Prevalence, Tetracycline Resistance and Tet(O) Gene Identification in Pathogenic <i>Campylobacter</i> Strains Isolated from Chickens in Retail Markets of Lima, Peru
Background: In this study, we aimed to estimate the prevalence, tetracycline resistance and presence of Tet(O) in <i>Campylobacter</i> strains isolated from chicken in markets of Lima, Peru. Methods: A total of 250 chicken samples were obtained from traditional markets (skin, <i>n&...
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MDPI AG
2022-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/11/1580 |
| _version_ | 1797469333410021376 |
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| author | Christian Benites Diego Anampa Domingo Torres Ivette Avalos Miguel Rojas Carlos Conte César Lázaro |
| author_facet | Christian Benites Diego Anampa Domingo Torres Ivette Avalos Miguel Rojas Carlos Conte César Lázaro |
| author_sort | Christian Benites |
| collection | DOAJ |
| description | Background: In this study, we aimed to estimate the prevalence, tetracycline resistance and presence of Tet(O) in <i>Campylobacter</i> strains isolated from chicken in markets of Lima, Peru. Methods: A total of 250 chicken samples were obtained from traditional markets (skin, <i>n</i> = 120) and supermarkets (meat, <i>n</i> = 130). Samples were subjected to microbiological assays for identification of <i>Campylobacter</i> spp. according to ISO 10272-2017, and the isolates were then submitted to species identification by PCR. Phenotypic resistance to tetracyclines was assessed by the Kirby–Bauer test, and the presence of the Tet(O) gene was determined by PCR. Results: A significantly higher prevalence (<i>p</i> < 0.0001) of <i>Campylobacter coli</i> in skin samples from traditional markets (97.5%) than in meat samples from supermarkets (36.2%) was observed. On the other hand, <i>Campylobacter jejuni</i> was confirmed only in 3.1% of meat samples. All <i>Campylobacter</i> species isolated from skin and meat samples were phenotypically resistant to tetracyclines; however, the presence of the Tet(O) gene in <i>C. coli</i> was identified in 76.9% and 66.0% of skin and meat samples, no significant statistical difference (<i>p</i> = 0.1488) was found between these prevalence. All <i>C. jejuni</i> isolated from chicken meat samples from supermarkets were positive for Tet(O) gene. Conclusions: This study confirms the high prevalence of <i>C. coli</i> isolated from chicken sold in traditional markets and supermarkets in Lima, Peru, and in more than 70% of these strains, phenotypic resistance to tetracyclines could be linked with expression of the Tet(O) gene. It is necessary to evaluate other genes involved in resistance to tetracyclines and other groups of antibiotics in campylobacter strains isolated from chicken meat. |
| first_indexed | 2024-03-09T19:18:56Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T19:18:56Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-3e6bc46550284744aa61116b1cdffee72023-11-24T03:28:44ZengMDPI AGAntibiotics2079-63822022-11-011111158010.3390/antibiotics11111580Prevalence, Tetracycline Resistance and Tet(O) Gene Identification in Pathogenic <i>Campylobacter</i> Strains Isolated from Chickens in Retail Markets of Lima, PeruChristian Benites0Diego Anampa1Domingo Torres2Ivette Avalos3Miguel Rojas4Carlos Conte5César Lázaro6Laboratorio de Farmacología y Toxicología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Apartado 03-5137, PeruLaboratorio de Farmacología y Toxicología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Apartado 03-5137, PeruLaboratorio de Farmacología y Toxicología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Apartado 03-5137, PeruLaboratorio de Inmunología, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Apartado 03-5137, PeruLaboratorio de Inmunología, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Apartado 03-5137, PeruInstituto de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos, 149, Cidade Universitária, Rio de Janeiro 21941-909, BrazilLaboratorio de Farmacología y Toxicología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Apartado 03-5137, PeruBackground: In this study, we aimed to estimate the prevalence, tetracycline resistance and presence of Tet(O) in <i>Campylobacter</i> strains isolated from chicken in markets of Lima, Peru. Methods: A total of 250 chicken samples were obtained from traditional markets (skin, <i>n</i> = 120) and supermarkets (meat, <i>n</i> = 130). Samples were subjected to microbiological assays for identification of <i>Campylobacter</i> spp. according to ISO 10272-2017, and the isolates were then submitted to species identification by PCR. Phenotypic resistance to tetracyclines was assessed by the Kirby–Bauer test, and the presence of the Tet(O) gene was determined by PCR. Results: A significantly higher prevalence (<i>p</i> < 0.0001) of <i>Campylobacter coli</i> in skin samples from traditional markets (97.5%) than in meat samples from supermarkets (36.2%) was observed. On the other hand, <i>Campylobacter jejuni</i> was confirmed only in 3.1% of meat samples. All <i>Campylobacter</i> species isolated from skin and meat samples were phenotypically resistant to tetracyclines; however, the presence of the Tet(O) gene in <i>C. coli</i> was identified in 76.9% and 66.0% of skin and meat samples, no significant statistical difference (<i>p</i> = 0.1488) was found between these prevalence. All <i>C. jejuni</i> isolated from chicken meat samples from supermarkets were positive for Tet(O) gene. Conclusions: This study confirms the high prevalence of <i>C. coli</i> isolated from chicken sold in traditional markets and supermarkets in Lima, Peru, and in more than 70% of these strains, phenotypic resistance to tetracyclines could be linked with expression of the Tet(O) gene. It is necessary to evaluate other genes involved in resistance to tetracyclines and other groups of antibiotics in campylobacter strains isolated from chicken meat.https://www.mdpi.com/2079-6382/11/11/1580antibiotic resistance<i>Campylobacter</i>chickentetracyclines |
| spellingShingle | Christian Benites Diego Anampa Domingo Torres Ivette Avalos Miguel Rojas Carlos Conte César Lázaro Prevalence, Tetracycline Resistance and Tet(O) Gene Identification in Pathogenic <i>Campylobacter</i> Strains Isolated from Chickens in Retail Markets of Lima, Peru Antibiotics antibiotic resistance <i>Campylobacter</i> chicken tetracyclines |
| title | Prevalence, Tetracycline Resistance and Tet(O) Gene Identification in Pathogenic <i>Campylobacter</i> Strains Isolated from Chickens in Retail Markets of Lima, Peru |
| title_full | Prevalence, Tetracycline Resistance and Tet(O) Gene Identification in Pathogenic <i>Campylobacter</i> Strains Isolated from Chickens in Retail Markets of Lima, Peru |
| title_fullStr | Prevalence, Tetracycline Resistance and Tet(O) Gene Identification in Pathogenic <i>Campylobacter</i> Strains Isolated from Chickens in Retail Markets of Lima, Peru |
| title_full_unstemmed | Prevalence, Tetracycline Resistance and Tet(O) Gene Identification in Pathogenic <i>Campylobacter</i> Strains Isolated from Chickens in Retail Markets of Lima, Peru |
| title_short | Prevalence, Tetracycline Resistance and Tet(O) Gene Identification in Pathogenic <i>Campylobacter</i> Strains Isolated from Chickens in Retail Markets of Lima, Peru |
| title_sort | prevalence tetracycline resistance and tet o gene identification in pathogenic i campylobacter i strains isolated from chickens in retail markets of lima peru |
| topic | antibiotic resistance <i>Campylobacter</i> chicken tetracyclines |
| url | https://www.mdpi.com/2079-6382/11/11/1580 |
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