CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant
Abstract Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now, which is time...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-08-01
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| Series: | Nano-Micro Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s40820-022-00888-4 |
| _version_ | 1818505500030926848 |
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| author | Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao Yuxuan Tang Yuxuan Yuan Luxiao Chai Taojian Fan Jiangtian Yu Xiaopeng Ma Omar A. Al-Hartomy S. Wageh Abdullah G. Al-Sehemi Zhiguang Luo Yaqing He Jingfeng Li Zhongjian Xie Han Zhang |
| author_facet | Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao Yuxuan Tang Yuxuan Yuan Luxiao Chai Taojian Fan Jiangtian Yu Xiaopeng Ma Omar A. Al-Hartomy S. Wageh Abdullah G. Al-Sehemi Zhiguang Luo Yaqing He Jingfeng Li Zhongjian Xie Han Zhang |
| author_sort | Chenshuo Wu |
| collection | DOAJ |
| description | Abstract Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now, which is time-consuming and requires expensive instrumentation, and the confirmation of variants relies on further sequencing techniques. Herein, we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid, highly sensitivity and specificity for the detection of SARS-CoV-2 variant. To enhance the sensing capability, gold electrodes are uniformly decorated with electro-deposited gold nanoparticles. Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model, our biosensor exhibits excellent analytical detection limit (50 fM) and high linearity (R 2 = 0.987) over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays. The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system. Furthermore, based on the wireless micro-electrochemical platform, the proposed biosensor reveals promising application ability in point-of-care testing. |
| first_indexed | 2024-12-10T21:51:37Z |
| format | Article |
| id | doaj.art-3e8c6e0daf524eafb7c7a958262c3b3b |
| institution | Directory Open Access Journal |
| issn | 2311-6706 2150-5551 |
| language | English |
| last_indexed | 2024-12-10T21:51:37Z |
| publishDate | 2022-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nano-Micro Letters |
| spelling | doaj.art-3e8c6e0daf524eafb7c7a958262c3b3b2022-12-22T01:32:11ZengSpringerOpenNano-Micro Letters2311-67062150-55512022-08-0114111210.1007/s40820-022-00888-4CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta VariantChenshuo Wu0Zhi Chen1Chaozhou Li2Yabin Hao3Yuxuan Tang4Yuxuan Yuan5Luxiao Chai6Taojian Fan7Jiangtian Yu8Xiaopeng Ma9Omar A. Al-Hartomy10S. Wageh11Abdullah G. Al-Sehemi12Zhiguang Luo13Yaqing He14Jingfeng Li15Zhongjian Xie16Han Zhang17International Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen UniversityInternational Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen UniversityInternational Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen UniversityInternational Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen UniversityInternational Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen UniversityInternational Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen UniversityInternational Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen UniversityInternational Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen UniversityShenzhen International Institute for Biomedical ResearchDepartment of Respiratory, Shenzhen Children’s HospitalDepartment of Physics, Faculty of Science, King Abdulaziz UniversityDepartment of Physics, Faculty of Science, King Abdulaziz UniversityResearch Center for Advanced Materials Science (RCAMS), King Khalid UniversityZhongmin (Shenzhen) Intelligent Ecology Co., LtdShenzhen Center for Disease Control and PreventionInternational Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen UniversityShenzhen International Institute for Biomedical ResearchInternational Collaborative Laboratory of 2D, Materials for Optoelectronics Science and Technology of Ministry of Education, Institute of Microscale Optoelectronics, College of Physics and Optoelectronic Engineering, Shenzhen UniversityAbstract Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now, which is time-consuming and requires expensive instrumentation, and the confirmation of variants relies on further sequencing techniques. Herein, we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid, highly sensitivity and specificity for the detection of SARS-CoV-2 variant. To enhance the sensing capability, gold electrodes are uniformly decorated with electro-deposited gold nanoparticles. Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model, our biosensor exhibits excellent analytical detection limit (50 fM) and high linearity (R 2 = 0.987) over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays. The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system. Furthermore, based on the wireless micro-electrochemical platform, the proposed biosensor reveals promising application ability in point-of-care testing.https://doi.org/10.1007/s40820-022-00888-4SARS-COV-2 variantMethodology of electrochemical CRISPR sensing (MOECS)Gold nanoparticles (AuNPs)Point-of-care testing (POCT) |
| spellingShingle | Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao Yuxuan Tang Yuxuan Yuan Luxiao Chai Taojian Fan Jiangtian Yu Xiaopeng Ma Omar A. Al-Hartomy S. Wageh Abdullah G. Al-Sehemi Zhiguang Luo Yaqing He Jingfeng Li Zhongjian Xie Han Zhang CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant Nano-Micro Letters SARS-COV-2 variant Methodology of electrochemical CRISPR sensing (MOECS) Gold nanoparticles (AuNPs) Point-of-care testing (POCT) |
| title | CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant |
| title_full | CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant |
| title_fullStr | CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant |
| title_full_unstemmed | CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant |
| title_short | CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant |
| title_sort | crispr cas12a empowered electrochemical biosensor for rapid and ultrasensitive detection of sars cov 2 delta variant |
| topic | SARS-COV-2 variant Methodology of electrochemical CRISPR sensing (MOECS) Gold nanoparticles (AuNPs) Point-of-care testing (POCT) |
| url | https://doi.org/10.1007/s40820-022-00888-4 |
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