GLP-1/GLP-1R Signaling in Regulation of Adipocyte Differentiation and Lipogenesis
Background/Aims: The aim of this study was to determine the direct role of liraglutide (LG) in adipogenesis and lipid metabolism. Methods: Lipid accumulation was evaluated by oil red O staining, quantitative real-time PCR (qPCR) was performed to determine glucagon-like peptide 1 receptor (GLP-1R), f...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2017-06-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/478872 |
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| author | Jicui Chen Huichen Zhao Xiaoli Ma Yuchao Zhang Sumei Lu Yangang Wang Chen Zong Dandan Qin Yuanmei Wang Yingfeng Yingfeng Yang Xiangdong Wang Yuantao Liu |
| author_facet | Jicui Chen Huichen Zhao Xiaoli Ma Yuchao Zhang Sumei Lu Yangang Wang Chen Zong Dandan Qin Yuanmei Wang Yingfeng Yingfeng Yang Xiangdong Wang Yuantao Liu |
| author_sort | Jicui Chen |
| collection | DOAJ |
| description | Background/Aims: The aim of this study was to determine the direct role of liraglutide (LG) in adipogenesis and lipid metabolism. Methods: Lipid accumulation was evaluated by oil red O staining, quantitative real-time PCR (qPCR) was performed to determine glucagon-like peptide 1 receptor (GLP-1R), fatty acid synthase (FASN) and adipose triglyceride lipase (ATGL) expression in 3T3-L1 preadipocytes, differentiated adipocytes and in adipose tissues from mice. The effects of LG on 3T3-L1 adipogenesis and lipid metabolism were analyzed with qPCR, Western Blotting, oil red O staining, immunohistochemistry (IHC) and immunofluorescence (IF). All measurements were performed at least three times. Results: LG increased the expression of differentiation marker genes and lipid accumulation during preadipocyte differentiation. In differentiated adipocytes, LG decreased FASN expression, and simultaneously led to CREB phosphorylation and ERK1/2 activation which were abolished by a GLP-1R antagonist, exendin (9-39). LG induced-FASN down-regulation was partially reversed by PKA and ERK1/2 inhibitors. Consistent with above in vitro findings, LG treatment significantly reduced FASN expression in visceral adipose tissues of ob/ob mice, and reduced body weight gain. Conclusion: LG promotes preadipocytes differentiation, and inhibits FASN expression in adipocytes. LG induced down-regulation of FASN is at least partially mediated by PKA and MAPK signaling pathways. |
| first_indexed | 2024-12-21T18:32:22Z |
| format | Article |
| id | doaj.art-3e7f343fdf564be3bff25449c0392eff |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-21T18:32:22Z |
| publishDate | 2017-06-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-3e7f343fdf564be3bff25449c0392eff2022-12-21T18:54:14ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-06-014231165117610.1159/000478872478872GLP-1/GLP-1R Signaling in Regulation of Adipocyte Differentiation and LipogenesisJicui ChenHuichen ZhaoXiaoli MaYuchao ZhangSumei LuYangang WangChen ZongDandan QinYuanmei WangYingfeng Yingfeng YangXiangdong WangYuantao LiuBackground/Aims: The aim of this study was to determine the direct role of liraglutide (LG) in adipogenesis and lipid metabolism. Methods: Lipid accumulation was evaluated by oil red O staining, quantitative real-time PCR (qPCR) was performed to determine glucagon-like peptide 1 receptor (GLP-1R), fatty acid synthase (FASN) and adipose triglyceride lipase (ATGL) expression in 3T3-L1 preadipocytes, differentiated adipocytes and in adipose tissues from mice. The effects of LG on 3T3-L1 adipogenesis and lipid metabolism were analyzed with qPCR, Western Blotting, oil red O staining, immunohistochemistry (IHC) and immunofluorescence (IF). All measurements were performed at least three times. Results: LG increased the expression of differentiation marker genes and lipid accumulation during preadipocyte differentiation. In differentiated adipocytes, LG decreased FASN expression, and simultaneously led to CREB phosphorylation and ERK1/2 activation which were abolished by a GLP-1R antagonist, exendin (9-39). LG induced-FASN down-regulation was partially reversed by PKA and ERK1/2 inhibitors. Consistent with above in vitro findings, LG treatment significantly reduced FASN expression in visceral adipose tissues of ob/ob mice, and reduced body weight gain. Conclusion: LG promotes preadipocytes differentiation, and inhibits FASN expression in adipocytes. LG induced down-regulation of FASN is at least partially mediated by PKA and MAPK signaling pathways.http://www.karger.com/Article/FullText/478872Liraglutide3T3-L1 preadipocytesAdipogenesisFASN |
| spellingShingle | Jicui Chen Huichen Zhao Xiaoli Ma Yuchao Zhang Sumei Lu Yangang Wang Chen Zong Dandan Qin Yuanmei Wang Yingfeng Yingfeng Yang Xiangdong Wang Yuantao Liu GLP-1/GLP-1R Signaling in Regulation of Adipocyte Differentiation and Lipogenesis Cellular Physiology and Biochemistry Liraglutide 3T3-L1 preadipocytes Adipogenesis FASN |
| title | GLP-1/GLP-1R Signaling in Regulation of Adipocyte Differentiation and Lipogenesis |
| title_full | GLP-1/GLP-1R Signaling in Regulation of Adipocyte Differentiation and Lipogenesis |
| title_fullStr | GLP-1/GLP-1R Signaling in Regulation of Adipocyte Differentiation and Lipogenesis |
| title_full_unstemmed | GLP-1/GLP-1R Signaling in Regulation of Adipocyte Differentiation and Lipogenesis |
| title_short | GLP-1/GLP-1R Signaling in Regulation of Adipocyte Differentiation and Lipogenesis |
| title_sort | glp 1 glp 1r signaling in regulation of adipocyte differentiation and lipogenesis |
| topic | Liraglutide 3T3-L1 preadipocytes Adipogenesis FASN |
| url | http://www.karger.com/Article/FullText/478872 |
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