Formulation and In Vivo Evaluation of Biofilm Loaded with Silver Sulfadiazine for Burn Healing
Infected burned skin is a life-threatening condition, which may lead to sepsis. The aims of this work are to formulate a biofilm composed of silver sulfadiazine (SSD), chitosan (CS), and sodium alginate (SA), and to evaluate its wound-healing effectiveness. A full factorial design was used to formul...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/9/11/855 |
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| author | Doaa Alshora Lubna Ashri Rihaf Alfaraj Ahlam Alhusaini Raeesa Mohammad Nawal Alanaze Mohamed Ibrahim Mohamed M. Badran Mounir Bekhit Shaikha Alsaif Modhi Alagili Rehab A. Ali Adel Jreebi |
| author_facet | Doaa Alshora Lubna Ashri Rihaf Alfaraj Ahlam Alhusaini Raeesa Mohammad Nawal Alanaze Mohamed Ibrahim Mohamed M. Badran Mounir Bekhit Shaikha Alsaif Modhi Alagili Rehab A. Ali Adel Jreebi |
| author_sort | Doaa Alshora |
| collection | DOAJ |
| description | Infected burned skin is a life-threatening condition, which may lead to sepsis. The aims of this work are to formulate a biofilm composed of silver sulfadiazine (SSD), chitosan (CS), and sodium alginate (SA), and to evaluate its wound-healing effectiveness. A full factorial design was used to formulate different matrix formulations. The prepared biofilm was tested for physicochemical, and in vitro release. The optimized formulation is composed of 0.833% of CS and 0.75% of SA. The release of SSD almost reached 100% after 6 h. The mechanical properties of the optimized formula were reasonable. The antibacterial activity for the optimized biofilm was significantly higher than that of blank biofilm, which is composed of CS and SA, <i>p</i> = 1.53922 × 10<sup>−12</sup>. Moreover, the in vivo study showed a 75% reduction in wound width when using the formulated SSD biofilm compared to standard marketed cream (57%) and the untreated group (0%). |
| first_indexed | 2024-03-09T16:48:41Z |
| format | Article |
| id | doaj.art-3e8cec49e7d249b9b166ef71e1dad4b8 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T16:48:41Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-3e8cec49e7d249b9b166ef71e1dad4b82023-11-24T14:43:17ZengMDPI AGGels2310-28612023-10-0191185510.3390/gels9110855Formulation and In Vivo Evaluation of Biofilm Loaded with Silver Sulfadiazine for Burn HealingDoaa Alshora0Lubna Ashri1Rihaf Alfaraj2Ahlam Alhusaini3Raeesa Mohammad4Nawal Alanaze5Mohamed Ibrahim6Mohamed M. Badran7Mounir Bekhit8Shaikha Alsaif9Modhi Alagili10Rehab A. Ali11Adel Jreebi12Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Histology, College of Medicine, King Saud University, P.O. Box 2925, Riyadh 11461, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11459, Saudi ArabiaInfected burned skin is a life-threatening condition, which may lead to sepsis. The aims of this work are to formulate a biofilm composed of silver sulfadiazine (SSD), chitosan (CS), and sodium alginate (SA), and to evaluate its wound-healing effectiveness. A full factorial design was used to formulate different matrix formulations. The prepared biofilm was tested for physicochemical, and in vitro release. The optimized formulation is composed of 0.833% of CS and 0.75% of SA. The release of SSD almost reached 100% after 6 h. The mechanical properties of the optimized formula were reasonable. The antibacterial activity for the optimized biofilm was significantly higher than that of blank biofilm, which is composed of CS and SA, <i>p</i> = 1.53922 × 10<sup>−12</sup>. Moreover, the in vivo study showed a 75% reduction in wound width when using the formulated SSD biofilm compared to standard marketed cream (57%) and the untreated group (0%).https://www.mdpi.com/2310-2861/9/11/855silver sulfadiazinebiofilmrelease studyantibacterial activityburn wound model |
| spellingShingle | Doaa Alshora Lubna Ashri Rihaf Alfaraj Ahlam Alhusaini Raeesa Mohammad Nawal Alanaze Mohamed Ibrahim Mohamed M. Badran Mounir Bekhit Shaikha Alsaif Modhi Alagili Rehab A. Ali Adel Jreebi Formulation and In Vivo Evaluation of Biofilm Loaded with Silver Sulfadiazine for Burn Healing Gels silver sulfadiazine biofilm release study antibacterial activity burn wound model |
| title | Formulation and In Vivo Evaluation of Biofilm Loaded with Silver Sulfadiazine for Burn Healing |
| title_full | Formulation and In Vivo Evaluation of Biofilm Loaded with Silver Sulfadiazine for Burn Healing |
| title_fullStr | Formulation and In Vivo Evaluation of Biofilm Loaded with Silver Sulfadiazine for Burn Healing |
| title_full_unstemmed | Formulation and In Vivo Evaluation of Biofilm Loaded with Silver Sulfadiazine for Burn Healing |
| title_short | Formulation and In Vivo Evaluation of Biofilm Loaded with Silver Sulfadiazine for Burn Healing |
| title_sort | formulation and in vivo evaluation of biofilm loaded with silver sulfadiazine for burn healing |
| topic | silver sulfadiazine biofilm release study antibacterial activity burn wound model |
| url | https://www.mdpi.com/2310-2861/9/11/855 |
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