Role of miRNAs in Sigmoid Colon Cancer: A Search for Potential Biomarkers
The aberrant expression of microRNAs in known to play a crucial role in carcinogenesis. Here, we evaluated the miRNA expression profile of sigmoid colon cancer (SCC) compared to adjacent-to-tumor (ADJ) and sigmoid colon healthy (SCH) tissues obtained from colon biopsy extracted from Brazilian patien...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/12/11/3311 |
| _version_ | 1797548388599726080 |
|---|---|
| author | Diego Marques Layse Raynara Ferreira-Costa Lorenna Larissa Ferreira-Costa Ana Beatriz Bezerra-Oliveira Romualdo da Silva Correa Carlos Cesar de Oliveira Ramos Tatiana Vinasco-Sandoval Katia de Paiva Lopes Ricardo Assunção Vialle Amanda Ferreira Vidal Vivian Nogueira Silbiger Ândrea Ribeiro-dos-Santos |
| author_facet | Diego Marques Layse Raynara Ferreira-Costa Lorenna Larissa Ferreira-Costa Ana Beatriz Bezerra-Oliveira Romualdo da Silva Correa Carlos Cesar de Oliveira Ramos Tatiana Vinasco-Sandoval Katia de Paiva Lopes Ricardo Assunção Vialle Amanda Ferreira Vidal Vivian Nogueira Silbiger Ândrea Ribeiro-dos-Santos |
| author_sort | Diego Marques |
| collection | DOAJ |
| description | The aberrant expression of microRNAs in known to play a crucial role in carcinogenesis. Here, we evaluated the miRNA expression profile of sigmoid colon cancer (SCC) compared to adjacent-to-tumor (ADJ) and sigmoid colon healthy (SCH) tissues obtained from colon biopsy extracted from Brazilian patients. Comparisons were performed between each group separately, considering as significant <i>p</i>-values < 0.05 and |Log<sub>2</sub>(Fold-Change)| > 2. We found 20 differentially expressed miRNAs (DEmiRNAs) in all comparisons, two of which were shared between SCC vs. ADJ and SCC vs. SCH. We used miRTarBase, and miRTargetLink to identify target-genes of the differentially expressed miRNAs, and DAVID and REACTOME databases for gene enrichment analysis. We also used TCGA and GTEx databases to build miRNA-gene regulatory networks and check for the reproducibility in our results. As findings, in addition to previously known miRNAs associated with colorectal cancer, we identified three potential novel biomarkers. We showed that the three types of colon tissue could be clearly distinguished using a panel composed by the 20 DEmiRNAs. Additionally, we found enriched pathways related to the carcinogenic process in which miRNA could be involved, indicating that adjacent-to-tumor tissues may be already altered and cannot be considered as healthy tissues. Overall, we expect that these findings may help in the search for biomarkers to prevent cancer progression or, at least, allow its early detection, however, more studies are needed to confirm our results. |
| first_indexed | 2024-03-10T14:58:40Z |
| format | Article |
| id | doaj.art-3e7f6639f43448e29d8f0b3d48ebe200 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T14:58:40Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-3e7f6639f43448e29d8f0b3d48ebe2002023-11-20T20:21:38ZengMDPI AGCancers2072-66942020-11-011211331110.3390/cancers12113311Role of miRNAs in Sigmoid Colon Cancer: A Search for Potential BiomarkersDiego Marques0Layse Raynara Ferreira-Costa1Lorenna Larissa Ferreira-Costa2Ana Beatriz Bezerra-Oliveira3Romualdo da Silva Correa4Carlos Cesar de Oliveira Ramos5Tatiana Vinasco-Sandoval6Katia de Paiva Lopes7Ricardo Assunção Vialle8Amanda Ferreira Vidal9Vivian Nogueira Silbiger10Ândrea Ribeiro-dos-Santos11Laboratório de Genética Humana e Médica, Universidade Federal do Pará, Av. Augusto Corrêa, 01, Guamá, Belém 66.075-110, BrazilLaboratório de Bioanálise e Biotecnologia Molecular, Universidade Federal do Rio Grande do Norte, Av. Nilo Peçanha, 620, Petrópolis, Natal 59012-300, BrazilLaboratório de Bioanálise e Biotecnologia Molecular, Universidade Federal do Rio Grande do Norte, Av. Nilo Peçanha, 620, Petrópolis, Natal 59012-300, BrazilLaboratório de Bioanálise e Biotecnologia Molecular, Universidade Federal do Rio Grande do Norte, Av. Nilo Peçanha, 620, Petrópolis, Natal 59012-300, BrazilDepartamento de Cirurgia Oncológica, Liga Norte Riograndense Contra o Câncer, R. Mário Negócio, 2267, Quintas, Natal 59040-000, BrazilLaboratório de Patologia e Citopatologia, Liga Norte Riograndense Contra o Câncer, R. Mário Negócio, 2267, Quintas, Natal 59040-000, BrazilLaboratório de Genética Humana e Médica, Universidade Federal do Pará, Av. Augusto Corrêa, 01, Guamá, Belém 66.075-110, BrazilLaboratório de Genética Humana e Médica, Universidade Federal do Pará, Av. Augusto Corrêa, 01, Guamá, Belém 66.075-110, BrazilLaboratório de Genética Humana e Médica, Universidade Federal do Pará, Av. Augusto Corrêa, 01, Guamá, Belém 66.075-110, BrazilLaboratório de Genética Humana e Médica, Universidade Federal do Pará, Av. Augusto Corrêa, 01, Guamá, Belém 66.075-110, BrazilLaboratório de Bioanálise e Biotecnologia Molecular, Universidade Federal do Rio Grande do Norte, Av. Nilo Peçanha, 620, Petrópolis, Natal 59012-300, BrazilLaboratório de Genética Humana e Médica, Universidade Federal do Pará, Av. Augusto Corrêa, 01, Guamá, Belém 66.075-110, BrazilThe aberrant expression of microRNAs in known to play a crucial role in carcinogenesis. Here, we evaluated the miRNA expression profile of sigmoid colon cancer (SCC) compared to adjacent-to-tumor (ADJ) and sigmoid colon healthy (SCH) tissues obtained from colon biopsy extracted from Brazilian patients. Comparisons were performed between each group separately, considering as significant <i>p</i>-values < 0.05 and |Log<sub>2</sub>(Fold-Change)| > 2. We found 20 differentially expressed miRNAs (DEmiRNAs) in all comparisons, two of which were shared between SCC vs. ADJ and SCC vs. SCH. We used miRTarBase, and miRTargetLink to identify target-genes of the differentially expressed miRNAs, and DAVID and REACTOME databases for gene enrichment analysis. We also used TCGA and GTEx databases to build miRNA-gene regulatory networks and check for the reproducibility in our results. As findings, in addition to previously known miRNAs associated with colorectal cancer, we identified three potential novel biomarkers. We showed that the three types of colon tissue could be clearly distinguished using a panel composed by the 20 DEmiRNAs. Additionally, we found enriched pathways related to the carcinogenic process in which miRNA could be involved, indicating that adjacent-to-tumor tissues may be already altered and cannot be considered as healthy tissues. Overall, we expect that these findings may help in the search for biomarkers to prevent cancer progression or, at least, allow its early detection, however, more studies are needed to confirm our results.https://www.mdpi.com/2072-6694/12/11/3311biomarkersfield-effectcolorectal cancermiRNome |
| spellingShingle | Diego Marques Layse Raynara Ferreira-Costa Lorenna Larissa Ferreira-Costa Ana Beatriz Bezerra-Oliveira Romualdo da Silva Correa Carlos Cesar de Oliveira Ramos Tatiana Vinasco-Sandoval Katia de Paiva Lopes Ricardo Assunção Vialle Amanda Ferreira Vidal Vivian Nogueira Silbiger Ândrea Ribeiro-dos-Santos Role of miRNAs in Sigmoid Colon Cancer: A Search for Potential Biomarkers Cancers biomarkers field-effect colorectal cancer miRNome |
| title | Role of miRNAs in Sigmoid Colon Cancer: A Search for Potential Biomarkers |
| title_full | Role of miRNAs in Sigmoid Colon Cancer: A Search for Potential Biomarkers |
| title_fullStr | Role of miRNAs in Sigmoid Colon Cancer: A Search for Potential Biomarkers |
| title_full_unstemmed | Role of miRNAs in Sigmoid Colon Cancer: A Search for Potential Biomarkers |
| title_short | Role of miRNAs in Sigmoid Colon Cancer: A Search for Potential Biomarkers |
| title_sort | role of mirnas in sigmoid colon cancer a search for potential biomarkers |
| topic | biomarkers field-effect colorectal cancer miRNome |
| url | https://www.mdpi.com/2072-6694/12/11/3311 |
| work_keys_str_mv | AT diegomarques roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT layseraynaraferreiracosta roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT lorennalarissaferreiracosta roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT anabeatrizbezerraoliveira roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT romualdodasilvacorrea roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT carloscesardeoliveiraramos roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT tatianavinascosandoval roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT katiadepaivalopes roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT ricardoassuncaovialle roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT amandaferreiravidal roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT viviannogueirasilbiger roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers AT andrearibeirodossantos roleofmirnasinsigmoidcoloncancerasearchforpotentialbiomarkers |