Investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virus
ObjectiveHuman papillomaviruses (HPVs) are among the agents responsible for infection and cancer of the skin and mucous membranes in the human body. The aim of this study was to investigate the frequency and type distribution of HPVs in married female patients with gynecological complaints, who had...
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| Format: | Article |
| Language: | English |
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Korean Society of Obstetrics and Gynecology
2019-07-01
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| Series: | Obstetrics & Gynecology Science |
| Subjects: | |
| Online Access: | http://ogscience.org/upload/pdf/ogs-62-264.pdf |
| _version_ | 1818577000646836224 |
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| author | Ayse Erdem Yayla Berrin Goktug Kadioglu Ayse Aydin Osman Aktas |
| author_facet | Ayse Erdem Yayla Berrin Goktug Kadioglu Ayse Aydin Osman Aktas |
| author_sort | Ayse Erdem Yayla |
| collection | DOAJ |
| description | ObjectiveHuman papillomaviruses (HPVs) are among the agents responsible for infection and cancer of the skin and mucous membranes in the human body. The aim of this study was to investigate the frequency and type distribution of HPVs in married female patients with gynecological complaints, who had visited the Maternity Hospital in Erzurum, Turkey.MethodsIn this study, 263 cervical swab samples were taken from married women using the Pap smear method and were investigated for positive reactivity against HPV. The L1 gene region of HPV was investigated using molecular methods. For this purpose, polymerase chain reaction (PCR) assays and sequence analysis of positive samples were performed. Phylogenetic analyses were performed using a bioinformatics approach after sequencing.ResultsHPV-DNA was detected in 17 (6.5%) samples. Highest positive reactivity to HPV-DNA was found in the 35–44 age group at 9.2%. Fourteen out of seventeen positive samples were included in the phylogenetic analysis. All isolates clustered in the Alphapapillomavirus genus. Six samples were found to be HPV 70 positive, four were HPV 16 positive, and the rest were HPV 54, 72, 81, and 114 positive. When genotyping data were evaluated according to the risk group, we found that 28.6% of the 14 samples were found to be high risk-HPV, and 71.4% were low risk-HPV.ConclusionsAs per our knowledge, this is the first report on the phylogenetic analysis of HPV genotypes isolated from women in Turkey. The prevalence of low- and-high risk HPV was determined in married women in Erzurum, and these results contribute to the epidemiological data on the distribution of HPV types for this region. |
| first_indexed | 2024-12-16T06:22:57Z |
| format | Article |
| id | doaj.art-3e84364e56474e0ab4d23922b4e4706b |
| institution | Directory Open Access Journal |
| issn | 2287-8572 2287-8580 |
| language | English |
| last_indexed | 2024-12-16T06:22:57Z |
| publishDate | 2019-07-01 |
| publisher | Korean Society of Obstetrics and Gynecology |
| record_format | Article |
| series | Obstetrics & Gynecology Science |
| spelling | doaj.art-3e84364e56474e0ab4d23922b4e4706b2022-12-21T22:41:05ZengKorean Society of Obstetrics and GynecologyObstetrics & Gynecology Science2287-85722287-85802019-07-0162426427210.5468/ogs.2019.62.4.264583Investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virusAyse Erdem Yayla0Berrin Goktug Kadioglu1Ayse Aydin2Osman Aktas3Department of Medical Microbiology, School of Medicine, Atatürk University, Erzurum, .TurkeyDepartment of Gynecology and Obstetrics, Health Sciences University, Erzurum Regional Training and Research Hospital, Erzurum, .TurkeyDepartment of Birth, Women's Health and Diseases Nursing, Faculty of Nursing, Atatürk University, Erzurum, .TurkeyDepartment of Medical Microbiology, School of Medicine, Atatürk University, Erzurum, .TurkeyObjectiveHuman papillomaviruses (HPVs) are among the agents responsible for infection and cancer of the skin and mucous membranes in the human body. The aim of this study was to investigate the frequency and type distribution of HPVs in married female patients with gynecological complaints, who had visited the Maternity Hospital in Erzurum, Turkey.MethodsIn this study, 263 cervical swab samples were taken from married women using the Pap smear method and were investigated for positive reactivity against HPV. The L1 gene region of HPV was investigated using molecular methods. For this purpose, polymerase chain reaction (PCR) assays and sequence analysis of positive samples were performed. Phylogenetic analyses were performed using a bioinformatics approach after sequencing.ResultsHPV-DNA was detected in 17 (6.5%) samples. Highest positive reactivity to HPV-DNA was found in the 35–44 age group at 9.2%. Fourteen out of seventeen positive samples were included in the phylogenetic analysis. All isolates clustered in the Alphapapillomavirus genus. Six samples were found to be HPV 70 positive, four were HPV 16 positive, and the rest were HPV 54, 72, 81, and 114 positive. When genotyping data were evaluated according to the risk group, we found that 28.6% of the 14 samples were found to be high risk-HPV, and 71.4% were low risk-HPV.ConclusionsAs per our knowledge, this is the first report on the phylogenetic analysis of HPV genotypes isolated from women in Turkey. The prevalence of low- and-high risk HPV was determined in married women in Erzurum, and these results contribute to the epidemiological data on the distribution of HPV types for this region.http://ogscience.org/upload/pdf/ogs-62-264.pdfpapillomavirus infectionscervical smearspolymerase chain reactionturkey |
| spellingShingle | Ayse Erdem Yayla Berrin Goktug Kadioglu Ayse Aydin Osman Aktas Investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virus Obstetrics & Gynecology Science papillomavirus infections cervical smears polymerase chain reaction turkey |
| title | Investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virus |
| title_full | Investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virus |
| title_fullStr | Investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virus |
| title_full_unstemmed | Investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virus |
| title_short | Investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virus |
| title_sort | investigation of human papillomavirus prevalence in married women and molecular characterization and phylogenetic analysis of the virus |
| topic | papillomavirus infections cervical smears polymerase chain reaction turkey |
| url | http://ogscience.org/upload/pdf/ogs-62-264.pdf |
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