Using bioinformatics technology to mine the expression of serum exosomal miRNA in patients with traumatic brain injury
IntroductionTraumatic brain injury (TBI) is considered the most common traumatic neurological disease, is associated with high mortality and long-term complications, and is a global public health issue. However, there has been little progress in serum markers for TBI research. Therefore, there is an...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2023.1145307/full |
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| author | Xintao Huang Xinjuan Xu Ce Wang Ce Wang Yi Wang Yi Wang Yajun Yang Yajun Yang Tianle Yao Rui Bai Xile Pei Feirong Bai Feirong Bai Panpan Li Panpan Li |
| author_facet | Xintao Huang Xinjuan Xu Ce Wang Ce Wang Yi Wang Yi Wang Yajun Yang Yajun Yang Tianle Yao Rui Bai Xile Pei Feirong Bai Feirong Bai Panpan Li Panpan Li |
| author_sort | Xintao Huang |
| collection | DOAJ |
| description | IntroductionTraumatic brain injury (TBI) is considered the most common traumatic neurological disease, is associated with high mortality and long-term complications, and is a global public health issue. However, there has been little progress in serum markers for TBI research. Therefore, there is an urgent need for biomarkers that can sufficiently function in TBI diagnosis and evaluation.MethodsExosomal microRNA (ExomiR), a stable circulating marker in the serum, has aroused widespread interest among researchers. To explore the level of serum ExomiR after TBI, we quantified ExomiR expression levels in serum exosomes extracted from patients with TBI using next-generation sequencing (NGS) and explored potential biomarkers using bioinformatics screening.ResultsCompared with the control group, there were 245 ExomiR (136 up-regulated and 109 down-regulated) in the serum of the TBI group that changed significantly. We observed serum ExomiRs expression profiles associated with neurovascular remodeling, the integrity of the blood-brain barrier, neuroinflammation, and a cascade of secondary injury, including eight up-regulated ExomiRs (ExomiR-124-3p, ExomiR-137-3p, ExomiR-9-3p, ExomiR-133a-5p, ExomiR-204-3p, ExomiR-519a-5p, ExomiR-4732-5p, and ExomiR-206) and 2 down-regulated ExomiR (ExomiR-21-3p and ExomiR-199a-5).DiscussionThe results revealed that serum ExomiRs might become a new research direction and breakthrough for the diagnosis and pathophysiological treatment of patients with TBI. |
| first_indexed | 2024-04-09T17:31:14Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-09T17:31:14Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-3e79b68a80fb469f998c3354aacc054b2023-04-18T05:18:40ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2023-04-011710.3389/fnins.2023.11453071145307Using bioinformatics technology to mine the expression of serum exosomal miRNA in patients with traumatic brain injuryXintao Huang0Xinjuan Xu1Ce Wang2Ce Wang3Yi Wang4Yi Wang5Yajun Yang6Yajun Yang7Tianle Yao8Rui Bai9Xile Pei10Feirong Bai11Feirong Bai12Panpan Li13Panpan Li14Department of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Neurosurgery, Shanxi Cardiovascular Hospital, Taiyuan, Shanxi, ChinaDepartment of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaThe First School of Clinical Medicine, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaThe First School of Clinical Medicine, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaThe First School of Clinical Medicine, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaThe First School of Clinical Medicine, Shanxi Medical University, Taiyuan, Shanxi, ChinaDepartment of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, ChinaThe First School of Clinical Medicine, Shanxi Medical University, Taiyuan, Shanxi, ChinaIntroductionTraumatic brain injury (TBI) is considered the most common traumatic neurological disease, is associated with high mortality and long-term complications, and is a global public health issue. However, there has been little progress in serum markers for TBI research. Therefore, there is an urgent need for biomarkers that can sufficiently function in TBI diagnosis and evaluation.MethodsExosomal microRNA (ExomiR), a stable circulating marker in the serum, has aroused widespread interest among researchers. To explore the level of serum ExomiR after TBI, we quantified ExomiR expression levels in serum exosomes extracted from patients with TBI using next-generation sequencing (NGS) and explored potential biomarkers using bioinformatics screening.ResultsCompared with the control group, there were 245 ExomiR (136 up-regulated and 109 down-regulated) in the serum of the TBI group that changed significantly. We observed serum ExomiRs expression profiles associated with neurovascular remodeling, the integrity of the blood-brain barrier, neuroinflammation, and a cascade of secondary injury, including eight up-regulated ExomiRs (ExomiR-124-3p, ExomiR-137-3p, ExomiR-9-3p, ExomiR-133a-5p, ExomiR-204-3p, ExomiR-519a-5p, ExomiR-4732-5p, and ExomiR-206) and 2 down-regulated ExomiR (ExomiR-21-3p and ExomiR-199a-5).DiscussionThe results revealed that serum ExomiRs might become a new research direction and breakthrough for the diagnosis and pathophysiological treatment of patients with TBI.https://www.frontiersin.org/articles/10.3389/fnins.2023.1145307/fulltraumatic brain injuryexosomemicroRNA (miR)next-generation sequencing (NGS)bioinformatics analysis |
| spellingShingle | Xintao Huang Xinjuan Xu Ce Wang Ce Wang Yi Wang Yi Wang Yajun Yang Yajun Yang Tianle Yao Rui Bai Xile Pei Feirong Bai Feirong Bai Panpan Li Panpan Li Using bioinformatics technology to mine the expression of serum exosomal miRNA in patients with traumatic brain injury Frontiers in Neuroscience traumatic brain injury exosome microRNA (miR) next-generation sequencing (NGS) bioinformatics analysis |
| title | Using bioinformatics technology to mine the expression of serum exosomal miRNA in patients with traumatic brain injury |
| title_full | Using bioinformatics technology to mine the expression of serum exosomal miRNA in patients with traumatic brain injury |
| title_fullStr | Using bioinformatics technology to mine the expression of serum exosomal miRNA in patients with traumatic brain injury |
| title_full_unstemmed | Using bioinformatics technology to mine the expression of serum exosomal miRNA in patients with traumatic brain injury |
| title_short | Using bioinformatics technology to mine the expression of serum exosomal miRNA in patients with traumatic brain injury |
| title_sort | using bioinformatics technology to mine the expression of serum exosomal mirna in patients with traumatic brain injury |
| topic | traumatic brain injury exosome microRNA (miR) next-generation sequencing (NGS) bioinformatics analysis |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2023.1145307/full |
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