Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?
<b>Purpose:</b> To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b> Materials and Methods:</b> Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA),...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2010-01-01
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| Series: | Indian Journal of Ophthalmology |
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| Online Access: | http://www.ijo.in/article.asp?issn=0301-4738;year=2010;volume=58;issue=4;spage=281;epage=286;aulast=Das |
| _version_ | 1818145542528565248 |
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| author | Das Sujata Sharma Savitri Kar Sarita Sahu Srikant Samal Bikash Mallick Aparajita |
| author_facet | Das Sujata Sharma Savitri Kar Sarita Sahu Srikant Samal Bikash Mallick Aparajita |
| author_sort | Das Sujata |
| collection | DOAJ |
| description | <b>Purpose:</b> To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b> Materials and Methods:</b> Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i>Escherichia coli </i>overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b> Results:</b> Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i>P</i> = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i>in vitro</i> experiment although the characteristic spores and color were seen earlier on SDA. <b> Conclusion:</b> Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus. |
| first_indexed | 2024-12-11T12:05:07Z |
| format | Article |
| id | doaj.art-3e84f5a3f2324662b3c0cc52fb8350e1 |
| institution | Directory Open Access Journal |
| issn | 0301-4738 1998-3689 |
| language | English |
| last_indexed | 2024-12-11T12:05:07Z |
| publishDate | 2010-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Ophthalmology |
| spelling | doaj.art-3e84f5a3f2324662b3c0cc52fb8350e12022-12-22T01:07:58ZengWolters Kluwer Medknow PublicationsIndian Journal of Ophthalmology0301-47381998-36892010-01-01584281286Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?Das SujataSharma SavitriKar SaritaSahu SrikantSamal BikashMallick Aparajita<b>Purpose:</b> To determine whether the inclusion of Sabouraud dextrose agar (SDA) is essential in the diagnosis of fungal keratitis. <b> Materials and Methods:</b> Corneal scrapings of 141 patients with microbial keratitis were smeared and cultured. Sheep blood agar (BA), chocolate agar (CA), SDA, non-nutrient agar (NNA) with <i>Escherichia coli </i>overlay, and brain heart infusion broth (BHI) were evaluated for time taken for growth and cost. The media were also evaluated experimentally for rate of growth and time taken for identification. <b> Results:</b> Twenty-six of 39 patients positive for fungus in corneal scrapings by microscopy were culture-positive. Fungus grew on BA in 22/39, on CA in 18/39, on SDA in 17/39, on NNA in 17/39, and on BHI in 13/39 cases. Growth on SDA was higher in ulcers with larger infiltrate (6/18 versus 9/13, <i>P</i> = 0.04). Estimated saving with inclusion of only BA/CA was Rs. 600 per patient. Performance of all media was similar in <i>in vitro</i> experiment although the characteristic spores and color were seen earlier on SDA. <b> Conclusion:</b> Fungal keratitis can be reliably confirmed on BA or CA, which support growth of both bacteria and fungus.http://www.ijo.in/article.asp?issn=0301-4738;year=2010;volume=58;issue=4;spage=281;epage=286;aulast=DasBlood agarchocolate agardiagnosisfungal keratitisSabouraud dextrose agar |
| spellingShingle | Das Sujata Sharma Savitri Kar Sarita Sahu Srikant Samal Bikash Mallick Aparajita Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? Indian Journal of Ophthalmology Blood agar chocolate agar diagnosis fungal keratitis Sabouraud dextrose agar |
| title | Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? |
| title_full | Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? |
| title_fullStr | Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? |
| title_full_unstemmed | Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? |
| title_short | Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis? |
| title_sort | is inclusion of sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis |
| topic | Blood agar chocolate agar diagnosis fungal keratitis Sabouraud dextrose agar |
| url | http://www.ijo.in/article.asp?issn=0301-4738;year=2010;volume=58;issue=4;spage=281;epage=286;aulast=Das |
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