Molecular characterization of biosynthesis of polyunsaturated fatty acids during different developmental stages in the copepod Apocyclops royi
Copepod Apocyclops royi can biosynthesize long-chain polyunsaturated fatty acids (LC-PUFAs) when fed low-PUFA precursors. Previously, two elongases and two desaturases in the n-3 PUFA biosynthetic pathway were identified from A. royi. However, the complete PUFA biosynthesis pathway in this copepod s...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422000606 |
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| author | Piti Amparyup Supakarn Sungkaew Walaiporn Charoensapsri Paveena Tapaneeyaworawong Parichat Chumtong Patchari Yocawibun Prarthana Pantong Ratree Wongpanya Chanprapa Imjongjirak Sorawit Powtongsook |
| author_facet | Piti Amparyup Supakarn Sungkaew Walaiporn Charoensapsri Paveena Tapaneeyaworawong Parichat Chumtong Patchari Yocawibun Prarthana Pantong Ratree Wongpanya Chanprapa Imjongjirak Sorawit Powtongsook |
| author_sort | Piti Amparyup |
| collection | DOAJ |
| description | Copepod Apocyclops royi can biosynthesize long-chain polyunsaturated fatty acids (LC-PUFAs) when fed low-PUFA precursors. Previously, two elongases and two desaturases in the n-3 PUFA biosynthetic pathway were identified from A. royi. However, the complete PUFA biosynthesis pathway in this copepod species is poorly understood. Here, we report 13 genes, of which nine are novel genes, encoding PUFA biosynthesis-related enzymes belonging to the fatty acid desaturases (ArD6D, ArD5D, ArD4D, ArO3D-1, and ArO3D-2) and elongases (Elovl1, 2, 3, 4, 5, 6, 7, and 8) families identified from a Thai culture of A. royi (A. royi-TH). Identification of the fatty acid contents using gas chromatography/mass spectroscopy analysis indicated that the copepodid and adult stages were high in PUFAs, with omega-3 fatty acids, while the nauplius stage had the lowest level of PUFAs. Moreover, all copepod stages of A. royi-TH fed Tetraselmis suecica contained higher levels of LC-PUFAs, including docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), than the microalgae fatty acid content, which was deficient in omega-3 DHA. Changes in transcript expression levels were determined in three developmental stages of A. royi-TH. Interestingly, the increased gene expression of the fatty acid desaturases (ArD6D, ArD5D, ArD4D, ArO3D-1, and ArO3D-2) and elongases (ArElovl3, 4, 5, 6, and 7) in the adult stages was reflected in the increased fatty acid concentration of DHA and EPA in the adult stages compared with the other developmental stages, suggesting the possible function of these genes for LC-PUFA synthesis in the different copepod developmental stages. These results indicate that the nauplius, copepodid, and adult stages are capable of synthesizing DHA from low PUFA through a LC-PUFA biosynthesis pathway. |
| first_indexed | 2024-12-18T11:07:00Z |
| format | Article |
| id | doaj.art-3e952df87a4547feb2c34329e4ea9c91 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-18T11:07:00Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-3e952df87a4547feb2c34329e4ea9c912022-12-21T21:10:04ZengElsevierAquaculture Reports2352-51342022-04-0123101064Molecular characterization of biosynthesis of polyunsaturated fatty acids during different developmental stages in the copepod Apocyclops royiPiti Amparyup0Supakarn Sungkaew1Walaiporn Charoensapsri2Paveena Tapaneeyaworawong3Parichat Chumtong4Patchari Yocawibun5Prarthana Pantong6Ratree Wongpanya7Chanprapa Imjongjirak8Sorawit Powtongsook9Marine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand; Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Corresponding author at: Marine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.Department of Food Technology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, ThailandMarine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand; Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandMarine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand; Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandMarine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand; Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandMarine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand; Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandMarine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand; Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Biochemistry, Faculty of Science, Kasetsart University, 50 Ngamwongwan Road, Bangkok 10900, ThailandDepartment of Food Technology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand; Corresponding author.Marine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand; Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandCopepod Apocyclops royi can biosynthesize long-chain polyunsaturated fatty acids (LC-PUFAs) when fed low-PUFA precursors. Previously, two elongases and two desaturases in the n-3 PUFA biosynthetic pathway were identified from A. royi. However, the complete PUFA biosynthesis pathway in this copepod species is poorly understood. Here, we report 13 genes, of which nine are novel genes, encoding PUFA biosynthesis-related enzymes belonging to the fatty acid desaturases (ArD6D, ArD5D, ArD4D, ArO3D-1, and ArO3D-2) and elongases (Elovl1, 2, 3, 4, 5, 6, 7, and 8) families identified from a Thai culture of A. royi (A. royi-TH). Identification of the fatty acid contents using gas chromatography/mass spectroscopy analysis indicated that the copepodid and adult stages were high in PUFAs, with omega-3 fatty acids, while the nauplius stage had the lowest level of PUFAs. Moreover, all copepod stages of A. royi-TH fed Tetraselmis suecica contained higher levels of LC-PUFAs, including docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), than the microalgae fatty acid content, which was deficient in omega-3 DHA. Changes in transcript expression levels were determined in three developmental stages of A. royi-TH. Interestingly, the increased gene expression of the fatty acid desaturases (ArD6D, ArD5D, ArD4D, ArO3D-1, and ArO3D-2) and elongases (ArElovl3, 4, 5, 6, and 7) in the adult stages was reflected in the increased fatty acid concentration of DHA and EPA in the adult stages compared with the other developmental stages, suggesting the possible function of these genes for LC-PUFA synthesis in the different copepod developmental stages. These results indicate that the nauplius, copepodid, and adult stages are capable of synthesizing DHA from low PUFA through a LC-PUFA biosynthesis pathway.http://www.sciencedirect.com/science/article/pii/S2352513422000606CopepodApocyclops royiPUFAElongaseDesaturase |
| spellingShingle | Piti Amparyup Supakarn Sungkaew Walaiporn Charoensapsri Paveena Tapaneeyaworawong Parichat Chumtong Patchari Yocawibun Prarthana Pantong Ratree Wongpanya Chanprapa Imjongjirak Sorawit Powtongsook Molecular characterization of biosynthesis of polyunsaturated fatty acids during different developmental stages in the copepod Apocyclops royi Aquaculture Reports Copepod Apocyclops royi PUFA Elongase Desaturase |
| title | Molecular characterization of biosynthesis of polyunsaturated fatty acids during different developmental stages in the copepod Apocyclops royi |
| title_full | Molecular characterization of biosynthesis of polyunsaturated fatty acids during different developmental stages in the copepod Apocyclops royi |
| title_fullStr | Molecular characterization of biosynthesis of polyunsaturated fatty acids during different developmental stages in the copepod Apocyclops royi |
| title_full_unstemmed | Molecular characterization of biosynthesis of polyunsaturated fatty acids during different developmental stages in the copepod Apocyclops royi |
| title_short | Molecular characterization of biosynthesis of polyunsaturated fatty acids during different developmental stages in the copepod Apocyclops royi |
| title_sort | molecular characterization of biosynthesis of polyunsaturated fatty acids during different developmental stages in the copepod apocyclops royi |
| topic | Copepod Apocyclops royi PUFA Elongase Desaturase |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422000606 |
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