Toxicity and Antioxidant Activity of Fullerenol C<sub>60,70</sub> with Low Number of Oxygen Substituents
Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/12/6382 |
| _version_ | 1797530107360837632 |
|---|---|
| author | Ekaterina S. Kovel Arina G. Kicheeva Natalia G. Vnukova Grigory N. Churilov Evsei A. Stepin Nadezhda S. Kudryasheva |
| author_facet | Ekaterina S. Kovel Arina G. Kicheeva Natalia G. Vnukova Grigory N. Churilov Evsei A. Stepin Nadezhda S. Kudryasheva |
| author_sort | Ekaterina S. Kovel |
| collection | DOAJ |
| description | Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene’s carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system. |
| first_indexed | 2024-03-10T10:24:14Z |
| format | Article |
| id | doaj.art-3e77a032620e4192b1f164796d152cb4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:24:14Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3e77a032620e4192b1f164796d152cb42023-11-22T00:08:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212638210.3390/ijms22126382Toxicity and Antioxidant Activity of Fullerenol C<sub>60,70</sub> with Low Number of Oxygen SubstituentsEkaterina S. Kovel0Arina G. Kicheeva1Natalia G. Vnukova2Grigory N. Churilov3Evsei A. Stepin4Nadezhda S. Kudryasheva5Institute of Biophysics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, RussiaFRC KSC SB RAS, 660036 Krasnoyarsk, RussiaInstitute of Physics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, RussiaInstitute of Physics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, RussiaInstitute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, FRC KSC SB RAS, 660036 Krasnoyarsk, RussiaFullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene’s carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system.https://www.mdpi.com/1422-0067/22/12/6382fullerenoltoxicityantioxidant activityreactive oxygen speciesbioluminescent assayhormesis |
| spellingShingle | Ekaterina S. Kovel Arina G. Kicheeva Natalia G. Vnukova Grigory N. Churilov Evsei A. Stepin Nadezhda S. Kudryasheva Toxicity and Antioxidant Activity of Fullerenol C<sub>60,70</sub> with Low Number of Oxygen Substituents International Journal of Molecular Sciences fullerenol toxicity antioxidant activity reactive oxygen species bioluminescent assay hormesis |
| title | Toxicity and Antioxidant Activity of Fullerenol C<sub>60,70</sub> with Low Number of Oxygen Substituents |
| title_full | Toxicity and Antioxidant Activity of Fullerenol C<sub>60,70</sub> with Low Number of Oxygen Substituents |
| title_fullStr | Toxicity and Antioxidant Activity of Fullerenol C<sub>60,70</sub> with Low Number of Oxygen Substituents |
| title_full_unstemmed | Toxicity and Antioxidant Activity of Fullerenol C<sub>60,70</sub> with Low Number of Oxygen Substituents |
| title_short | Toxicity and Antioxidant Activity of Fullerenol C<sub>60,70</sub> with Low Number of Oxygen Substituents |
| title_sort | toxicity and antioxidant activity of fullerenol c sub 60 70 sub with low number of oxygen substituents |
| topic | fullerenol toxicity antioxidant activity reactive oxygen species bioluminescent assay hormesis |
| url | https://www.mdpi.com/1422-0067/22/12/6382 |
| work_keys_str_mv | AT ekaterinaskovel toxicityandantioxidantactivityoffullerenolcsub6070subwithlownumberofoxygensubstituents AT arinagkicheeva toxicityandantioxidantactivityoffullerenolcsub6070subwithlownumberofoxygensubstituents AT nataliagvnukova toxicityandantioxidantactivityoffullerenolcsub6070subwithlownumberofoxygensubstituents AT grigorynchurilov toxicityandantioxidantactivityoffullerenolcsub6070subwithlownumberofoxygensubstituents AT evseiastepin toxicityandantioxidantactivityoffullerenolcsub6070subwithlownumberofoxygensubstituents AT nadezhdaskudryasheva toxicityandantioxidantactivityoffullerenolcsub6070subwithlownumberofoxygensubstituents |