Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility
Background and Aim: Fertility is crucial for enhancing the efficiency of livestock production, as it directly impacts the reproductive rates. A comprehensive understanding of the relationship between sperm quality and embryo development is key to optimizing reproductive outcomes and improving the qu...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2024-03-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.17/March-2024/11.pdf |
| _version_ | 1797253762880897024 |
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| author | Hasbi Hasbi Hikmayani Iskandar Herry Sonjaya Bambang Purwantara Raden Iis Arifiantini Muhammad Agil Berlin Pandapotan Pardede Suyadi Suyadi Wike Andre Septian Daud Samsudewa Erni Damayanti Tulus Maulana Syahruddin Said |
| author_facet | Hasbi Hasbi Hikmayani Iskandar Herry Sonjaya Bambang Purwantara Raden Iis Arifiantini Muhammad Agil Berlin Pandapotan Pardede Suyadi Suyadi Wike Andre Septian Daud Samsudewa Erni Damayanti Tulus Maulana Syahruddin Said |
| author_sort | Hasbi Hasbi |
| collection | DOAJ |
| description | Background and Aim: Fertility is crucial for enhancing the efficiency of livestock production, as it directly impacts the reproductive rates. A comprehensive understanding of the relationship between sperm quality and embryo development is key to optimizing reproductive outcomes and improving the quality of livestock. This study analyzed the developmental competence of in vitro embryos recovered from Bali cattle with normal or poor sperm motility.
Materials and Methods: Nine bulls with normal fresh semen (NFS) or poor fresh semen (PFS) motility were ejaculated for semen. Semen ejaculates, including volume, motility, and sperm concentration, were evaluated immediately after collection to measure the quality of the fresh semen. Frozen semen was evaluated using computer-assisted semen analysis (CASA) for motility, progressive sperm motility, distance curve path, distance curve linear, distance straight line, average path velocity, curvilinear velocity, linear velocity, straightness (STR), linearity of forward progression (LIN), wobble, and average lateral head displacement (ALH). Bull groups were used to determine in vitro embryo cleavage ability after fertilization of Bali cattle. Ovaries of Bali cattle were collected by slicing, and only cytoplasmic oocytes with compact cumulus cells were used in this study. The oocytes were matured, and in vitro fertilization was performed using fertilization media with a final sperm concentration of 1.5 × 106 spermatozoa/mL. After 48 h, the embryo cleavage ability of the cultured oocytes was evaluated.
Results: There were significant differences in motility values between the NFS and PFS groups; however, there were no significant differences in the volume or sperm concentration. There was a significant difference in the LIN value between the groups but no significant differences in other CASA parameters. There were no significant differences in the cleavage rate and morula between the groups, but a positive correlation was observed between the cleavage rate and the morula and between the morula and ALH. A significant negative correlation was observed between the cleavage rate and STR and between the morula and STR; no significant differences were observed for other variables.
Conclusion: Despite variations in sperm characteristics, both normal and poor sperm motility demonstrated comparable in vitro embryonic development competence. These findings provide important insights into the fertility potential of Bali bulls, providing valuable information that can enhance selection strategies to improve the quality of livestock production. |
| first_indexed | 2024-04-24T21:39:13Z |
| format | Article |
| id | doaj.art-3e6ba59aa0424bc297d889854bbd76b0 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-24T21:39:13Z |
| publishDate | 2024-03-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-3e6ba59aa0424bc297d889854bbd76b02024-03-21T11:39:35ZengVeterinary WorldVeterinary World0972-89882231-09162024-03-0117359360110.14202/vetworld.2024.593-601Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motilityHasbi Hasbi0https://orcid.org/0000-0002-6069-2171Hikmayani Iskandar1https://orcid.org/0000-0002-5786-5094Herry Sonjaya2https://orcid.org/0000-0002-8545-4110Bambang Purwantara3https://orcid.org/0000-0002-6652-4902Raden Iis Arifiantini4https://orcid.org/0000-0002-7478-9433Muhammad Agil5https://orcid.org/0000-0003-3591-4219Berlin Pandapotan Pardede6https://orcid.org/0000-0003-3786-2432Suyadi Suyadi7https://orcid.org/0000-0003-3509-1064Wike Andre Septian8https://orcid.org/0000-0002-3883-6730Daud Samsudewa9https://orcid.org/0000-0001-6007-6355Erni Damayanti10https://orcid.org/0000-0003-3028-2267Tulus Maulana11https://orcid.org/0000-0002-5578-3992Syahruddin Said12https://orcid.org/0000-0001-8681-4985Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, 90245, Indonesia.Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, 16914, Indonesia.Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, 90245, Indonesia.Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia.Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia.Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia.Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, 16914, Indonesia. Faculty of Animal Science, Brawijaya University, Malang, 65145, Indonesia.Faculty of Animal Science, Brawijaya University, Malang, 65145, Indonesia.Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, 50275, Indonesia.Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, 16914, Indonesia.Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, 16914, Indonesia.Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, 16914, Indonesia.Background and Aim: Fertility is crucial for enhancing the efficiency of livestock production, as it directly impacts the reproductive rates. A comprehensive understanding of the relationship between sperm quality and embryo development is key to optimizing reproductive outcomes and improving the quality of livestock. This study analyzed the developmental competence of in vitro embryos recovered from Bali cattle with normal or poor sperm motility. Materials and Methods: Nine bulls with normal fresh semen (NFS) or poor fresh semen (PFS) motility were ejaculated for semen. Semen ejaculates, including volume, motility, and sperm concentration, were evaluated immediately after collection to measure the quality of the fresh semen. Frozen semen was evaluated using computer-assisted semen analysis (CASA) for motility, progressive sperm motility, distance curve path, distance curve linear, distance straight line, average path velocity, curvilinear velocity, linear velocity, straightness (STR), linearity of forward progression (LIN), wobble, and average lateral head displacement (ALH). Bull groups were used to determine in vitro embryo cleavage ability after fertilization of Bali cattle. Ovaries of Bali cattle were collected by slicing, and only cytoplasmic oocytes with compact cumulus cells were used in this study. The oocytes were matured, and in vitro fertilization was performed using fertilization media with a final sperm concentration of 1.5 × 106 spermatozoa/mL. After 48 h, the embryo cleavage ability of the cultured oocytes was evaluated. Results: There were significant differences in motility values between the NFS and PFS groups; however, there were no significant differences in the volume or sperm concentration. There was a significant difference in the LIN value between the groups but no significant differences in other CASA parameters. There were no significant differences in the cleavage rate and morula between the groups, but a positive correlation was observed between the cleavage rate and the morula and between the morula and ALH. A significant negative correlation was observed between the cleavage rate and STR and between the morula and STR; no significant differences were observed for other variables. Conclusion: Despite variations in sperm characteristics, both normal and poor sperm motility demonstrated comparable in vitro embryonic development competence. These findings provide important insights into the fertility potential of Bali bulls, providing valuable information that can enhance selection strategies to improve the quality of livestock production.https://www.veterinaryworld.org/Vol.17/March-2024/11.pdfbali cattleembryosin vitro semen quality |
| spellingShingle | Hasbi Hasbi Hikmayani Iskandar Herry Sonjaya Bambang Purwantara Raden Iis Arifiantini Muhammad Agil Berlin Pandapotan Pardede Suyadi Suyadi Wike Andre Septian Daud Samsudewa Erni Damayanti Tulus Maulana Syahruddin Said Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility Veterinary World bali cattle embryos in vitro semen quality |
| title | Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility |
| title_full | Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility |
| title_fullStr | Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility |
| title_full_unstemmed | Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility |
| title_short | Comparative developmental competence of in vitro embryos recovered from Bali cattle with normal and poor sperm motility |
| title_sort | comparative developmental competence of in vitro embryos recovered from bali cattle with normal and poor sperm motility |
| topic | bali cattle embryos in vitro semen quality |
| url | https://www.veterinaryworld.org/Vol.17/March-2024/11.pdf |
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