Global gene expression in two potato cultivars in response to ‘Candidatus Liberibacter solanacearum’ infection
Abstract Background Transcriptomic analyses were performed to compare the molecular responses of two potato varieties previously shown to differ in the severity of disease symptoms due to infection by “Candidatus Liberibacter solanacearum” (Lso), the causative agent of Zebra Chip in potato. A factor...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-12-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-017-4313-2 |
| _version_ | 1828235775923191808 |
|---|---|
| author | Julien G. Levy Azucena Mendoza J. Creighton Miller Cecilia Tamborindeguy Elizabeth A. Pierson |
| author_facet | Julien G. Levy Azucena Mendoza J. Creighton Miller Cecilia Tamborindeguy Elizabeth A. Pierson |
| author_sort | Julien G. Levy |
| collection | DOAJ |
| description | Abstract Background Transcriptomic analyses were performed to compare the molecular responses of two potato varieties previously shown to differ in the severity of disease symptoms due to infection by “Candidatus Liberibacter solanacearum” (Lso), the causative agent of Zebra Chip in potato. A factorial design utilizing the two varieties and psyllids either harboring Lso or without bacteria was used to discriminate varietal responses to pathogen infection versus psyllid feeding. Plant response was determined from leaf samples 3 weeks after infection. Results In response to Lso infection, 397 genes were differentially expressed in the variety Atlantic (most susceptible) as compared to 1027 genes in Waneta. Over 80% of the transcriptionally-changed genes were down-regulated in both varieties, including genes involved in photosynthesis or primary and secondary metabolism. Many of the Lso-responsive genes involved in stress responses or hormonal pathways were regulated differently in the two potato varieties. Conclusions This study focused on the time point just prior to the onset of symptom development and provides valuable insight into the mechanisms of Liberibacter pathogenicity, especially the widespread suppression of plant gene expression, including genes involved in plant defenses. |
| first_indexed | 2024-04-12T20:20:29Z |
| format | Article |
| id | doaj.art-3e946e1e425e4f108ca14480a46ab31e |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T20:20:29Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-3e946e1e425e4f108ca14480a46ab31e2022-12-22T03:18:00ZengBMCBMC Genomics1471-21642017-12-0118111610.1186/s12864-017-4313-2Global gene expression in two potato cultivars in response to ‘Candidatus Liberibacter solanacearum’ infectionJulien G. Levy0Azucena Mendoza1J. Creighton Miller2Cecilia Tamborindeguy3Elizabeth A. Pierson4Department of Horticultural Sciences, Texas A&M UniversityDepartment of Entomology, Texas A&M UniversityDepartment of Horticultural Sciences, Texas A&M UniversityDepartment of Entomology, Texas A&M UniversityDepartment of Horticultural Sciences, Texas A&M UniversityAbstract Background Transcriptomic analyses were performed to compare the molecular responses of two potato varieties previously shown to differ in the severity of disease symptoms due to infection by “Candidatus Liberibacter solanacearum” (Lso), the causative agent of Zebra Chip in potato. A factorial design utilizing the two varieties and psyllids either harboring Lso or without bacteria was used to discriminate varietal responses to pathogen infection versus psyllid feeding. Plant response was determined from leaf samples 3 weeks after infection. Results In response to Lso infection, 397 genes were differentially expressed in the variety Atlantic (most susceptible) as compared to 1027 genes in Waneta. Over 80% of the transcriptionally-changed genes were down-regulated in both varieties, including genes involved in photosynthesis or primary and secondary metabolism. Many of the Lso-responsive genes involved in stress responses or hormonal pathways were regulated differently in the two potato varieties. Conclusions This study focused on the time point just prior to the onset of symptom development and provides valuable insight into the mechanisms of Liberibacter pathogenicity, especially the widespread suppression of plant gene expression, including genes involved in plant defenses.http://link.springer.com/article/10.1186/s12864-017-4313-2TranscriptomePotatoZebra ChipCandidatus Liberibacter solanacearumPsyllids |
| spellingShingle | Julien G. Levy Azucena Mendoza J. Creighton Miller Cecilia Tamborindeguy Elizabeth A. Pierson Global gene expression in two potato cultivars in response to ‘Candidatus Liberibacter solanacearum’ infection BMC Genomics Transcriptome Potato Zebra Chip Candidatus Liberibacter solanacearum Psyllids |
| title | Global gene expression in two potato cultivars in response to ‘Candidatus Liberibacter solanacearum’ infection |
| title_full | Global gene expression in two potato cultivars in response to ‘Candidatus Liberibacter solanacearum’ infection |
| title_fullStr | Global gene expression in two potato cultivars in response to ‘Candidatus Liberibacter solanacearum’ infection |
| title_full_unstemmed | Global gene expression in two potato cultivars in response to ‘Candidatus Liberibacter solanacearum’ infection |
| title_short | Global gene expression in two potato cultivars in response to ‘Candidatus Liberibacter solanacearum’ infection |
| title_sort | global gene expression in two potato cultivars in response to candidatus liberibacter solanacearum infection |
| topic | Transcriptome Potato Zebra Chip Candidatus Liberibacter solanacearum Psyllids |
| url | http://link.springer.com/article/10.1186/s12864-017-4313-2 |
| work_keys_str_mv | AT julienglevy globalgeneexpressionintwopotatocultivarsinresponsetocandidatusliberibactersolanacearuminfection AT azucenamendoza globalgeneexpressionintwopotatocultivarsinresponsetocandidatusliberibactersolanacearuminfection AT jcreightonmiller globalgeneexpressionintwopotatocultivarsinresponsetocandidatusliberibactersolanacearuminfection AT ceciliatamborindeguy globalgeneexpressionintwopotatocultivarsinresponsetocandidatusliberibactersolanacearuminfection AT elizabethapierson globalgeneexpressionintwopotatocultivarsinresponsetocandidatusliberibactersolanacearuminfection |