A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod.
We have conducted a protein interaction study of components within a specific sub-compartment of a eukaryotic flagellum. The trypanosome flagellum contains a para-crystalline extra-axonemal structure termed the paraflagellar rod (PFR) with around forty identified components. We have used a Gateway c...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-11-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2766642?pdf=render |
| _version_ | 1798043278370668544 |
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| author | Sylvain Lacomble Neil Portman Keith Gull |
| author_facet | Sylvain Lacomble Neil Portman Keith Gull |
| author_sort | Sylvain Lacomble |
| collection | DOAJ |
| description | We have conducted a protein interaction study of components within a specific sub-compartment of a eukaryotic flagellum. The trypanosome flagellum contains a para-crystalline extra-axonemal structure termed the paraflagellar rod (PFR) with around forty identified components. We have used a Gateway cloning approach coupled with yeast two-hybrid, RNAi and 2D DiGE to define a protein-protein interaction network taking place in this structure. We define two clusters of interactions; the first being characterised by two proteins with a shared domain which is not sufficient for maintaining the interaction. The other cohort is populated by eight proteins, a number of which possess a PFR domain and sub-populations of this network exhibit dependency relationships. Finally, we provide clues as to the structural organisation of the PFR at the molecular level. This multi-strand approach shows that protein interactome data can be generated for insoluble protein complexes. |
| first_indexed | 2024-04-11T22:47:05Z |
| format | Article |
| id | doaj.art-3e88c5df4697477da7d8b5b85dea0003 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T22:47:05Z |
| publishDate | 2009-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3e88c5df4697477da7d8b5b85dea00032022-12-22T03:58:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-11-01411e768510.1371/journal.pone.0007685A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod.Sylvain LacombleNeil PortmanKeith GullWe have conducted a protein interaction study of components within a specific sub-compartment of a eukaryotic flagellum. The trypanosome flagellum contains a para-crystalline extra-axonemal structure termed the paraflagellar rod (PFR) with around forty identified components. We have used a Gateway cloning approach coupled with yeast two-hybrid, RNAi and 2D DiGE to define a protein-protein interaction network taking place in this structure. We define two clusters of interactions; the first being characterised by two proteins with a shared domain which is not sufficient for maintaining the interaction. The other cohort is populated by eight proteins, a number of which possess a PFR domain and sub-populations of this network exhibit dependency relationships. Finally, we provide clues as to the structural organisation of the PFR at the molecular level. This multi-strand approach shows that protein interactome data can be generated for insoluble protein complexes.http://europepmc.org/articles/PMC2766642?pdf=render |
| spellingShingle | Sylvain Lacomble Neil Portman Keith Gull A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod. PLoS ONE |
| title | A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod. |
| title_full | A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod. |
| title_fullStr | A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod. |
| title_full_unstemmed | A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod. |
| title_short | A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod. |
| title_sort | protein protein interaction map of the trypanosoma brucei paraflagellar rod |
| url | http://europepmc.org/articles/PMC2766642?pdf=render |
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