Green Synthesis and Characterization of Silver Nanoparticles Using <i>Flaxseed</i> Extract and Evaluation of Their Antibacterial and Antioxidant Activities
Bioactive plant chemicals are considered to be rich and useful for creating nanomaterials. The current work investigated the biosynthesis of silver nanoparticles (AgNPs) using ethanolic <i>flaxseed</i> extract as an efficient reducing factor. The production of AgNPs was verified by color...
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MDPI AG
2023-02-01
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| Series: | Applied Sciences |
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| author | Azalldeen Kazal Alzubaidi Wasan J. Al-Kaabi Amer Al Ali Salim Albukhaty Hassan Al-Karagoly Ghassan M. Sulaiman Mohammed Asiri Yasmina Khane |
| author_facet | Azalldeen Kazal Alzubaidi Wasan J. Al-Kaabi Amer Al Ali Salim Albukhaty Hassan Al-Karagoly Ghassan M. Sulaiman Mohammed Asiri Yasmina Khane |
| author_sort | Azalldeen Kazal Alzubaidi |
| collection | DOAJ |
| description | Bioactive plant chemicals are considered to be rich and useful for creating nanomaterials. The current work investigated the biosynthesis of silver nanoparticles (AgNPs) using ethanolic <i>flaxseed</i> extract as an efficient reducing factor. The production of AgNPs was verified by color-shifting observation of the mixture of silver nitrate (AgNO<sub>3</sub>) from yellow to a reddish suspension after the addition of the extract and by evaluating it by UV–visible inspection. Additionally, FTIR spectrum was used to support the identification of functional groups. The morphology and structure of AgNPs were assessed using scanning electron microscopy (SEM), and X-ray diffraction (XRD) examinations, which revealed spherical AgNPs with a diameter of 46.98 ± 12.45 nm and a crystalline structure. The zeta potential (ZP) and dynamic light scattering (DLS) measurements of AgNPs revealed values of −44.5 mV and 231.8 nm, respectively, suggesting appropriate physical stability. The antibacterial activity of AgNPs was investigated against <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, <i>Staphylococcus aureus</i>, and <i>Streptococcus pyogenes</i>, while the antioxidant effect was investigated using the DPPH technique. These obtained AgNPs could potentially be used as efficient antibacterial and antioxidant nanomaterials. |
| first_indexed | 2024-03-11T09:12:50Z |
| format | Article |
| id | doaj.art-3e6bcf71294c49d9a25a66b573cde33d |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T09:12:50Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-3e6bcf71294c49d9a25a66b573cde33d2023-11-16T18:52:03ZengMDPI AGApplied Sciences2076-34172023-02-01134218210.3390/app13042182Green Synthesis and Characterization of Silver Nanoparticles Using <i>Flaxseed</i> Extract and Evaluation of Their Antibacterial and Antioxidant ActivitiesAzalldeen Kazal Alzubaidi0Wasan J. Al-Kaabi1Amer Al Ali2Salim Albukhaty3Hassan Al-Karagoly4Ghassan M. Sulaiman5Mohammed Asiri6Yasmina Khane7College of Agriculture, University of Misan, Al-Amara 62001, IraqDepartment of Science, College of Basic Education, University of Misan, Maysan 62001, IraqDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, 255, Bisha 67714, Saudi ArabiaDepartment of Chemistry, College of Science, University of Misan, Maysan 62001, IraqDepartment of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah 58002, IraqDivision of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad 10066, IraqDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha 9088, Saudi ArabiaFaculté des sciences et technologies, Université de Ghardaia, BP 455, Ghardaïa 47000, AlgeriaBioactive plant chemicals are considered to be rich and useful for creating nanomaterials. The current work investigated the biosynthesis of silver nanoparticles (AgNPs) using ethanolic <i>flaxseed</i> extract as an efficient reducing factor. The production of AgNPs was verified by color-shifting observation of the mixture of silver nitrate (AgNO<sub>3</sub>) from yellow to a reddish suspension after the addition of the extract and by evaluating it by UV–visible inspection. Additionally, FTIR spectrum was used to support the identification of functional groups. The morphology and structure of AgNPs were assessed using scanning electron microscopy (SEM), and X-ray diffraction (XRD) examinations, which revealed spherical AgNPs with a diameter of 46.98 ± 12.45 nm and a crystalline structure. The zeta potential (ZP) and dynamic light scattering (DLS) measurements of AgNPs revealed values of −44.5 mV and 231.8 nm, respectively, suggesting appropriate physical stability. The antibacterial activity of AgNPs was investigated against <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, <i>Staphylococcus aureus</i>, and <i>Streptococcus pyogenes</i>, while the antioxidant effect was investigated using the DPPH technique. These obtained AgNPs could potentially be used as efficient antibacterial and antioxidant nanomaterials.https://www.mdpi.com/2076-3417/13/4/2182flaxseed extractgreen synthesis AgNPsantioxidant activityantimicrobial activity |
| spellingShingle | Azalldeen Kazal Alzubaidi Wasan J. Al-Kaabi Amer Al Ali Salim Albukhaty Hassan Al-Karagoly Ghassan M. Sulaiman Mohammed Asiri Yasmina Khane Green Synthesis and Characterization of Silver Nanoparticles Using <i>Flaxseed</i> Extract and Evaluation of Their Antibacterial and Antioxidant Activities Applied Sciences flaxseed extract green synthesis AgNPs antioxidant activity antimicrobial activity |
| title | Green Synthesis and Characterization of Silver Nanoparticles Using <i>Flaxseed</i> Extract and Evaluation of Their Antibacterial and Antioxidant Activities |
| title_full | Green Synthesis and Characterization of Silver Nanoparticles Using <i>Flaxseed</i> Extract and Evaluation of Their Antibacterial and Antioxidant Activities |
| title_fullStr | Green Synthesis and Characterization of Silver Nanoparticles Using <i>Flaxseed</i> Extract and Evaluation of Their Antibacterial and Antioxidant Activities |
| title_full_unstemmed | Green Synthesis and Characterization of Silver Nanoparticles Using <i>Flaxseed</i> Extract and Evaluation of Their Antibacterial and Antioxidant Activities |
| title_short | Green Synthesis and Characterization of Silver Nanoparticles Using <i>Flaxseed</i> Extract and Evaluation of Their Antibacterial and Antioxidant Activities |
| title_sort | green synthesis and characterization of silver nanoparticles using i flaxseed i extract and evaluation of their antibacterial and antioxidant activities |
| topic | flaxseed extract green synthesis AgNPs antioxidant activity antimicrobial activity |
| url | https://www.mdpi.com/2076-3417/13/4/2182 |
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