RP1S7 – A Possible Marker for Sturgeon Hybrids Identification
The Acipenseridae family includes sturgeon species that have the ability to easily generate interspecies hybrids in both natural and aquaculture conditions. For the differentiation between hybrids and pure species, mitochondrial markers have been used, but they have not proven effective. Nuclear mar...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1019 |
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| author | Gina – Oana Pistol Maria Samu Ramona Nechifor Andreea Dudu Sergiu Emil Georgescu |
| author_facet | Gina – Oana Pistol Maria Samu Ramona Nechifor Andreea Dudu Sergiu Emil Georgescu |
| author_sort | Gina – Oana Pistol |
| collection | DOAJ |
| description | The Acipenseridae family includes sturgeon species that have the ability to easily generate interspecies hybrids in both natural and aquaculture conditions. For the differentiation between hybrids and pure species, mitochondrial markers have been used, but they have not proven effective. Nuclear markers, such as the RP1S7 marker, encoding the S7 ribosomal protein, show to be more effective in achieving this differentiation. The purpose of this study was to evaluate the capacity of this nuclear marker in discriminating between pure sturgeon species and their interspecies hybrids. DNA samples were isolated from fin fragments. The RP1S7 marker was amplified by PCR and the obtained amplicons were analysed by agarose gel electrophoresis and sequencing. In order to determine the accuracy of the primers and to validate this marker, the sequences were compared with similar ones from GenBank. The resulting electrophoretic profiles showed differences that can be used to distinguish between pure sturgeon species and their interspecies hybrids. Therefore, the RP1S7 marker can be included in a panel of DNA markers for molecular identification of sturgeon species and their hybrids. |
| first_indexed | 2024-04-24T11:29:42Z |
| format | Article |
| id | doaj.art-3e953aa4809143bfb11540a5a22b070a |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:29:42Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-3e953aa4809143bfb11540a5a22b070a2024-04-10T10:54:04ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-015111021021019RP1S7 – A Possible Marker for Sturgeon Hybrids IdentificationGina – Oana Pistol0Maria Samu1Ramona Nechifor2Andreea Dudu3Sergiu Emil Georgescu4University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, 91-95 Splaiul Independenței, 050095, Bucharest, RomaniaThe Acipenseridae family includes sturgeon species that have the ability to easily generate interspecies hybrids in both natural and aquaculture conditions. For the differentiation between hybrids and pure species, mitochondrial markers have been used, but they have not proven effective. Nuclear markers, such as the RP1S7 marker, encoding the S7 ribosomal protein, show to be more effective in achieving this differentiation. The purpose of this study was to evaluate the capacity of this nuclear marker in discriminating between pure sturgeon species and their interspecies hybrids. DNA samples were isolated from fin fragments. The RP1S7 marker was amplified by PCR and the obtained amplicons were analysed by agarose gel electrophoresis and sequencing. In order to determine the accuracy of the primers and to validate this marker, the sequences were compared with similar ones from GenBank. The resulting electrophoretic profiles showed differences that can be used to distinguish between pure sturgeon species and their interspecies hybrids. Therefore, the RP1S7 marker can be included in a panel of DNA markers for molecular identification of sturgeon species and their hybrids.https://spasb.ro/index.php/public_html/article/view/1019sturgeonshybridizationnuclear markersrp1s7 |
| spellingShingle | Gina – Oana Pistol Maria Samu Ramona Nechifor Andreea Dudu Sergiu Emil Georgescu RP1S7 – A Possible Marker for Sturgeon Hybrids Identification Scientific Papers Animal Science and Biotechnologies sturgeons hybridization nuclear markers rp1s7 |
| title | RP1S7 – A Possible Marker for Sturgeon Hybrids Identification |
| title_full | RP1S7 – A Possible Marker for Sturgeon Hybrids Identification |
| title_fullStr | RP1S7 – A Possible Marker for Sturgeon Hybrids Identification |
| title_full_unstemmed | RP1S7 – A Possible Marker for Sturgeon Hybrids Identification |
| title_short | RP1S7 – A Possible Marker for Sturgeon Hybrids Identification |
| title_sort | rp1s7 a possible marker for sturgeon hybrids identification |
| topic | sturgeons hybridization nuclear markers rp1s7 |
| url | https://spasb.ro/index.php/public_html/article/view/1019 |
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