suxian Oxaliplatin Promotes Apoptosis of Gastric Cancer Cell Line SGC-7901 by Regulating miRNA-7-5p/RAF-1 Zhongliu Fangzhi Yanjiu mir-7-5p oxaliplatin gastric cancer apoptosis invasion |
| title | Oxaliplatin Promotes Apoptosis of Gastric Cancer Cell Line SGC-7901 by Regulating miRNA-7-5p/RAF-1 |
| title_full | Oxaliplatin Promotes Apoptosis of Gastric Cancer Cell Line SGC-7901 by Regulating miRNA-7-5p/RAF-1 |
| title_fullStr | Oxaliplatin Promotes Apoptosis of Gastric Cancer Cell Line SGC-7901 by Regulating miRNA-7-5p/RAF-1 |
| title_full_unstemmed | Oxaliplatin Promotes Apoptosis of Gastric Cancer Cell Line SGC-7901 by Regulating miRNA-7-5p/RAF-1 |
| title_short | Oxaliplatin Promotes Apoptosis of Gastric Cancer Cell Line SGC-7901 by Regulating miRNA-7-5p/RAF-1 |
| title_sort | oxaliplatin promotes apoptosis of gastric cancer cell line sgc 7901 by regulating mirna 7 5p raf 1 |
| topic | mir-7-5p oxaliplatin gastric cancer apoptosis invasion |
| url | http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.0695.htm |
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