Cell Tracking by Magnetic Particle Imaging: Methodology for Labeling THP-1 Monocytes with Magnetic Nanoparticles for Cellular Imaging
Magnetic particle imaging (MPI) is a noninvasive tomographic imaging modality for the quantitative visualization of magnetic nanoparticles (MNPs) with high temporal and spatial resolution. The general capability of MPI for cell tracking (e.g., monitoring living cells labeled with MNPs) has successfu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/18/2892 |
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| author | Amani Remmo Norbert Löwa Olaf Kosch Dietmar Eberbeck Antje Ludwig Lena Kampen Cordula Grüttner Frank Wiekhorst |
| author_facet | Amani Remmo Norbert Löwa Olaf Kosch Dietmar Eberbeck Antje Ludwig Lena Kampen Cordula Grüttner Frank Wiekhorst |
| author_sort | Amani Remmo |
| collection | DOAJ |
| description | Magnetic particle imaging (MPI) is a noninvasive tomographic imaging modality for the quantitative visualization of magnetic nanoparticles (MNPs) with high temporal and spatial resolution. The general capability of MPI for cell tracking (e.g., monitoring living cells labeled with MNPs) has successfully been shown. MNPs in cell culture media are often subjected to structural and magnetic changes. In addition to the deteriorating reproducibility, this also complicates the systematic study of the relationship between the MNP properties and their cellular uptake for MPI. Here, we present a method for the preparation of magnetically labeled THP-1 (Tamm–Horsfall Protein-1) monocytes that are used in MPI cell tracking. The method development was performed using two different MPI tracers, which exhibited electrostatic and steric stabilizations, respectively. In the first step, the interaction between the MNPs and cell culture media was investigated and adjusted to ensure high structural and magnetic stability. Furthermore, the influences of the incubation time, MNP concentration used for cellular uptake, and individual preparation steps (e.g., the washing of cells) were systematically investigated. Finally, the success of the developed loading method was demonstrated by the MPI measurements. The presented systematic investigation of the factors that influence the MNP loading of cells will help to develop a reliable and reproducible method for MPI monocyte tracking for the early detection of inflammation in the future. |
| first_indexed | 2024-03-10T00:25:32Z |
| format | Article |
| id | doaj.art-3e6f877a94e04ee987026db05c79aad7 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:25:32Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3e6f877a94e04ee987026db05c79aad72023-11-23T15:33:59ZengMDPI AGCells2073-44092022-09-011118289210.3390/cells11182892Cell Tracking by Magnetic Particle Imaging: Methodology for Labeling THP-1 Monocytes with Magnetic Nanoparticles for Cellular ImagingAmani Remmo0Norbert Löwa1Olaf Kosch2Dietmar Eberbeck3Antje Ludwig4Lena Kampen5Cordula Grüttner6Frank Wiekhorst7Physikalisch-Technische Bundesanstalt, Abbestraße 2-12, 10587 Berlin, GermanyPhysikalisch-Technische Bundesanstalt, Abbestraße 2-12, 10587 Berlin, GermanyPhysikalisch-Technische Bundesanstalt, Abbestraße 2-12, 10587 Berlin, GermanyPhysikalisch-Technische Bundesanstalt, Abbestraße 2-12, 10587 Berlin, GermanyCharité, Center for Cardiovascular Research (CCR), Berlin, Hessische Straße 3-4, 10115 Berlin, GermanyCharité, Center for Cardiovascular Research (CCR), Berlin, Hessische Straße 3-4, 10115 Berlin, GermanyMicromod Partikeltechnologie GmbH, Schillingallee 68, 18057 Rostock, GermanyPhysikalisch-Technische Bundesanstalt, Abbestraße 2-12, 10587 Berlin, GermanyMagnetic particle imaging (MPI) is a noninvasive tomographic imaging modality for the quantitative visualization of magnetic nanoparticles (MNPs) with high temporal and spatial resolution. The general capability of MPI for cell tracking (e.g., monitoring living cells labeled with MNPs) has successfully been shown. MNPs in cell culture media are often subjected to structural and magnetic changes. In addition to the deteriorating reproducibility, this also complicates the systematic study of the relationship between the MNP properties and their cellular uptake for MPI. Here, we present a method for the preparation of magnetically labeled THP-1 (Tamm–Horsfall Protein-1) monocytes that are used in MPI cell tracking. The method development was performed using two different MPI tracers, which exhibited electrostatic and steric stabilizations, respectively. In the first step, the interaction between the MNPs and cell culture media was investigated and adjusted to ensure high structural and magnetic stability. Furthermore, the influences of the incubation time, MNP concentration used for cellular uptake, and individual preparation steps (e.g., the washing of cells) were systematically investigated. Finally, the success of the developed loading method was demonstrated by the MPI measurements. The presented systematic investigation of the factors that influence the MNP loading of cells will help to develop a reliable and reproducible method for MPI monocyte tracking for the early detection of inflammation in the future.https://www.mdpi.com/2073-4409/11/18/2892magnetic nanoparticlesmagnetic particle spectroscopymagnetic particle imagingcell trackingTHP-1 monocytesmicroscopy |
| spellingShingle | Amani Remmo Norbert Löwa Olaf Kosch Dietmar Eberbeck Antje Ludwig Lena Kampen Cordula Grüttner Frank Wiekhorst Cell Tracking by Magnetic Particle Imaging: Methodology for Labeling THP-1 Monocytes with Magnetic Nanoparticles for Cellular Imaging Cells magnetic nanoparticles magnetic particle spectroscopy magnetic particle imaging cell tracking THP-1 monocytes microscopy |
| title | Cell Tracking by Magnetic Particle Imaging: Methodology for Labeling THP-1 Monocytes with Magnetic Nanoparticles for Cellular Imaging |
| title_full | Cell Tracking by Magnetic Particle Imaging: Methodology for Labeling THP-1 Monocytes with Magnetic Nanoparticles for Cellular Imaging |
| title_fullStr | Cell Tracking by Magnetic Particle Imaging: Methodology for Labeling THP-1 Monocytes with Magnetic Nanoparticles for Cellular Imaging |
| title_full_unstemmed | Cell Tracking by Magnetic Particle Imaging: Methodology for Labeling THP-1 Monocytes with Magnetic Nanoparticles for Cellular Imaging |
| title_short | Cell Tracking by Magnetic Particle Imaging: Methodology for Labeling THP-1 Monocytes with Magnetic Nanoparticles for Cellular Imaging |
| title_sort | cell tracking by magnetic particle imaging methodology for labeling thp 1 monocytes with magnetic nanoparticles for cellular imaging |
| topic | magnetic nanoparticles magnetic particle spectroscopy magnetic particle imaging cell tracking THP-1 monocytes microscopy |
| url | https://www.mdpi.com/2073-4409/11/18/2892 |
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