Oxidative Stress and DNA Damage Effect of <i>Dioscorea hispida</i> Dennst. on Placental Tissues of Rats
<i>Dioscorea hispida</i> Dennst. locally known as “ubi gadung” has been used as a traditional remedy and source of carbohydrate among Malaysians. To assess the effect of <i>Dioscorea hispida</i> aqueous extract (DHAE) on the production of reactive oxygen species (ROS) and the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/7/2190 |
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| author | Hussin Muhammad Tengku Aideed Syah Tg Abu Bakar Muhamad Faizul Adhzim Yusery Norizah Awang Wan Mazlina Md. Saad Elda Nurafnie Ibnu Rasid Mohamad Fawzi Mahomoodally Maizatul Hasyima Omar |
| author_facet | Hussin Muhammad Tengku Aideed Syah Tg Abu Bakar Muhamad Faizul Adhzim Yusery Norizah Awang Wan Mazlina Md. Saad Elda Nurafnie Ibnu Rasid Mohamad Fawzi Mahomoodally Maizatul Hasyima Omar |
| author_sort | Hussin Muhammad |
| collection | DOAJ |
| description | <i>Dioscorea hispida</i> Dennst. locally known as “ubi gadung” has been used as a traditional remedy and source of carbohydrate among Malaysians. To assess the effect of <i>Dioscorea hispida</i> aqueous extract (DHAE) on the production of reactive oxygen species (ROS) and their effects on DNA damage in Sprague Dawley rat’s placental tissues, pregnant rats were randomly divided into four groups. The animals were orally treated with distilled water (negative control) and three different concentrations of DHAE (250, 500 and 1000 mg/kg body weight (BW)) from gestation day 6 until 20. The oxidative stress in placental tissues was evaluated at day 21 by measuring the level of ROS, superoxide dismutase (SOD) and lipid peroxidation biomarker, malondialdehyde (MDA) while comet assay was used for DNA damage. There was no significant production of ROS and SOD activities in all groups. Significant changes were observed in the MDA level at 1000 mg/kg BW DHAE. Comet assay revealed a significant increase (<i>p</i> < 0.05) of DNA damage on animals treated with 250 and 500 mg/kg BW DHAE but not at the highest concentration. It was postulated that the placental cells could have undergone necrosis which destroys all components including DNA. This occurrence simultaneously reduces the levels of DNA damage which can be represented by lower level of tail moments. This finding correlates with our histopathological examination where necrotic cells of spongiotrophoblast were observed in the basal zone of placental tissue. The high amount of hydrogen cyanide and other compounds in 1000 mg/kg BW DHAE could elevate the lipid peroxidation and directly induce cell necrosis which requires further investigation. |
| first_indexed | 2024-03-09T11:36:50Z |
| format | Article |
| id | doaj.art-3e9d8dd91e0d417a8cf309611be030ac |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:36:50Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3e9d8dd91e0d417a8cf309611be030ac2023-11-30T23:40:49ZengMDPI AGMolecules1420-30492022-03-01277219010.3390/molecules27072190Oxidative Stress and DNA Damage Effect of <i>Dioscorea hispida</i> Dennst. on Placental Tissues of RatsHussin Muhammad0Tengku Aideed Syah Tg Abu Bakar1Muhamad Faizul Adhzim Yusery2Norizah Awang3Wan Mazlina Md. Saad4Elda Nurafnie Ibnu Rasid5Mohamad Fawzi Mahomoodally6Maizatul Hasyima Omar7Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, Shah Alam 40170, Selangor, MalaysiaDepartment of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA UiTM Puncak Alam, Bandar Puncak Alam 42300, Selangor, MalaysiaDepartment of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA UiTM Puncak Alam, Bandar Puncak Alam 42300, Selangor, MalaysiaHerbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, Shah Alam 40170, Selangor, MalaysiaDepartment of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA UiTM Puncak Alam, Bandar Puncak Alam 42300, Selangor, MalaysiaHerbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, Shah Alam 40170, Selangor, MalaysiaDepartment of Health Sciences, Faculty of Medicine and Health Science, University of Mauritius, Réduit 80837, MauritiusHerbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, Shah Alam 40170, Selangor, Malaysia<i>Dioscorea hispida</i> Dennst. locally known as “ubi gadung” has been used as a traditional remedy and source of carbohydrate among Malaysians. To assess the effect of <i>Dioscorea hispida</i> aqueous extract (DHAE) on the production of reactive oxygen species (ROS) and their effects on DNA damage in Sprague Dawley rat’s placental tissues, pregnant rats were randomly divided into four groups. The animals were orally treated with distilled water (negative control) and three different concentrations of DHAE (250, 500 and 1000 mg/kg body weight (BW)) from gestation day 6 until 20. The oxidative stress in placental tissues was evaluated at day 21 by measuring the level of ROS, superoxide dismutase (SOD) and lipid peroxidation biomarker, malondialdehyde (MDA) while comet assay was used for DNA damage. There was no significant production of ROS and SOD activities in all groups. Significant changes were observed in the MDA level at 1000 mg/kg BW DHAE. Comet assay revealed a significant increase (<i>p</i> < 0.05) of DNA damage on animals treated with 250 and 500 mg/kg BW DHAE but not at the highest concentration. It was postulated that the placental cells could have undergone necrosis which destroys all components including DNA. This occurrence simultaneously reduces the levels of DNA damage which can be represented by lower level of tail moments. This finding correlates with our histopathological examination where necrotic cells of spongiotrophoblast were observed in the basal zone of placental tissue. The high amount of hydrogen cyanide and other compounds in 1000 mg/kg BW DHAE could elevate the lipid peroxidation and directly induce cell necrosis which requires further investigation.https://www.mdpi.com/1420-3049/27/7/2190<i>Dioscorea hispida</i>comet assayoxidative stressreactive oxygen speciesplacental tissue |
| spellingShingle | Hussin Muhammad Tengku Aideed Syah Tg Abu Bakar Muhamad Faizul Adhzim Yusery Norizah Awang Wan Mazlina Md. Saad Elda Nurafnie Ibnu Rasid Mohamad Fawzi Mahomoodally Maizatul Hasyima Omar Oxidative Stress and DNA Damage Effect of <i>Dioscorea hispida</i> Dennst. on Placental Tissues of Rats Molecules <i>Dioscorea hispida</i> comet assay oxidative stress reactive oxygen species placental tissue |
| title | Oxidative Stress and DNA Damage Effect of <i>Dioscorea hispida</i> Dennst. on Placental Tissues of Rats |
| title_full | Oxidative Stress and DNA Damage Effect of <i>Dioscorea hispida</i> Dennst. on Placental Tissues of Rats |
| title_fullStr | Oxidative Stress and DNA Damage Effect of <i>Dioscorea hispida</i> Dennst. on Placental Tissues of Rats |
| title_full_unstemmed | Oxidative Stress and DNA Damage Effect of <i>Dioscorea hispida</i> Dennst. on Placental Tissues of Rats |
| title_short | Oxidative Stress and DNA Damage Effect of <i>Dioscorea hispida</i> Dennst. on Placental Tissues of Rats |
| title_sort | oxidative stress and dna damage effect of i dioscorea hispida i dennst on placental tissues of rats |
| topic | <i>Dioscorea hispida</i> comet assay oxidative stress reactive oxygen species placental tissue |
| url | https://www.mdpi.com/1420-3049/27/7/2190 |
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