<i>Ex Vivo</i> Raman Spectrochemical Analysis Using a Handheld Probe Demonstrates High Predictive Capability of Brain Tumour Status
With brain tumour incidence increasing, there is an urgent need for better diagnostic tools. Intraoperatively, brain tumours are diagnosed using a smear preparation reported by a neuropathologist. These have many limitations, including the time taken for the specimen to reach the pathology departmen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/9/2/49 |
| _version_ | 1811199974192447488 |
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| author | Danielle Bury Camilo L. M. Morais Katherine M. Ashton Timothy P. Dawson Francis L. Martin |
| author_facet | Danielle Bury Camilo L. M. Morais Katherine M. Ashton Timothy P. Dawson Francis L. Martin |
| author_sort | Danielle Bury |
| collection | DOAJ |
| description | With brain tumour incidence increasing, there is an urgent need for better diagnostic tools. Intraoperatively, brain tumours are diagnosed using a smear preparation reported by a neuropathologist. These have many limitations, including the time taken for the specimen to reach the pathology department and for results to be communicated to the surgeon. There is also a need to assist with resection rates and identifying infiltrative tumour edges intraoperatively to improve clearance. We present a novel study using a handheld Raman probe in conjunction with gold nanoparticles, to detect primary and metastatic brain tumours from fresh brain tissue sent for intraoperative smear diagnosis. Fresh brain tissue samples sent for intraoperative smear diagnosis were tested using the handheld Raman probe after application of gold nanoparticles. Derived Raman spectra were inputted into forward feature extraction algorithms to build a predictive model for sensitivity and specificity of outcome. These results demonstrate an ability to detect primary from metastatic tumours (especially for normal and low grade lesions), in which accuracy, sensitivity and specificity were respectively equal to 98.6%, 94.4% and 99.5% for normal brain tissue; 96.1%, 92.2% and 97.0% for low grade glial tumours; 90.3%, 89.7% and 90.6% for high grade glial tumours; 94.8%, 63.9% and 97.1% for meningiomas; 95.4%, 79.2% and 98.8% for metastases; and 99.6%, 88.9% and 100% for lymphoma, based on smear samples (κ = 0.87). Similar results were observed when compared to the final formalin-fixed paraffin embedded tissue diagnosis (κ = 0.85). Overall, our results have demonstrated the ability of Raman spectroscopy to match results provided by intraoperative smear diagnosis and raise the possibility of use intraoperatively to aid surgeons by providing faster diagnosis. Moving this technology into theatre will allow it to develop further and thus reach its potential in the clinical arena. |
| first_indexed | 2024-04-12T01:56:51Z |
| format | Article |
| id | doaj.art-3e925793c4a8460ca9f9e72031df930c |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-04-12T01:56:51Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-3e925793c4a8460ca9f9e72031df930c2022-12-22T03:52:47ZengMDPI AGBiosensors2079-63742019-03-01924910.3390/bios9020049bios9020049<i>Ex Vivo</i> Raman Spectrochemical Analysis Using a Handheld Probe Demonstrates High Predictive Capability of Brain Tumour StatusDanielle Bury0Camilo L. M. Morais1Katherine M. Ashton2Timothy P. Dawson3Francis L. Martin4School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, UKSchool of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, UKNeuropathology, Royal Preston Hospital, Lancashire Teaching Hospitals NHS Trust, Sharoe Green Lane, Preston PR2 9HT, UKNeuropathology, Royal Preston Hospital, Lancashire Teaching Hospitals NHS Trust, Sharoe Green Lane, Preston PR2 9HT, UKSchool of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, UKWith brain tumour incidence increasing, there is an urgent need for better diagnostic tools. Intraoperatively, brain tumours are diagnosed using a smear preparation reported by a neuropathologist. These have many limitations, including the time taken for the specimen to reach the pathology department and for results to be communicated to the surgeon. There is also a need to assist with resection rates and identifying infiltrative tumour edges intraoperatively to improve clearance. We present a novel study using a handheld Raman probe in conjunction with gold nanoparticles, to detect primary and metastatic brain tumours from fresh brain tissue sent for intraoperative smear diagnosis. Fresh brain tissue samples sent for intraoperative smear diagnosis were tested using the handheld Raman probe after application of gold nanoparticles. Derived Raman spectra were inputted into forward feature extraction algorithms to build a predictive model for sensitivity and specificity of outcome. These results demonstrate an ability to detect primary from metastatic tumours (especially for normal and low grade lesions), in which accuracy, sensitivity and specificity were respectively equal to 98.6%, 94.4% and 99.5% for normal brain tissue; 96.1%, 92.2% and 97.0% for low grade glial tumours; 90.3%, 89.7% and 90.6% for high grade glial tumours; 94.8%, 63.9% and 97.1% for meningiomas; 95.4%, 79.2% and 98.8% for metastases; and 99.6%, 88.9% and 100% for lymphoma, based on smear samples (κ = 0.87). Similar results were observed when compared to the final formalin-fixed paraffin embedded tissue diagnosis (κ = 0.85). Overall, our results have demonstrated the ability of Raman spectroscopy to match results provided by intraoperative smear diagnosis and raise the possibility of use intraoperatively to aid surgeons by providing faster diagnosis. Moving this technology into theatre will allow it to develop further and thus reach its potential in the clinical arena.https://www.mdpi.com/2079-6374/9/2/49brain tumour diagnosisclassificationforward feature extraction algorithmintraoperative useRaman spectroscopyRaman probe |
| spellingShingle | Danielle Bury Camilo L. M. Morais Katherine M. Ashton Timothy P. Dawson Francis L. Martin <i>Ex Vivo</i> Raman Spectrochemical Analysis Using a Handheld Probe Demonstrates High Predictive Capability of Brain Tumour Status Biosensors brain tumour diagnosis classification forward feature extraction algorithm intraoperative use Raman spectroscopy Raman probe |
| title | <i>Ex Vivo</i> Raman Spectrochemical Analysis Using a Handheld Probe Demonstrates High Predictive Capability of Brain Tumour Status |
| title_full | <i>Ex Vivo</i> Raman Spectrochemical Analysis Using a Handheld Probe Demonstrates High Predictive Capability of Brain Tumour Status |
| title_fullStr | <i>Ex Vivo</i> Raman Spectrochemical Analysis Using a Handheld Probe Demonstrates High Predictive Capability of Brain Tumour Status |
| title_full_unstemmed | <i>Ex Vivo</i> Raman Spectrochemical Analysis Using a Handheld Probe Demonstrates High Predictive Capability of Brain Tumour Status |
| title_short | <i>Ex Vivo</i> Raman Spectrochemical Analysis Using a Handheld Probe Demonstrates High Predictive Capability of Brain Tumour Status |
| title_sort | i ex vivo i raman spectrochemical analysis using a handheld probe demonstrates high predictive capability of brain tumour status |
| topic | brain tumour diagnosis classification forward feature extraction algorithm intraoperative use Raman spectroscopy Raman probe |
| url | https://www.mdpi.com/2079-6374/9/2/49 |
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