Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens.
Infection with reticuloendotheliosis virus (REV), a gammaretrovirus in the Retroviridae family, can result in immunosuppression and subsequent increased susceptibility to secondary infections. The effects of REV infection on expression of mRNA for cytokine genes in chickens have not been completely...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3865284?pdf=render |
| _version_ | 1818061415872724992 |
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| author | Mei Xue Xingming Shi Yan Zhao Hongyu Cui Shunlei Hu Xianlan Cui Yunfeng Wang |
| author_facet | Mei Xue Xingming Shi Yan Zhao Hongyu Cui Shunlei Hu Xianlan Cui Yunfeng Wang |
| author_sort | Mei Xue |
| collection | DOAJ |
| description | Infection with reticuloendotheliosis virus (REV), a gammaretrovirus in the Retroviridae family, can result in immunosuppression and subsequent increased susceptibility to secondary infections. The effects of REV infection on expression of mRNA for cytokine genes in chickens have not been completely elucidated. In this study, using multiplex branched DNA (bDNA) technology, we identified molecular mediators that participated in the regulation of the immune response during REV infection in chickens. Cytokine and chemokine mRNA expression levels were evaluated in the peripheral blood mononuclear cells (PBMCs). Expression levels of interleukin (IL)-4, IL-10, IL-13 and tumor necrosis factor (TNF)-α were significantly up-regulated while interferon (IFN)-α, IFN-β, IFN-γ, IL-1β, IL-2, IL-3, IL-15, IL-17F, IL-18 and colony-stimulating factor (CSF)-1 were markedly decreased in PBMCs at all stages of infection. Compared with controls, REV infected chickens showed greater expression levels of IL-8 in PBMCs 21 and 28 days post infection. In addition, REV regulates host immunity as a suppressor of T cell proliferative responses. The results in this study will help us to understand the host immune response to virus pathogens. |
| first_indexed | 2024-12-10T13:47:57Z |
| format | Article |
| id | doaj.art-3e9d2afe483b435baf03bcfa999ec0a2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T13:47:57Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3e9d2afe483b435baf03bcfa999ec0a22022-12-22T01:46:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8391810.1371/journal.pone.0083918Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens.Mei XueXingming ShiYan ZhaoHongyu CuiShunlei HuXianlan CuiYunfeng WangInfection with reticuloendotheliosis virus (REV), a gammaretrovirus in the Retroviridae family, can result in immunosuppression and subsequent increased susceptibility to secondary infections. The effects of REV infection on expression of mRNA for cytokine genes in chickens have not been completely elucidated. In this study, using multiplex branched DNA (bDNA) technology, we identified molecular mediators that participated in the regulation of the immune response during REV infection in chickens. Cytokine and chemokine mRNA expression levels were evaluated in the peripheral blood mononuclear cells (PBMCs). Expression levels of interleukin (IL)-4, IL-10, IL-13 and tumor necrosis factor (TNF)-α were significantly up-regulated while interferon (IFN)-α, IFN-β, IFN-γ, IL-1β, IL-2, IL-3, IL-15, IL-17F, IL-18 and colony-stimulating factor (CSF)-1 were markedly decreased in PBMCs at all stages of infection. Compared with controls, REV infected chickens showed greater expression levels of IL-8 in PBMCs 21 and 28 days post infection. In addition, REV regulates host immunity as a suppressor of T cell proliferative responses. The results in this study will help us to understand the host immune response to virus pathogens.http://europepmc.org/articles/PMC3865284?pdf=render |
| spellingShingle | Mei Xue Xingming Shi Yan Zhao Hongyu Cui Shunlei Hu Xianlan Cui Yunfeng Wang Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. PLoS ONE |
| title | Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. |
| title_full | Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. |
| title_fullStr | Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. |
| title_full_unstemmed | Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. |
| title_short | Effects of reticuloendotheliosis virus infection on cytokine production in SPF chickens. |
| title_sort | effects of reticuloendotheliosis virus infection on cytokine production in spf chickens |
| url | http://europepmc.org/articles/PMC3865284?pdf=render |
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