Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells
The concept of hormesis describes a phenomenon of adaptive response to low-dose ionizing radiation (LDIR). Similarly, the concept of mitohormesis states that the adaptive program in mitochondria is activated in response to minor stress effects. The mechanisms of hormesis effects are not clear, but i...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/1/261 |
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| author | Svetlana V. Kostyuk Elena V. Proskurnina Marina S. Konkova Margarita S. Abramova Andrey A. Kalianov Elizaveta S. Ershova Vera L. Izhevskaya Sergey I. Kutsev Natalia N. Veiko |
| author_facet | Svetlana V. Kostyuk Elena V. Proskurnina Marina S. Konkova Margarita S. Abramova Andrey A. Kalianov Elizaveta S. Ershova Vera L. Izhevskaya Sergey I. Kutsev Natalia N. Veiko |
| author_sort | Svetlana V. Kostyuk |
| collection | DOAJ |
| description | The concept of hormesis describes a phenomenon of adaptive response to low-dose ionizing radiation (LDIR). Similarly, the concept of mitohormesis states that the adaptive program in mitochondria is activated in response to minor stress effects. The mechanisms of hormesis effects are not clear, but it is assumed that they can be mediated by reactive oxygen species. Here, we studied effects of LDIR on mitochondria in mesenchymal stem cells. We have found that X-ray radiation at a dose of 10 cGy as well as oxidized fragments of cell-free DNA (cfDNA) at a concentration of 50 ng/mL resulted in an increased expression of a large number of genes regulating the function of the mitochondrial respiratory chain complexes in human mesenchymal stem cells (MSC). Several genes remained upregulated within hours after the exposure. Both X-ray radiation and oxidized cfDNA resulted in upregulation of <i>FIS1</i> and <i>MFN1</i> genes, which regulated fusion and fission of mitochondria, within 3–24 h after the exposure. Three hours after the exposure, the number of copies of mitochondrial DNA in cells had increased. These findings support the hypothesis that assumes oxidized cell-free DNA as a mediator of MSC response to low doses of radiation. |
| first_indexed | 2024-03-10T03:39:25Z |
| format | Article |
| id | doaj.art-3e7017c2f715439aa28f8bcca805208e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:39:25Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3e7017c2f715439aa28f8bcca805208e2023-11-23T11:37:19ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0123126110.3390/ijms23010261Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem CellsSvetlana V. Kostyuk0Elena V. Proskurnina1Marina S. Konkova2Margarita S. Abramova3Andrey A. Kalianov4Elizaveta S. Ershova5Vera L. Izhevskaya6Sergey I. Kutsev7Natalia N. Veiko8Laboratory of Molecular Biology, Research Centre for Medical Genetics, 1 Moskvorechye St., 115522 Moscow, RussiaLaboratory of Molecular Biology, Research Centre for Medical Genetics, 1 Moskvorechye St., 115522 Moscow, RussiaLaboratory of Molecular Biology, Research Centre for Medical Genetics, 1 Moskvorechye St., 115522 Moscow, RussiaLaboratory of Molecular Biology, Research Centre for Medical Genetics, 1 Moskvorechye St., 115522 Moscow, RussiaLaboratory of Molecular Biology, Research Centre for Medical Genetics, 1 Moskvorechye St., 115522 Moscow, RussiaLaboratory of Molecular Biology, Research Centre for Medical Genetics, 1 Moskvorechye St., 115522 Moscow, RussiaLaboratory of Molecular Biology, Research Centre for Medical Genetics, 1 Moskvorechye St., 115522 Moscow, RussiaLaboratory of Molecular Biology, Research Centre for Medical Genetics, 1 Moskvorechye St., 115522 Moscow, RussiaLaboratory of Molecular Biology, Research Centre for Medical Genetics, 1 Moskvorechye St., 115522 Moscow, RussiaThe concept of hormesis describes a phenomenon of adaptive response to low-dose ionizing radiation (LDIR). Similarly, the concept of mitohormesis states that the adaptive program in mitochondria is activated in response to minor stress effects. The mechanisms of hormesis effects are not clear, but it is assumed that they can be mediated by reactive oxygen species. Here, we studied effects of LDIR on mitochondria in mesenchymal stem cells. We have found that X-ray radiation at a dose of 10 cGy as well as oxidized fragments of cell-free DNA (cfDNA) at a concentration of 50 ng/mL resulted in an increased expression of a large number of genes regulating the function of the mitochondrial respiratory chain complexes in human mesenchymal stem cells (MSC). Several genes remained upregulated within hours after the exposure. Both X-ray radiation and oxidized cfDNA resulted in upregulation of <i>FIS1</i> and <i>MFN1</i> genes, which regulated fusion and fission of mitochondria, within 3–24 h after the exposure. Three hours after the exposure, the number of copies of mitochondrial DNA in cells had increased. These findings support the hypothesis that assumes oxidized cell-free DNA as a mediator of MSC response to low doses of radiation.https://www.mdpi.com/1422-0067/23/1/261low-dose ionizing radiationmitohormesiscell-free DNAhuman mesenchymal stem cellsmitochondria |
| spellingShingle | Svetlana V. Kostyuk Elena V. Proskurnina Marina S. Konkova Margarita S. Abramova Andrey A. Kalianov Elizaveta S. Ershova Vera L. Izhevskaya Sergey I. Kutsev Natalia N. Veiko Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells International Journal of Molecular Sciences low-dose ionizing radiation mitohormesis cell-free DNA human mesenchymal stem cells mitochondria |
| title | Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells |
| title_full | Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells |
| title_fullStr | Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells |
| title_full_unstemmed | Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells |
| title_short | Effect of Low-Dose Ionizing Radiation on the Expression of Mitochondria-Related Genes in Human Mesenchymal Stem Cells |
| title_sort | effect of low dose ionizing radiation on the expression of mitochondria related genes in human mesenchymal stem cells |
| topic | low-dose ionizing radiation mitohormesis cell-free DNA human mesenchymal stem cells mitochondria |
| url | https://www.mdpi.com/1422-0067/23/1/261 |
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