Inhibitory Effect of <i>Ulmus davidiana</i> and <i>Cornus officinalis</i> Extracts on Osteoporotic Bone Loss In Vitro and In Vivo
<i>Background and Objectives:</i> Traditional herbal medicines are becoming more popular as a complementary medication as they have the advantages of being mostly harmless and safe, causing fewer side-effects than conventional medications. Here, we demonstrate the inhibitory effects of t...
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MDPI AG
2022-03-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/58/4/466 |
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| author | Jeonghyun Kim Chang-Gun Lee Seung-Hee Yun Seokjin Hwang Hyoju Jeon Eunkuk Park Seon-Yong Jeong |
| author_facet | Jeonghyun Kim Chang-Gun Lee Seung-Hee Yun Seokjin Hwang Hyoju Jeon Eunkuk Park Seon-Yong Jeong |
| author_sort | Jeonghyun Kim |
| collection | DOAJ |
| description | <i>Background and Objectives:</i> Traditional herbal medicines are becoming more popular as a complementary medication as they have the advantages of being mostly harmless and safe, causing fewer side-effects than conventional medications. Here, we demonstrate the inhibitory effects of the combination of <i>Ulmus davidiana</i> (UD) and <i>Cornus officinalis</i> (CO) extracts on osteoporotic bone loss. <i>Materials and Methods</i>: This study presented osteogenic effects in primary cultured osteoblasts, pre-osteoblastic MC3T3-E1 cell lines, and osteoclastogenic effects in osteoclasts derived from bone marrow monocytes, and finally, protective effects on bone loss in an ovariectomy (OVX)-induced osteoporotic animal model. <i>Results</i>: A significant increase in alkaline phosphatase (ALP) activity was observed following treatment with UD and CO mixtures (8:2, 7:3, and 5:5 ratios) and individual UD and CO extracts, with the highest ALP activity being detected for the treatment with UD and CO extracts at a 5:5 ratio. An optimal ratio of UD and CO (UC) extract promoted osteoblast differentiation in both pre-osteoblastic cells and primary osteoblasts by increasing osteoblastic markers such as <i>Alpl</i>, <i>Runx2</i>, and <i>Bglap</i>. However, treatment with the UC extract inhibited osteoclast differentiation with a decreased expression of osteoclastogenesis-related genes, including <i>Ctsk</i>, <i>Acp5</i>, <i>Mmp9</i>, and <i>Nfatc1</i>. In addition, UC treatment prevented osteoporotic bone loss in OVX mice and improved impaired skeletal structure parameters. <i>Conclusions</i>: This study suggests that combined UD and CO extracts may be a beneficial traditional medicine for the prevention of postmenopausal osteoporosis. |
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| language | English |
| last_indexed | 2024-03-09T04:25:39Z |
| publishDate | 2022-03-01 |
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| series | Medicina |
| spelling | doaj.art-3e874b2181e843189c0672bd469984382023-12-03T13:40:57ZengMDPI AGMedicina1010-660X1648-91442022-03-0158446610.3390/medicina58040466Inhibitory Effect of <i>Ulmus davidiana</i> and <i>Cornus officinalis</i> Extracts on Osteoporotic Bone Loss In Vitro and In VivoJeonghyun Kim0Chang-Gun Lee1Seung-Hee Yun2Seokjin Hwang3Hyoju Jeon4Eunkuk Park5Seon-Yong Jeong6Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea<i>Background and Objectives:</i> Traditional herbal medicines are becoming more popular as a complementary medication as they have the advantages of being mostly harmless and safe, causing fewer side-effects than conventional medications. Here, we demonstrate the inhibitory effects of the combination of <i>Ulmus davidiana</i> (UD) and <i>Cornus officinalis</i> (CO) extracts on osteoporotic bone loss. <i>Materials and Methods</i>: This study presented osteogenic effects in primary cultured osteoblasts, pre-osteoblastic MC3T3-E1 cell lines, and osteoclastogenic effects in osteoclasts derived from bone marrow monocytes, and finally, protective effects on bone loss in an ovariectomy (OVX)-induced osteoporotic animal model. <i>Results</i>: A significant increase in alkaline phosphatase (ALP) activity was observed following treatment with UD and CO mixtures (8:2, 7:3, and 5:5 ratios) and individual UD and CO extracts, with the highest ALP activity being detected for the treatment with UD and CO extracts at a 5:5 ratio. An optimal ratio of UD and CO (UC) extract promoted osteoblast differentiation in both pre-osteoblastic cells and primary osteoblasts by increasing osteoblastic markers such as <i>Alpl</i>, <i>Runx2</i>, and <i>Bglap</i>. However, treatment with the UC extract inhibited osteoclast differentiation with a decreased expression of osteoclastogenesis-related genes, including <i>Ctsk</i>, <i>Acp5</i>, <i>Mmp9</i>, and <i>Nfatc1</i>. In addition, UC treatment prevented osteoporotic bone loss in OVX mice and improved impaired skeletal structure parameters. <i>Conclusions</i>: This study suggests that combined UD and CO extracts may be a beneficial traditional medicine for the prevention of postmenopausal osteoporosis.https://www.mdpi.com/1648-9144/58/4/466<i>Ulmus davidiana</i><i>Cornus officinalis</i>primary osteoblastsprimary osteoclastsovariectomized miceherbal medicine |
| spellingShingle | Jeonghyun Kim Chang-Gun Lee Seung-Hee Yun Seokjin Hwang Hyoju Jeon Eunkuk Park Seon-Yong Jeong Inhibitory Effect of <i>Ulmus davidiana</i> and <i>Cornus officinalis</i> Extracts on Osteoporotic Bone Loss In Vitro and In Vivo Medicina <i>Ulmus davidiana</i> <i>Cornus officinalis</i> primary osteoblasts primary osteoclasts ovariectomized mice herbal medicine |
| title | Inhibitory Effect of <i>Ulmus davidiana</i> and <i>Cornus officinalis</i> Extracts on Osteoporotic Bone Loss In Vitro and In Vivo |
| title_full | Inhibitory Effect of <i>Ulmus davidiana</i> and <i>Cornus officinalis</i> Extracts on Osteoporotic Bone Loss In Vitro and In Vivo |
| title_fullStr | Inhibitory Effect of <i>Ulmus davidiana</i> and <i>Cornus officinalis</i> Extracts on Osteoporotic Bone Loss In Vitro and In Vivo |
| title_full_unstemmed | Inhibitory Effect of <i>Ulmus davidiana</i> and <i>Cornus officinalis</i> Extracts on Osteoporotic Bone Loss In Vitro and In Vivo |
| title_short | Inhibitory Effect of <i>Ulmus davidiana</i> and <i>Cornus officinalis</i> Extracts on Osteoporotic Bone Loss In Vitro and In Vivo |
| title_sort | inhibitory effect of i ulmus davidiana i and i cornus officinalis i extracts on osteoporotic bone loss in vitro and in vivo |
| topic | <i>Ulmus davidiana</i> <i>Cornus officinalis</i> primary osteoblasts primary osteoclasts ovariectomized mice herbal medicine |
| url | https://www.mdpi.com/1648-9144/58/4/466 |
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